AB184544

Anti-EMC4 antibody [EPR15080]

RabMAb
Recombinant
What is this?

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(7 Publications)

Rabbit Recombinant Monoclonal EMC4 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 7 publications.

View Alternative Names

TMEM85, HSPC184, PIG17, EMC4, ER membrane protein complex subunit 4, Cell proliferation-inducing gene 17 protein, Transmembrane protein 85

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (AB184544)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (AB184544)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue labeling EMC4 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (AB184544)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling EMC4 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-EMC4 antibody [EPR15080] (AB184544)

Western blot analysis of EMC4 in HepG2 cell lysate immunoprecipitated using ab184544 at 1/50 dilution (Lane 1). Lane 2 : PBS.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-EMC4 antibody [EPR15080] (ab184544)

Predicted band size: 20 kDa

Observed band size: 17 kDa,20 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (AB184544)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling EMC4 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-EMC4 antibody [EPR15080] (AB184544)

All lanes:

Western blot - Anti-EMC4 antibody [EPR15080] (ab184544) at 1/10000 dilution

Lane 1:

293 cell lysate at 20 µg

Lane 2:

SH-SY5Y cell lysate at 20 µg

Lane 3:

U87-MG cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 17 kDa,20 kDa

false

Carrier free

Anti-EMC4 antibody [EPR15080] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15080

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Based on the sequence analysis, this antibody recognizes three isoforms with the predicted MWs of 20KDa, 17KDa and 15KDa, respectively.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50 - 1/70", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EMC4 also known as Endoplasmic Reticulum Membrane Protein Complex Subunit 4 is a protein involved in the insertion of membrane proteins into the endoplasmic reticulum (ER). It has a molecular mass of approximately 29 kDa and is expressed widespread in various tissues. EMC4 is part of the EMC complex a multi-subunit complex in the ER contributing to the processes of protein folding and assembly in the cell's secretory pathway.

Biological function summary

The EMC4 protein functions as a chaperone assisting in the proper folding and integration of polytopic proteins. It is a part of the EMC complex that works as a stabilizing agent for nascent polypeptides preventing misfolding and facilitating protein maturation. This complex plays a role in maintaining cellular homeostasis and challenges the ER stress response ensuring that proteins reach their functional conformation.

Pathways

EMC4 has a critical role in the protein secretory pathway and ER-associated degradation (ERAD) pathway. The EMC complex interacts with signal recognition particles and the translocon complex supporting the translocation and integration of proteins into the ER membrane. This involvement is key to processing newly synthesized proteins correctly collaborating with proteins like Sec61 which form part of the translocon complex.

Anomalies in EMC4 expression or function have connections to neurodegenerative diseases and congenital disorders of glycosylation. Altered EMC4 function can disrupt ER homeostasis contributing to neurodegenerative conditions like Alzheimer's disease where it relates to proteins such as amyloid precursor protein (APP). Furthermore EMC4 mutations may lead to glycosylation defects impacting the proper functioning of proteins involved in cellular communication and structure.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed : 29242231, PubMed : 29809151, PubMed : 30415835, PubMed : 32439656, PubMed : 32459176). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed : 29242231, PubMed : 29809151, PubMed : 30415835). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed : 29809151, PubMed : 30415835). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed : 29242231, PubMed : 29809151). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed : 30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable).

See full target information EMC4

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7097 PubMed40753078

2025

The EMC acts as a chaperone for membrane proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Carolin J Klose,Kevin M Meighen-Berger,Martin Kulke,Marina Parr,Barbara Steigenberger,Martin Zacharias,Dmitrij Frishman,Matthias J Feige

iScience 26:105667 PubMed36624844

2023

EMC3 regulates mesenchymal cell survival via control of the mitotic spindle assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofang Tang,Wei Wei,John M Snowball,Ernesto S Nakayasu,Sheila M Bell,Charles Ansong,Xinhua Lin,Jeffrey A Whitsett

Cell death and differentiation 30:500-514 PubMed36481789

2022

Deficiency of cancer/testis antigen gene CT55 causes male infertility in humans and mice.

Applications

Unspecified application

Species

Unspecified reactive species

Guohui Zhang,Chuan Jiang,Yushang Yang,Yan Wang,Haimeng Zhou,Siyu Dai,Mohan Liu,Yanting Yang,Li Yang,Qiongyan Shen,Tao Zhang,Xiaodong Zhang,Yihong Yang,Ying Shen

eLife 9: PubMed33236988

2020

Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients.

Applications

Unspecified application

Species

Unspecified reactive species

Lakshmi E Miller-Vedam,Bastian Bräuning,Katerina D Popova,Nicole T Schirle Oakdale,Jessica L Bonnar,Jesuraj R Prabu,Elizabeth A Boydston,Natalia Sevillano,Matthew J Shurtleff,Robert M Stroud,Charles S Craik,Brenda A Schulman,Adam Frost,Jonathan S Weissman

Nucleic acids research 48:9872-9885 PubMed32890404

2020

Ribosomal stalk proteins RPLP1 and RPLP2 promote biogenesis of flaviviral and cellular multi-pass transmembrane proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael K Campos,H R Sagara Wijeratne,Premal Shah,Mariano A Garcia-Blanco,Shelton S Bradrick

Cell reports 28:2517-2526.e5 PubMed31484065

2019

Proteomic Analysis Identifies Membrane Proteins Dependent on the ER Membrane Protein Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Songhai Tian,Quan Wu,Bo Zhou,Mei Yuk Choi,Bo Ding,Wei Yang,Min Dong

Nature communications 10:672 PubMed30737405

2019

A network of chaperones prevents and detects failures in membrane protein lipid bilayer integration.

Applications

Unspecified application

Species

Unspecified reactive species

João P L Coelho,Matthias Stahl,Nicolas Bloemeke,Kevin Meighen-Berger,Carlos Piedrafita Alvira,Zai-Rong Zhang,Stephan A Sieber,Matthias J Feige

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EMC4 antibody [EPR15080]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (AB184544)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (AB184544)

Immunoprecipitation - Anti-EMC4 antibody [EPR15080] (AB184544)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (AB184544)

Western blot - Anti-EMC4 antibody [EPR15080] (AB184544)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise