AB250950

Anti-EMAP II/AIMP1 antibody [EPR14168(B)] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal EMAP II/AIMP1 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

View Alternative Names

EMAP2, SCYE1, AIMP1, Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, Multisynthase complex auxiliary component p43

3 Images

Supplier Data

Western blot - Anti-EMAP II/AIMP1 antibody [EPR14168(B)] - BSA and Azide free (AB250950)

This data was developed using ab188320, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EMAP II/AIMP1 antibody [EPR14168(B)] (<a href='/en-us/products/primary-antibodies/emap-ii-aimp1-antibody-epr14168b-ab188320'>ab188320</a>) at 1/5000 dilution

Lane 1:

HT-1080 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Supplier Data

Western blot - Anti-EMAP II/AIMP1 antibody [EPR14168(B)] - BSA and Azide free (AB250950)

This data was developed using ab188320, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EMAP II/AIMP1 antibody [EPR14168(B)] (<a href='/en-us/products/primary-antibodies/emap-ii-aimp1-antibody-epr14168b-ab188320'>ab188320</a>) at 1/1000 dilution

All lanes:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Supplier Data

Western blot - Anti-EMAP II/AIMP1 antibody [EPR14168(B)] - BSA and Azide free (AB250950)

This data was developed using ab188320, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EMAP II/AIMP1 antibody [EPR14168(B)] (<a href='/en-us/products/primary-antibodies/emap-ii-aimp1-antibody-epr14168b-ab188320'>ab188320</a>) at 1/1000 dilution

Lane 1:

Mouse kidney tissue lysate at 10 µg

Lane 2:

Mouse spleen tissue lysate at 10 µg

Lane 3:

C6 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Unconjugated

Anti-EMAP II/AIMP1 antibody [EPR14168(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14168(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

ab250950 is the carrier-free version of ab188320.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EMAP II also known as AIMP1 (Aminoacyl tRNA Synthetase Complex-Interacting Multifunctional Protein 1) is a protein with a mass of approximately 34 kDa. Mechanically it modulates various cellular processes including apoptosis and inflammation. This protein is expressed in several tissues with higher levels seen in the immune cells the lung and the heart. AIMP1 is part of the wider group of proteins involved in the multi-synthetase complex which ensures efficient protein synthesis.

Biological function summary

AIMP1 functions beyond its role in the multi-synthetase complex to affect cellular proliferation and differentiation. It acts as a cytokine that can influence immune responses. When secreted AIMP1 shows pro-inflammatory activity targeting endothelial cells macrophages and fibroblasts. It is important for regulating the immune system's response to stress and external stimuli thereby bridging cellular activities with immune signaling networks.

Pathways

AIMP1 integrates into both the inflammatory response pathway and apoptosis signaling. This protein interacts with TNF-alpha and IL-6 which are important players in inflammation. AIMP1's activity influences ERK and JNK pathways contributing to the regulation of apoptosis and survival of immune cells. Its relation with these pathways highlights its role as a mediator that can shift cellular balance towards survival or programmed cell death based on external cues.

AIMP1 has connections to cancer and autoimmune diseases. Studies show increased levels of AIMP1 in tumors linking it to cancer progression especially in breast cancer where it interacts with proteins like p53. Furthermore AIMP1's involvement in autoimmune disorders such as rheumatoid arthritis connects it with TNF-alpha an important inflammatory mediator. This connection suggests AIMP1's function as a potential biomarker and target for therapeutic interventions in such conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase (PubMed : 10358004). Binds tRNA. Possesses inflammatory cytokine activity (PubMed : 11306575). Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation (By similarity). Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels (By similarity). Promotes dermal fibroblast proliferation and wound repair (PubMed : 16472771). Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum (By similarity). Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelial cell apoptosis at high concentrations (PubMed : 12237313). Induces maturation of dendritic cells and monocyte cell adhesion (PubMed : 11818442). Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7 (PubMed : 19362550).

See full target information AIMP1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Endocrine-related cancer 25:35-50 PubMed29042395

2017

Nuclear receptor profiling in prostatospheroids and castration-resistant prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhu Wang,Dinglan Wu,Chi-Fai Ng,Jeremy Yuen-Chun Teoh,Shan Yu,Yuliang Wang,Franky L Chan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EMAP II/AIMP1 antibody [EPR14168(B)] - BSA and Azide free

Western blot - Anti-EMAP II/AIMP1 antibody [EPR14168(B)] - BSA and Azide free (AB250950)

Western blot - Anti-EMAP II/AIMP1 antibody [EPR14168(B)] - BSA and Azide free (AB250950)

Western blot - Anti-EMAP II/AIMP1 antibody [EPR14168(B)] - BSA and Azide free (AB250950)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise