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AB88878

Anti-EME1 antibody

1

(2 Reviews)

|

(3 Publications)

Mouse Polyclonal EME1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EME1.

View Alternative Names

MMS4, EME1, Crossover junction endonuclease EME1, MMS4 homolog, hMMS4

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-EME1 antibody (AB88878)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EME1 antibody (AB88878)

ab88878 at 10 μg/ml staining EME1 in HeLa cells by immunofluorescence.

Western blot - Anti-EME1 antibody (AB88878)
  • WB

Unknown

Western blot - Anti-EME1 antibody (AB88878)

All lanes:

Western blot - Anti-EME1 antibody (ab88878) at 1 µg/mL

All lanes:

HeLa cell lysate at 50 µg

Predicted band size: 63 kDa

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Western blot - Anti-EME1 antibody (AB88878)
  • WB

Unknown

Western blot - Anti-EME1 antibody (AB88878)

All lanes:

Western blot - Anti-EME1 antibody (ab88878) at 1 µg/mL

All lanes:

Jurkat cell lysate at 50 µg

Predicted band size: 63 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human EME1.

Q96AY2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The EME1 protein also known as Essential Meiotic Endonuclease 1 plays a mechanical role in the DNA repair process. It has a molecular mass of approximately 58 kDa. EME1 is ubiquitously expressed in various tissues which suggests its broad importance in cellular functions. Mechanically it forms part of the XPF-EME1 endonuclease complex where it partners with the XPF protein to facilitate repair of DNA interstrand crosslinks and the resolution of DNA joint molecules during homologous recombination.
Biological function summary

EME1 participates in maintaining genomic stability. As a component of the XPF-EME1 complex it executes incision of branched DNA structures important for DNA repair processes such as nucleotide excision repair and interstrand crosslink repair. Additionally it contributes to safeguarding the integrity of replicating DNA. Through these biological actions EME1 ensures the prevention of genome instability which could otherwise lead to cellular dysfunction.

Pathways

EME1 operates within the DNA repair pathways notably the nucleotide excision repair (NER) and the homologous recombination repair (HRR) pathways. In NER EME1 partners with the XPF protein enabling precise corrections of damaged DNA. In the HRR pathway EME1 interacts with other repair proteins to facilitate appropriate chromosomal segregation and DNA replication. Consequently its action underpins these pathways' essential roles in fixing DNA damages and maintaining genetic accuracy.

Mutations or dysfunctions in EME1 have links to increased susceptibility to cancers and genomic instability disorders like Fanconi anemia. The protein's integral role in DNA repair connects it to several other proteins such as BRCA2 which collaborates with EME1 in homologous recombination. When EME1 function is impaired these collaborations break down contributing to the pathogenesis of these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
See full target information EME1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Psychopharmacology 240:2617-2629 PubMed37707611

2023

Group I and group II metabotropic glutamate receptors are upregulated in the synapses of infant rats prenatally exposed to valproic acid.

Applications

Unspecified application

Species

Unspecified reactive species

Simona D'Antoni,Sara Schiavi,Valeria Buzzelli,Samuele Giuffrida,Alessandro Feo,Fabrizio Ascone,Carla Letizia Busceti,Ferdinando Nicoletti,Viviana Trezza,Maria Vincenza Catania

Nature communications 13:4240 PubMed35869071

2022

TDP1-independent pathways in the process and repair of TOP1-induced DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Zhang,Yun Xiong,Dan Su,Chao Wang,Mrinal Srivastava,Mengfan Tang,Xu Feng,Min Huang,Zhen Chen,Junjie Chen

Bioengineered 12:9869-9884 PubMed34719326

2021

Essential meiotic structure-specific endonuclease1 () promotes malignant features in gastric cancer cells via the Akt/GSK3B/CCND1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiguo Guo,Erbo Liang,Wei Li,Leilei Jiang,Fachao Zhi
View all publications
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Product promise

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