AB185953

Anti-EMILIN1 antibody [EPR14678] - N-terminal

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal EMILIN1 antibody. N-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

EMI, EMILIN1, EMILIN-1, Elastin microfibril interface-located protein 1, Elastin microfibril interfacer 1

2 Images

Supplier Data

Immunoprecipitation - Anti-EMILIN1 antibody [EPR14678] - N-terminal (AB185953)

Western blot analysis of immunoprecipitation pellet from Human fetal kidney tissue lysate labeling EMILIN1 with ab185953 at a 1/50 dilution. Secondary ab : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated, at a 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-EMILIN1 antibody [EPR14678] - N-terminal (ab185953)

Predicted band size: 107 kDa

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Supplier Data

Western blot - Anti-EMILIN1 antibody [EPR14678] - N-terminal (AB185953)

All lanes:

Western blot - Anti-EMILIN1 antibody [EPR14678] - N-terminal (ab185953) at 1/50000 dilution

Lane 1:

Human uterus tissue lysate at 20 µg

Lane 2:

Human fetal kidney tissue lysate at 20 µg

Lane 3:

Human colon cancer tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 107 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14678

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EMILIN1 also known as elastin microfibril interface located protein 1 is a glycoprotein that plays a role in the extracellular matrix. The protein has a molecular mass of approximately 120 kDa. It is expressed in several tissues with high levels observed in vascular tissues skin and other connective tissues. EMILIN1 interacts with other matrix proteins which helps maintain the structural integrity of tissues.

Biological function summary

EMILIN1 contributes to maintaining elastogenesis and modulating cell adhesion by interacting with integrins. It forms part of the extracellular matrix where it supports elastin fibers which gives elasticity to tissues. EMILIN1 also influences cell behavior by modulating the signaling pathways through its binding to integrins. The proper function of EMILIN1 is important for tissue elasticity and cell migration.

Pathways

EMILIN1 is integral to the Notch signaling and TGF-β pathways. It interacts with Notch receptors affecting cell differentiation and proliferation. Additionally its interplay with TGF-β signaling regulates extracellular matrix organization and angiogenesis. These connections link EMILIN1 to proteins like TGF-β and Notch receptors highlighting its role in maintaining cellular and tissue homeostasis.

Defects or altered expression of EMILIN1 have been implicated in cardiovascular diseases and skin disorders. It has connections to proteins such as integrins and TGF-β which are also involved in these conditions. In cardiovascular disorders EMILIN1 dysfunction can lead to improper vessel formation and elasticity contributing to hypertension and atherosclerosis. Skin disorders involving EMILIN1 may present as conditions with compromised elastic fiber architecture such as cutis laxa.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity.

See full target information EMILIN1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

BMC oral health 23:203 PubMed37024847

2023

The role of EMILIN-1 in the osteo/odontogenic differentiation of dental pulp stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pingmeng Deng,Jing Huang,Qixuan Zhang,Yuejia Li,Jie Li

American journal of human genetics 109:2230-2252 PubMed36351433

2022

EMILIN1 deficiency causes arterial tortuosity with osteopenia and connects impaired elastogenesis with defective collagen fibrillogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Christin S Adamo,Aude Beyens,Alvise Schiavinato,Douglas R Keene,Sara F Tufa,Matthias Mörgelin,Jürgen Brinckmann,Takako Sasaki,Anja Niehoff,Maren Dreiner,Lore Pottie,Laura Muiño-Mosquera,Elif Yilmaz Gulec,Alper Gezdirici,Paola Braghetta,Paolo Bonaldo,Raimund Wagener,Mats Paulsson,Helen Bornaun,Riet De Rycke,Michiel De Bruyne,Femke Baeke,Walter P Devine,Balram Gangaram,Allison Tam,Meena Balasubramanian,Sian Ellard,Sandra Moore,Sofie Symoens,Joseph Shen,Stacey Cole,Ulrike Schwarze,Kathryn W Holmes,Susan J Hayflick,Wojciech Wiszniewski,Sheela Nampoothiri,Elaine C Davis,Lynn Y Sakai,Gerhard Sengle,Bert Callewaert

Disease markers 2017:9185934 PubMed29158612

2017

Detection of Pathological Changes in the Aorta during Thoracic Aortic Aneurysm Progression on Molecular Level.

Applications

Unspecified application

Species

Unspecified reactive species

Miroslava Rabajdová,Peter Urban,Ivana Špaková,Artemiou Panagiotis,Michaela Ferenčáková,Dušan Rybár,Nikita Bobrov,František Sabol,Mária Mareková

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EMILIN1 antibody [EPR14678] - N-terminal

Immunoprecipitation - Anti-EMILIN1 antibody [EPR14678] - N-terminal (AB185953)

Western blot - Anti-EMILIN1 antibody [EPR14678] - N-terminal (AB185953)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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