AB188320

Anti-EMAP II/AIMP1 antibody [EPR14168(B)]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal EMAP II/AIMP1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

View Alternative Names

EMAP2, SCYE1, AIMP1, Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, Multisynthase complex auxiliary component p43

3 Images

Supplier Data

Western blot - Anti-EMAP II/AIMP1 antibody [EPR14168(B)] (AB188320)

All lanes:

Western blot - Anti-EMAP II/AIMP1 antibody [EPR14168(B)] (ab188320) at 1/5000 dilution

Lane 1:

HT-1080 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Supplier Data

Western blot - Anti-EMAP II/AIMP1 antibody [EPR14168(B)] (AB188320)

All lanes:

Western blot - Anti-EMAP II/AIMP1 antibody [EPR14168(B)] (ab188320) at 1/1000 dilution

All lanes:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Supplier Data

Western blot - Anti-EMAP II/AIMP1 antibody [EPR14168(B)] (AB188320)

All lanes:

Western blot - Anti-EMAP II/AIMP1 antibody [EPR14168(B)] (ab188320) at 1/1000 dilution

Lane 1:

Mouse kidney tissue lysate at 10 µg

Lane 2:

Mouse spleen tissue lysate at 10 µg

Lane 3:

C6 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Carrier free

Anti-EMAP II/AIMP1 antibody [EPR14168(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14168(B)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/60", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Perform heat mediated antigen retrieval using EDTA Buffer, pH9 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/60", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Perform heat mediated antigen retrieval using EDTA Buffer, pH9 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/60", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Perform heat mediated antigen retrieval using EDTA Buffer, pH9 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EMAP II also known as AIMP1 (Aminoacyl tRNA Synthetase Complex-Interacting Multifunctional Protein 1) is a protein with a mass of approximately 34 kDa. Mechanically it modulates various cellular processes including apoptosis and inflammation. This protein is expressed in several tissues with higher levels seen in the immune cells the lung and the heart. AIMP1 is part of the wider group of proteins involved in the multi-synthetase complex which ensures efficient protein synthesis.

Biological function summary

AIMP1 functions beyond its role in the multi-synthetase complex to affect cellular proliferation and differentiation. It acts as a cytokine that can influence immune responses. When secreted AIMP1 shows pro-inflammatory activity targeting endothelial cells macrophages and fibroblasts. It is important for regulating the immune system's response to stress and external stimuli thereby bridging cellular activities with immune signaling networks.

Pathways

AIMP1 integrates into both the inflammatory response pathway and apoptosis signaling. This protein interacts with TNF-alpha and IL-6 which are important players in inflammation. AIMP1's activity influences ERK and JNK pathways contributing to the regulation of apoptosis and survival of immune cells. Its relation with these pathways highlights its role as a mediator that can shift cellular balance towards survival or programmed cell death based on external cues.

AIMP1 has connections to cancer and autoimmune diseases. Studies show increased levels of AIMP1 in tumors linking it to cancer progression especially in breast cancer where it interacts with proteins like p53. Furthermore AIMP1's involvement in autoimmune disorders such as rheumatoid arthritis connects it with TNF-alpha an important inflammatory mediator. This connection suggests AIMP1's function as a potential biomarker and target for therapeutic interventions in such conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase (PubMed : 10358004). Binds tRNA. Possesses inflammatory cytokine activity (PubMed : 11306575). Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation (By similarity). Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels (By similarity). Promotes dermal fibroblast proliferation and wound repair (PubMed : 16472771). Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum (By similarity). Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelial cell apoptosis at high concentrations (PubMed : 12237313). Induces maturation of dendritic cells and monocyte cell adhesion (PubMed : 11818442). Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7 (PubMed : 19362550).

See full target information AIMP1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

BMC geriatrics 25:645 PubMed40836274

2025

AIMP1 exerts hearing protection role in age related hearing loss mice by regulating SIRT1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Haisen Peng,Jiali Liu,Yuehui Liu,Chunhua Li,Zhilin Zhang,Shuihua Hu,Wen Xie

American journal of cancer research 15:2535-2550 PubMed40667556

2025

Exploring the role of YES1 kinase in regulating cisplatin resistance through iTRAQ-based quantitative proteomic analysis in urothelial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Che Wu,Chung-Wen Kuo,Ming-Chun Kuo,Chang-Ting Lin,Ling-Yi Xiao,Li-Chung Chang,Yi-Hua Chen,Shih-Yu Huang,Harvey Yu-Li Su

Viruses 15: PubMed36851687

2023

HIV-1 Gag Binds the Multi-Aminoacyl-tRNA Synthetase Complex via the EPRS Subunit.

Applications

Unspecified application

Species

Unspecified reactive species

Danni Jin,Yiping Zhu,Heidi L Schubert,Stephen P Goff,Karin Musier-Forsyth

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EMAP II/AIMP1 antibody [EPR14168(B)]

Western blot - Anti-EMAP II/AIMP1 antibody [EPR14168(B)] (AB188320)

Western blot - Anti-EMAP II/AIMP1 antibody [EPR14168(B)] (AB188320)

Western blot - Anti-EMAP II/AIMP1 antibody [EPR14168(B)] (AB188320)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise