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AB250110

Anti-Embigin homolog antibody [EPR11417] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Embigin homolog antibody. Carrier free. Suitable for IP, Flow Cyt, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Embigin, EMB

3 Images
Flow Cytometry - Anti-Embigin homolog antibody [EPR11417] - BSA and Azide free (AB250110)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Embigin homolog antibody [EPR11417] - BSA and Azide free (AB250110)

This data was developed using ab179801, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 293T cells labeling Embigin homolog with ab179801 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Immunoprecipitation - Anti-Embigin homolog antibody [EPR11417] - BSA and Azide free (AB250110)
  • IP

Supplier Data

Immunoprecipitation - Anti-Embigin homolog antibody [EPR11417] - BSA and Azide free (AB250110)

This data was developed using ab179801, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate using ab179801.

All lanes:

Immunoprecipitation - Anti-Embigin homolog antibody [EPR11417] (<a href='/en-us/products/primary-antibodies/embigin-homolog-antibody-epr11417-ab179801'>ab179801</a>)

Predicted band size: 37 kDa

false

Western blot - Anti-Embigin homolog antibody [EPR11417] - BSA and Azide free (AB250110)
  • WB

Supplier Data

Western blot - Anti-Embigin homolog antibody [EPR11417] - BSA and Azide free (AB250110)

This data was developed using ab179801, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Embigin homolog antibody [EPR11417] (<a href='/en-us/products/primary-antibodies/embigin-homolog-antibody-epr11417-ab179801'>ab179801</a>) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Lane 4:

LNCaP cell lysate at 10 µg

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11417

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab250110 is the carrier-free version of ab179801.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Embigin homolog protein often referred to simply as Embigin plays an important mechanical role within the cell. It is a transmembrane protein with a molecular mass of approximately 41 kDa. Researchers have observed Embigin expressed mostly in the tissues of the developing central nervous system and muscle tissues. It is also detected in adult tissues but in distinct expression patterns including the heart and the testis.
Biological function summary

Embigin is involved in the regulation of cell adhesion and proliferation. It is not generally part of a larger protein complex but acts as a facilitator of cellular interactions particularly during tissue morphogenesis and development. Embigin's role in promoting cell aggregation involves interaction with other cell surface proteins influencing cellular dynamics during development.

Pathways

Embigin plays a role in the integrin signaling pathway. It interacts with integrins which are transmembrane receptors involved in cell adhesion sending signals that regulate the cytoskeleton and the cell cycle. Another pathway related to Embigin's function is the PI3K/Akt signaling pathway. Through this pathway Embigin indirectly influences cellular survival and growth processes closely interacting with signaling proteins like PI3K.

Researchers have linked Embigin to cancer and neurodegenerative conditions. Changes in Embigin expression have been connected to various cancers as the protein influences cell adhesion and migration which are critical in tumor progression. In neurodegenerative diseases altered expression of Embigin affects cellular interactions in neural tissues relating to proteins like the neural cell adhesion molecule which further impacts neural health and connectivity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the outgrowth of motoneurons and in the formation of neuromuscular junctions. Following muscle denervation, promotes nerve terminal sprouting and the formation of additional acetylcholine receptor clusters at synaptic sites without affecting terminal Schwann cell number or morphology. Delays the retraction of terminal sprouts following re-innervation of denervated endplates. May play a role in targeting the monocarboxylate transporters SLC16A1, SLC16A6 and SLC16A7 to the cell membrane (By similarity).
See full target information EMB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2207435 PubMed38286681

2024

Defective Homologous Recombination Repair By Up-Regulating Lnc-HZ10/Ahr Loop in Human Trophoblast Cells Induced Miscarriage.

Applications

Unspecified application

Species

Unspecified reactive species

Weina Chen,Chenyang Mi,Ying Zhang,Yang Yang,Wenxin Huang,Zhongyan Xu,Jingsong Zhao,Rong Wang,Manli Wang,Shukun Wan,Xiaoqing Wang,Huidong Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com