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AB187144

Anti-Emi1 antibody [EPR15320-103]

3

(2 Reviews)

|

(2 Publications)

Rabbit Recombinant Monoclonal Emi1 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 2 publications.

View Alternative Names

EMI1, FBX5, FBXO5, F-box only protein 5, Early mitotic inhibitor 1

1 Images
Western blot - Anti-Emi1 antibody [EPR15320-103] (AB187144)
  • WB

Supplier Data

Western blot - Anti-Emi1 antibody [EPR15320-103] (AB187144)

All lanes:

Western blot - Anti-Emi1 antibody [EPR15320-103] (ab187144) at 1/10000 dilution

Lane 1:

Hela cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 56 kDa

false

  • Carrier free

    Anti-Emi1 antibody [EPR15320-103] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15320-103

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Emi1 also known as FBXO5 is a regulatory protein involved in cell cycle control with a molecular mass of approximately 48 kDa. It functions by inhibiting the anaphase-promoting complex/cyclosome (APC/C) an important E3 ubiquitin ligase that tags proteins for degradation during mitosis. Emi1 is expressed in a variety of tissues including those with actively dividing cells like bone marrow and lymph nodes. The regulatory role of Emi1 ensures the proper timing of mitosis preventing premature progression to anaphase.
Biological function summary

Emi1 serves as an essential cell cycle regulator by controlling the activity of the APC/C therefore maintaining genomic stability during cell division. It acts independently not as part of a larger protein complex ensuring that cyclin B and securin levels remain sufficient to hold the cell in metaphase until all chromosomes are correctly attached to the spindle apparatus. This function prevents chromosomal instability and aneuploidy which can lead to malignancies.

Pathways

Emi1 plays a critical role in the cell cycle regulatory network by interacting with the APC/C complex. By regulating the degradation of cell cycle proteins Emi1 helps maintain the balance between cell proliferation and apoptosis. The regulatory influence of Emi1 on the APC/C involves interactions with Cdc20 and Cdh1 two key activators of APC/C which ensures proper cell cycle progression and checkpoint control.

Alterations in Emi1 expression or function can contribute to the development of cancer and other proliferative disorders. Emi1 overexpression is frequently found in many cancers including breast and prostate cancer where it contributes to unchecked cell proliferation. Through its interaction with the APC/C complex aberrant Emi1 activity can disrupt normal cell cycle control mechanisms leading to tumorigenesis. The protein's role in preventing APC/C-mediated degradation also makes it a connecting factor to proteins like cyclin B linking Emi1 to processes involved in oncogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulator of APC activity during mitotic and meiotic cell cycle (PubMed : 16921029, PubMed : 17234884, PubMed : 17485488, PubMed : 17875940, PubMed : 23708001, PubMed : 23708605). During mitotic cell cycle plays a role as both substrate and inhibitor of APC-FZR1 complex (PubMed : 16921029, PubMed : 17234884, PubMed : 17485488, PubMed : 17875940, PubMed : 23708001, PubMed : 23708605, PubMed : 29875408). During G1 phase, plays a role as substrate of APC-FZR1 complex E3 ligase (PubMed : 29875408). Then switches as an inhibitor of APC-FZR1 complex during S and G2 leading to cell-cycle commitment (PubMed : 29875408). As APC inhibitor, prevents the degradation of APC substrates at multiple levels : by interacting with APC and blocking access of APC substrates to the D-box coreceptor, formed by FZR1 and ANAPC10; by suppressing ubiquitin ligation and chain elongation by APC by preventing the UBE2C and UBE2S activities (PubMed : 16921029, PubMed : 23708001, PubMed : 23708605). Plays a role in genome integrity preservation by coordinating DNA replication with mitosis through APC inhibition in interphase to stabilize CCNA2 and GMNN in order to promote mitosis and prevent rereplication and DNA damage-induced cellular senescence (PubMed : 17234884, PubMed : 17485488, PubMed : 17875940). During oocyte maturation, plays a role in meiosis through inactivation of APC-FZR1 complex. Inhibits APC through RPS6KA2 interaction that increases FBXO5 affiniy for CDC20 leading to the metaphase arrest of the second meiotic division before fertilization (By similarity). Controls entry into the first meiotic division through inactivation of APC-FZR1 complex (By similarity). Promotes migration and osteogenic differentiation of mesenchymal stem cells (PubMed : 29850565).
See full target information FBXO5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 24:e70105 PubMed40371663

2025

hTERT Increases TRF2 to Induce Telomere Compaction and Extend Cell Replicative Lifespan.

Applications

Unspecified application

Species

Unspecified reactive species

Nancy Adam,Yang Yang,Mahbod Djamshidi,Sara Seifan,Nicholas S Y Ting,Joel Glover,Nicolas Touret,Paul M K Gordon,K V Vineetha Warriyar,Hokan Krowicki,Christine Kim Garcia,Sharon A Savage,Aaron A Goodarzi,Duncan M Baird,Tara L Beattie,Karl Riabowol

BioMed research international 2018:7849294 PubMed29850565

2018

Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Liu,Kun Liu,Yanzhe Yan,Zhuangzhuang Chu,Yi Tang,Chunbo Tang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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