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AB153718

Anti-Emerin antibody

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(2 Publications)

Rabbit Polyclonal Emerin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EMD aa 1 to C-terminus.

View Alternative Names

EDMD, STA, EMD, Emerin

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emerin antibody (AB153718)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emerin antibody (AB153718)

Immunohistochemical analysis of paraffin-embedded Human HeLa xenograft tissue, labeling Emerin with ab153718 at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Emerin antibody (AB153718)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Emerin antibody (AB153718)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells, labeling Emerin with ab153718 at 1/500 dilution.

Western blot - Anti-Emerin antibody (AB153718)
  • WB

Unknown

Western blot - Anti-Emerin antibody (AB153718)

12% SDS PAGE

All lanes:

Western blot - Anti-Emerin antibody (ab153718) at 1/5000 dilution

All lanes:

HeLa nuclear lysate at 30 µg

Predicted band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EMD aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P50402

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Alternative Products

Primary Antibodies

AB156871

Anti-Emerin antibody [EPR11071]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emerin antibody [EPR11071] (AB156871)

  • 0
  • 0
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Anti-Emerin antibody [8F5A8]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emerin antibody [EPR11071] - BSA and Azide free (AB240138)

  • 0
  • 0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Emerin also referred to as the emerin protein is a nuclear envelope protein with a mass of approximately 34 kDa. It belongs to a family of LEM domain proteins that localize at the inner nuclear membrane. Emerin predominantly expresses in cardiac and skeletal muscles but also appears in other tissues. Its primary mechanical role involves binding to the nuclear lamina structures and interacting with various nuclear proteins to maintain nuclear integrity and architecture.
Biological function summary

Emerin serves as an important component in mechanical signal transduction pathways. It interacts with barrier-to-autointegration factor (BAF) forming an important part of the nuclear envelope complex. This protein complex helps in chromatin organization and regulation of gene expression. Emerin influences nuclear assembly and shape and modulates chromatin attachment to the nuclear envelope playing an important role in nuclear processes.

Pathways

Emerin participates actively in the Emery-Dreifuss muscular dystrophy pathway and affects the interplay of other nuclear lamina components like lamin A/C. It stabilizes chromatin structure and cooperates with proteins such as nesprins and SUN proteins to regulate nuclear-cytoskeletal interactions. Emerin's involvement in these pathways highlights its role in maintaining mechanical resilience and functionality of the nuclear envelope impacting gene expression dynamics.

Emerin mutations relate to Emery-Dreifuss muscular dystrophy (EDMD) and other musculoskeletal disorders. Alteration in emerin disrupts its interaction with lamin A/C and BAF leading to nuclear envelope defects. This dysfunction manifests in muscle weakness and cardiac anomalies typical of EDMD. Understanding emerin's role offers insights into pathogenesis and potential therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. Required for proper localization of non-farnesylated prelamin-A/C. Together with NEMP1, contributes to nuclear envelope stiffness in germ cells (PubMed : 32923640). EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.
See full target information EMD
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 10: PubMed34944023

2021

The Methyltransferase Smyd1 Mediates LPS-Triggered Up-Regulation of IL-6 in Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Shamloul,Gustav Steinemann,Kerrin Roos,Celine Huajia Liem,Jonathan Bernd,Thorsten Braun,Andreas Zakrzewicz,Janine Berkholz

PLoS genetics 14:e1007845 PubMed30543681

2018

Biallelic mutations in nucleoporin NUP88 cause lethal fetal akinesia deformation sequence.

Applications

Unspecified application

Species

Unspecified reactive species

Edith Bonnin,Pauline Cabochette,Alessandro Filosa,Ramona Jühlen,Shoko Komatsuzaki,Mohammed Hezwani,Achim Dickmanns,Valérie Martinelli,Marjorie Vermeersch,Lynn Supply,Nuno Martins,Laurence Pirenne,Gianina Ravenscroft,Marcus Lombard,Sarah Port,Christiane Spillner,Sandra Janssens,Ellen Roets,Jo Van Dorpe,Martin Lammens,Ralph H Kehlenbach,Ralf Ficner,Nigel G Laing,Katrin Hoffmann,Benoit Vanhollebeke,Birthe Fahrenkrog
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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