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AB215765

Anti-Emi1 antibody [EMI1/1176]

1

(1 Review)

|

(2 Publications)

Mouse Monoclonal Emi1 antibody. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FBXO5 aa 1-250.

View Alternative Names

EMI1, FBX5, FBXO5, F-box only protein 5, Early mitotic inhibitor 1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EMI1/1176] (AB215765)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EMI1/1176] (AB215765)

Immunohistochemistry analysis of paraffin embedded, formalin fixed Human ovarian carcinoma labeling Emi1 using ab215765 at 1 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EMI1/1176] (AB215765)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EMI1/1176] (AB215765)

Immunohistochemistry analysis of paraffin embedded, formalin fixed Human ovarian carcinoma labeling Emi1 using ab215765 at 1 μg/ml.

Western blot - Anti-Emi1 antibody [EMI1/1176] (AB215765)
  • WB

Supplier Data

Western blot - Anti-Emi1 antibody [EMI1/1176] (AB215765)

All lanes:

Western blot - Anti-Emi1 antibody [EMI1/1176] (ab215765) at 2 µg/mL

Lane 1:

HeLa cell lysates

Lane 2:

HepG2 cell lysates

Lane 3:

HEK293 cell lysates

Lane 4:

K562 cell lysates

Predicted band size: 50 kDa

false

Protein Array - Anti-Emi1 antibody [EMI1/1176] (AB215765)
  • Protein Array

Unknown

Protein Array - Anti-Emi1 antibody [EMI1/1176] (AB215765)

ab215765 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

EMI1/1176

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, Protein Array

applications

Immunogen

Recombinant Fragment Protein within Human FBXO5 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9UKT4

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Emi1 also known as FBXO5 is a regulatory protein involved in cell cycle control with a molecular mass of approximately 48 kDa. It functions by inhibiting the anaphase-promoting complex/cyclosome (APC/C) an important E3 ubiquitin ligase that tags proteins for degradation during mitosis. Emi1 is expressed in a variety of tissues including those with actively dividing cells like bone marrow and lymph nodes. The regulatory role of Emi1 ensures the proper timing of mitosis preventing premature progression to anaphase.
Biological function summary

Emi1 serves as an essential cell cycle regulator by controlling the activity of the APC/C therefore maintaining genomic stability during cell division. It acts independently not as part of a larger protein complex ensuring that cyclin B and securin levels remain sufficient to hold the cell in metaphase until all chromosomes are correctly attached to the spindle apparatus. This function prevents chromosomal instability and aneuploidy which can lead to malignancies.

Pathways

Emi1 plays a critical role in the cell cycle regulatory network by interacting with the APC/C complex. By regulating the degradation of cell cycle proteins Emi1 helps maintain the balance between cell proliferation and apoptosis. The regulatory influence of Emi1 on the APC/C involves interactions with Cdc20 and Cdh1 two key activators of APC/C which ensures proper cell cycle progression and checkpoint control.

Alterations in Emi1 expression or function can contribute to the development of cancer and other proliferative disorders. Emi1 overexpression is frequently found in many cancers including breast and prostate cancer where it contributes to unchecked cell proliferation. Through its interaction with the APC/C complex aberrant Emi1 activity can disrupt normal cell cycle control mechanisms leading to tumorigenesis. The protein's role in preventing APC/C-mediated degradation also makes it a connecting factor to proteins like cyclin B linking Emi1 to processes involved in oncogenesis.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Regulator of APC activity during mitotic and meiotic cell cycle (PubMed : 16921029, PubMed : 17234884, PubMed : 17485488, PubMed : 17875940, PubMed : 23708001, PubMed : 23708605). During mitotic cell cycle plays a role as both substrate and inhibitor of APC-FZR1 complex (PubMed : 16921029, PubMed : 17234884, PubMed : 17485488, PubMed : 17875940, PubMed : 23708001, PubMed : 23708605, PubMed : 29875408). During G1 phase, plays a role as substrate of APC-FZR1 complex E3 ligase (PubMed : 29875408). Then switches as an inhibitor of APC-FZR1 complex during S and G2 leading to cell-cycle commitment (PubMed : 29875408). As APC inhibitor, prevents the degradation of APC substrates at multiple levels : by interacting with APC and blocking access of APC substrates to the D-box coreceptor, formed by FZR1 and ANAPC10; by suppressing ubiquitin ligation and chain elongation by APC by preventing the UBE2C and UBE2S activities (PubMed : 16921029, PubMed : 23708001, PubMed : 23708605). Plays a role in genome integrity preservation by coordinating DNA replication with mitosis through APC inhibition in interphase to stabilize CCNA2 and GMNN in order to promote mitosis and prevent rereplication and DNA damage-induced cellular senescence (PubMed : 17234884, PubMed : 17485488, PubMed : 17875940). During oocyte maturation, plays a role in meiosis through inactivation of APC-FZR1 complex. Inhibits APC through RPS6KA2 interaction that increases FBXO5 affiniy for CDC20 leading to the metaphase arrest of the second meiotic division before fertilization (By similarity). Controls entry into the first meiotic division through inactivation of APC-FZR1 complex (By similarity). Promotes migration and osteogenic differentiation of mesenchymal stem cells (PubMed : 29850565).
See full target information FBXO5

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature biotechnology 43:52-62 PubMed38418648

2024

Morphological diversification and functional maturation of human astrocytes in glia-enriched cortical organoid transplanted in mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Meiyan Wang,Lei Zhang,Sammy Weiser Novak,Jingting Yu,Iryna S Gallina,Lynne L Xu,Christina K Lim,Sarah Fernandes,Maxim N Shokhirev,April E Williams,Monisha D Saxena,Shashank Coorapati,Sarah L Parylak,Cristian Quintero,Elsa Molina,Leonardo R Andrade,Uri Manor,Fred H Gage

BMC molecular and cell biology 24:34 PubMed38041032

2023

Effect of Emi1 gene silencing on the proliferation and invasion of human breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Kuang,Shengwen Huang,Shifan Tang,Zhaozhen Zhuo,Keyan Linghu
View all publications

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