Anti-Emerin antibody [EPR11071] - BSA and Azide free

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Emerin antibody [EPR11071] - BSA and Azide free (AB240138)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Emerin with Purified ab156871 at 1/30 dilution (10μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156871).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emerin antibody [EPR11071] - BSA and Azide free (AB240138)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling Emerin with unpurified ab156871 at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156871).

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emerin antibody [EPR11071] - BSA and Azide free (AB240138)

Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling Emerin with unpurified ab156871 at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156871).

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Emerin antibody [EPR11071] - BSA and Azide free (AB240138)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Emerin with Purified ab156871 at 1 : 50 dilution (6.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156871).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emerin antibody [EPR11071] - BSA and Azide free (AB240138)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue sections labeling Emerin with purified ab156871 at 1/500 dilution (0.652 μg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156871).

• WB

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Western blot - Anti-Emerin antibody [EPR11071] - BSA and Azide free (AB240138)

Blocking/Diluting buffer : 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156871).

All lanes:

Western blot - Anti-Emerin antibody [EPR11071] (<a href='/en-us/products/primary-antibodies/emerin-antibody-epr11071-ab156871'>ab156871</a>) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 35 kDa

false

• WB

Lab

Western blot - Anti-Emerin antibody [EPR11071] - BSA and Azide free (AB240138)

This data was developed using the same antibody clone in a different buffer formulation (ab156871).

Lanes 1-2 : Merged signal (red and green). Green - ab156871 observed at 35 kDa. Red - loading control ab8245 observed at 37 kDa.

ab156871 Anti-Emerin antibody [EPR11071] was shown to specifically react with Emerin in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266336 (knockout cell lysate ab257423) was used. Wild-type and Emerin knockout samples were subjected to SDS-PAGE. ab156871 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Emerin antibody [EPR11071] (<a href='/en-us/products/primary-antibodies/emerin-antibody-epr11071-ab156871'>ab156871</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

EMD knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human EMD (Emerin) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-emd-emerin-knockout-hek-293t-cell-line-ab266336'>ab266336</a>)

Predicted band size: 29 kDa,51 kDa,72 kDa

Observed band size: 35 kDa,51 kDa

false