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AB250788

Anti-Emi1 antibody [EPR15320] - BSA and Azide free

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Rabbit Recombinant Monoclonal Emi1 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.

View Alternative Names

EMI1, FBX5, FBXO5, F-box only protein 5, Early mitotic inhibitor 1

4 Images
Flow Cytometry (Intracellular) - Anti-Emi1 antibody [EPR15320] - BSA and Azide free (AB250788)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Emi1 antibody [EPR15320] - BSA and Azide free (AB250788)

This data was developed using ab184950, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling Emi1 with ab184950 at 1/180 dilution (red). Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control (green).

Immunocytochemistry/ Immunofluorescence - Anti-Emi1 antibody [EPR15320] - BSA and Azide free (AB250788)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Emi1 antibody [EPR15320] - BSA and Azide free (AB250788)

This data was developed using ab184950, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling Emi1 with ab184950 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) at 1/200 dilution was used as the secondary antibody. The slide on the right is counter stained with Dapi (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EPR15320] - BSA and Azide free (AB250788)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EPR15320] - BSA and Azide free (AB250788)

This data was developed using ab184950, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling Emi1 with ab184950 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-Emi1 antibody [EPR15320] - BSA and Azide free (AB250788)
  • WB

Supplier Data

Western blot - Anti-Emi1 antibody [EPR15320] - BSA and Azide free (AB250788)

This data was developed using ab184950, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Emi1 antibody [EPR15320] (<a href='/en-us/products/primary-antibodies/emi1-antibody-epr15320-ab184950'>ab184950</a>) at 1/5000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15320

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab250788 is the carrier-free version of ab184950.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Emi1 also known as FBXO5 is a regulatory protein involved in cell cycle control with a molecular mass of approximately 48 kDa. It functions by inhibiting the anaphase-promoting complex/cyclosome (APC/C) an important E3 ubiquitin ligase that tags proteins for degradation during mitosis. Emi1 is expressed in a variety of tissues including those with actively dividing cells like bone marrow and lymph nodes. The regulatory role of Emi1 ensures the proper timing of mitosis preventing premature progression to anaphase.
Biological function summary

Emi1 serves as an essential cell cycle regulator by controlling the activity of the APC/C therefore maintaining genomic stability during cell division. It acts independently not as part of a larger protein complex ensuring that cyclin B and securin levels remain sufficient to hold the cell in metaphase until all chromosomes are correctly attached to the spindle apparatus. This function prevents chromosomal instability and aneuploidy which can lead to malignancies.

Pathways

Emi1 plays a critical role in the cell cycle regulatory network by interacting with the APC/C complex. By regulating the degradation of cell cycle proteins Emi1 helps maintain the balance between cell proliferation and apoptosis. The regulatory influence of Emi1 on the APC/C involves interactions with Cdc20 and Cdh1 two key activators of APC/C which ensures proper cell cycle progression and checkpoint control.

Alterations in Emi1 expression or function can contribute to the development of cancer and other proliferative disorders. Emi1 overexpression is frequently found in many cancers including breast and prostate cancer where it contributes to unchecked cell proliferation. Through its interaction with the APC/C complex aberrant Emi1 activity can disrupt normal cell cycle control mechanisms leading to tumorigenesis. The protein's role in preventing APC/C-mediated degradation also makes it a connecting factor to proteins like cyclin B linking Emi1 to processes involved in oncogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulator of APC activity during mitotic and meiotic cell cycle (PubMed : 16921029, PubMed : 17234884, PubMed : 17485488, PubMed : 17875940, PubMed : 23708001, PubMed : 23708605). During mitotic cell cycle plays a role as both substrate and inhibitor of APC-FZR1 complex (PubMed : 16921029, PubMed : 17234884, PubMed : 17485488, PubMed : 17875940, PubMed : 23708001, PubMed : 23708605, PubMed : 29875408). During G1 phase, plays a role as substrate of APC-FZR1 complex E3 ligase (PubMed : 29875408). Then switches as an inhibitor of APC-FZR1 complex during S and G2 leading to cell-cycle commitment (PubMed : 29875408). As APC inhibitor, prevents the degradation of APC substrates at multiple levels : by interacting with APC and blocking access of APC substrates to the D-box coreceptor, formed by FZR1 and ANAPC10; by suppressing ubiquitin ligation and chain elongation by APC by preventing the UBE2C and UBE2S activities (PubMed : 16921029, PubMed : 23708001, PubMed : 23708605). Plays a role in genome integrity preservation by coordinating DNA replication with mitosis through APC inhibition in interphase to stabilize CCNA2 and GMNN in order to promote mitosis and prevent rereplication and DNA damage-induced cellular senescence (PubMed : 17234884, PubMed : 17485488, PubMed : 17875940). During oocyte maturation, plays a role in meiosis through inactivation of APC-FZR1 complex. Inhibits APC through RPS6KA2 interaction that increases FBXO5 affiniy for CDC20 leading to the metaphase arrest of the second meiotic division before fertilization (By similarity). Controls entry into the first meiotic division through inactivation of APC-FZR1 complex (By similarity). Promotes migration and osteogenic differentiation of mesenchymal stem cells (PubMed : 29850565).
See full target information FBXO5
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com