AB231052

Anti-EMILIN1 antibody

Name: Anti-EMILIN1 antibody (ab231052) | Abcam
Brand: Abcam Limited
SKU: AB231052
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal EMILIN1 antibody. Suitable for WB, IHC-P and reacts with Rat samples. Cited in 2 publications.

View Alternative Names

EMI, EMILIN1, EMILIN-1, Elastin microfibril interface-located protein 1, Elastin microfibril interfacer 1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMILIN1 antibody (AB231052)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMILIN1 antibody (AB231052)

Paraffin-embedded rat spleen tissue stained for EMILIN1 using ab231052 at 20 μg/ml in immunohistochemical analysis, followed by DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMILIN1 antibody (AB231052)

Paraffin-embedded rat spleen tissue stained for EMILIN1 using ab231052 at 20 μg/ml in immunohistochemical analysis, followed by DAB staining.

Supplier Data

Western blot - Anti-EMILIN1 antibody (AB231052)

All lanes:

Western blot - Anti-EMILIN1 antibody (ab231052) at 3 µg/mL

Lane 1:

Rat serum

Lane 2:

Rat intestine lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit antibody at 1/2000 dilution

Predicted band size: 107 kDa

false

Supplier Data

Western blot - Anti-EMILIN1 antibody (AB231052)

All lanes:

Western blot - Anti-EMILIN1 antibody (ab231052) at 3 µg/mL

All lanes:

Recombinant rat EMILIN1 protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit antibody at 1/2000 dilution

Predicted band size: 107 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

IHC-P, WB

applications

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab231052 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EMILIN1 also known as elastin microfibril interface located protein 1 is a glycoprotein that plays a role in the extracellular matrix. The protein has a molecular mass of approximately 120 kDa. It is expressed in several tissues with high levels observed in vascular tissues skin and other connective tissues. EMILIN1 interacts with other matrix proteins which helps maintain the structural integrity of tissues.

Biological function summary

EMILIN1 contributes to maintaining elastogenesis and modulating cell adhesion by interacting with integrins. It forms part of the extracellular matrix where it supports elastin fibers which gives elasticity to tissues. EMILIN1 also influences cell behavior by modulating the signaling pathways through its binding to integrins. The proper function of EMILIN1 is important for tissue elasticity and cell migration.

Pathways

EMILIN1 is integral to the Notch signaling and TGF-β pathways. It interacts with Notch receptors affecting cell differentiation and proliferation. Additionally its interplay with TGF-β signaling regulates extracellular matrix organization and angiogenesis. These connections link EMILIN1 to proteins like TGF-β and Notch receptors highlighting its role in maintaining cellular and tissue homeostasis.

Defects or altered expression of EMILIN1 have been implicated in cardiovascular diseases and skin disorders. It has connections to proteins such as integrins and TGF-β which are also involved in these conditions. In cardiovascular disorders EMILIN1 dysfunction can lead to improper vessel formation and elasticity contributing to hypertension and atherosclerosis. Skin disorders involving EMILIN1 may present as conditions with compromised elastic fiber architecture such as cutis laxa.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Involved in elastic and collagen fibers formation. It is required for EFEMP2 deposition into the extracellular matrix, and collagen network assembly and cross-linking via protein-lysine 6-oxidase/LOX activity (PubMed : 36351433). May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved in the processes that regulate vessel assembly. Has cell adhesive capacity.

See full target information EMILIN1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bioengineering (Basel, Switzerland) 11: PubMed38927779

2024

Generation of Tailored Extracellular Matrix Hydrogels for the Study of In Vitro Folliculogenesis in Response to Matrisome-Dependent Biochemical Cues.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah B McDowell,Kathryn L McElhinney,Elizabeth L Tsui,Monica M Laronda

Frontiers in immunology 13:873382 PubMed35720361

2022

Adiponectin Ameliorates GMH-Induced Brain Injury by Regulating Microglia M1/M2 Polarization AdipoR1/APPL1/AMPK/PPARγ Signaling Pathway in Neonatal Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ningbo Xu,Xifeng Li,Jun Weng,Chunhua Wei,Zhenyan He,Desislava Met Doycheva,Cameron Lenahan,Wenhui Tang,Jian Zhou,Yanchao Liu,Qiang Xu,Yahong Liu,Xuying He,Jiping Tang,John H Zhang,Chuanzhi Duan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EMILIN1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMILIN1 antibody (AB231052)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMILIN1 antibody (AB231052)

Western blot - Anti-EMILIN1 antibody (AB231052)

Western blot - Anti-EMILIN1 antibody (AB231052)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise