Anti-Emi1 antibody [EMI1/1176]
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(1 Review)
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(2 Publications)
Mouse Monoclonal Emi1 antibody. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FBXO5 aa 1-250.
View Alternative Names
EMI1, FBX5, FBXO5, F-box only protein 5, Early mitotic inhibitor 1
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EMI1/1176] (AB215765)
Immunohistochemistry analysis of paraffin embedded, formalin fixed Human ovarian carcinoma labeling Emi1 using ab215765 at 1 μg/ml.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EMI1/1176] (AB215765)
Immunohistochemistry analysis of paraffin embedded, formalin fixed Human ovarian carcinoma labeling Emi1 using ab215765 at 1 μg/ml.
- WB
Supplier Data
Western blot - Anti-Emi1 antibody [EMI1/1176] (AB215765)
All lanes:
Western blot - Anti-Emi1 antibody [EMI1/1176] (ab215765) at 2 µg/mL
Lane 1:
HeLa cell lysates
Lane 2:
HepG2 cell lysates
Lane 3:
HEK293 cell lysates
Lane 4:
K562 cell lysates
Predicted band size: 50 kDa
false
- Protein Array
Unknown
Protein Array - Anti-Emi1 antibody [EMI1/1176] (AB215765)
ab215765 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Reactivity data
Properties and storage information
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Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Emi1 serves as an essential cell cycle regulator by controlling the activity of the APC/C therefore maintaining genomic stability during cell division. It acts independently not as part of a larger protein complex ensuring that cyclin B and securin levels remain sufficient to hold the cell in metaphase until all chromosomes are correctly attached to the spindle apparatus. This function prevents chromosomal instability and aneuploidy which can lead to malignancies.
Pathways
Emi1 plays a critical role in the cell cycle regulatory network by interacting with the APC/C complex. By regulating the degradation of cell cycle proteins Emi1 helps maintain the balance between cell proliferation and apoptosis. The regulatory influence of Emi1 on the APC/C involves interactions with Cdc20 and Cdh1 two key activators of APC/C which ensures proper cell cycle progression and checkpoint control.
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Target data
Publications (2)
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Nature biotechnology 43:52-62 PubMed38418648
2024
Applications
Unspecified application
Species
Unspecified reactive species
BMC molecular and cell biology 24:34 PubMed38041032
2023
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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