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AB250857

Anti-EMILIN1 antibody [EPR14678] - BSA and Azide free

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Rabbit Recombinant Monoclonal EMILIN1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

EMI, EMILIN1, EMILIN-1, Elastin microfibril interface-located protein 1, Elastin microfibril interfacer 1

2 Images
Western blot - Anti-EMILIN1 antibody [EPR14678] - BSA and Azide free (AB250857)
  • WB

Supplier Data

Western blot - Anti-EMILIN1 antibody [EPR14678] - BSA and Azide free (AB250857)

This data was developed using ab185953, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EMILIN1 antibody [EPR14678] - N-terminal (<a href='/en-us/products/primary-antibodies/emilin1-antibody-epr14678-n-terminal-ab185953'>ab185953</a>) at 1/50000 dilution

Lane 1:

Human uterus tissue lysate at 20 µg

Lane 2:

Human fetal kidney tissue lysate at 20 µg

Lane 3:

Human colon cancer tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 107 kDa

false

Immunoprecipitation - Anti-EMILIN1 antibody [EPR14678] - BSA and Azide free (AB250857)
  • IP

Supplier Data

Immunoprecipitation - Anti-EMILIN1 antibody [EPR14678] - BSA and Azide free (AB250857)

This data was developed using ab185953, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from Human fetal kidney tissue lysate labeling EMILIN1 with ab185953 at a 1/50 dilution. Secondary ab : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated, at a 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-EMILIN1 antibody [EPR14678] - N-terminal (<a href='/en-us/products/primary-antibodies/emilin1-antibody-epr14678-n-terminal-ab185953'>ab185953</a>)

Predicted band size: 107 kDa

false

  • Unconjugated

    Anti-EMILIN1 antibody [EPR14678] - N-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14678

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250857 is the carrier-free version of ab185953.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EMILIN1 also known as elastin microfibril interface located protein 1 is a glycoprotein that plays a role in the extracellular matrix. The protein has a molecular mass of approximately 120 kDa. It is expressed in several tissues with high levels observed in vascular tissues skin and other connective tissues. EMILIN1 interacts with other matrix proteins which helps maintain the structural integrity of tissues.
Biological function summary

EMILIN1 contributes to maintaining elastogenesis and modulating cell adhesion by interacting with integrins. It forms part of the extracellular matrix where it supports elastin fibers which gives elasticity to tissues. EMILIN1 also influences cell behavior by modulating the signaling pathways through its binding to integrins. The proper function of EMILIN1 is important for tissue elasticity and cell migration.

Pathways

EMILIN1 is integral to the Notch signaling and TGF-β pathways. It interacts with Notch receptors affecting cell differentiation and proliferation. Additionally its interplay with TGF-β signaling regulates extracellular matrix organization and angiogenesis. These connections link EMILIN1 to proteins like TGF-β and Notch receptors highlighting its role in maintaining cellular and tissue homeostasis.

Defects or altered expression of EMILIN1 have been implicated in cardiovascular diseases and skin disorders. It has connections to proteins such as integrins and TGF-β which are also involved in these conditions. In cardiovascular disorders EMILIN1 dysfunction can lead to improper vessel formation and elasticity contributing to hypertension and atherosclerosis. Skin disorders involving EMILIN1 may present as conditions with compromised elastic fiber architecture such as cutis laxa.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity.
See full target information EMILIN1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com