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AB153979

Anti-EML2 antibody

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(1 Publication)

Rabbit Polyclonal EML2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EML2 aa 200-450.

View Alternative Names

EMAP2, EMAPL2, EML2, Echinoderm microtubule-associated protein-like 2, EMAP-2, HuEMAP-2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-EML2 antibody (AB153979)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EML2 antibody (AB153979)

Immunofluorescence analysis of methanol-fixed HeLa cells labeling EML2 with ab153979 at 1/200 dilution. Lower panel is costained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EML2 antibody (AB153979)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EML2 antibody (AB153979)

Immunohistochemical analysis of paraffin-embedded Human H441 xenograft tissue labeling EML2 with ab153979 at 1/100 dilution.

Western blot - Anti-EML2 antibody (AB153979)
  • WB

Unknown

Western blot - Anti-EML2 antibody (AB153979)

10% SDS PAGE

All lanes:

Western blot - Anti-EML2 antibody (ab153979) at 1/500 dilution

All lanes:

HCT116 whole cell lysate at 30 µg

Predicted band size: 71 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human EML2 aa 200-450. The exact immunogen used to generate this antibody is proprietary information.

O95834

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The EML2 or Echinoderm Microtubule-associated protein Like 2 functions in the regulation of microtubule dynamics. It's a cytosolic protein and can exhibit a molecular weight around 120 kDa. Researchers find EML2 expression in various tissues notably in brain and testes where it likely plays important roles in cellular structure maintenance. This protein shares structural similarities with other members of the EML family highlighting the significance of its conserved WD40 domain.
Biological function summary

EML2 assists in stabilizing microtubules essential for cellular functions such as mitosis and intracellular transport. It is not known to be part of any complex itself but may interact with other proteins involved in microtubule dynamics. EML2 maintains cellular integrity affecting how cells assume shape and move. Mutations or alterations in EML2 can disrupt normal cellular operations indicating its importance for maintaining normal cell physiology.

Pathways

EML2 involves itself in microtubule-related pathways playing a role in cytoskeleton organization. EML2 works in conjunction with tubulin a primary component of microtubules to influence their stability and function. Another associated pathway is the mitotic spindle organization necessary for accurate chromosomal segregation during cell division. EML2 interaction with these pathways suggests it as an important player in cellular division processes.

EML2 links to neurodegenerative disorders and cancers. Alterations in EML2 affect microtubule dynamics which may contribute to diseases such as Alzheimer's where cellular transport and structure become compromised. In cancer deregulation of EML2 can promote uncontrolled cell division. Interactions between EML2 and proteins like Tau in Alzheimer's and EGFR in certain cancers emphasize its relevance to these disease processes making it a target for therapeutic research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tubulin binding protein that inhibits microtubule nucleation and growth, resulting in shorter microtubules.
See full target information EML2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutical biology 61:37-49 PubMed36573499

2022

Amelioration effects of the soybean lecithin-gallic acid complex on iron-overload-induced oxidative stress and liver damage in C57BL/6J mice.

Applications

Unspecified application

Species

Unspecified reactive species

Caihong Wu,Wenxin Zhang,Feifei Yan,Wenwen Dai,Fang Fang,Yanli Gao,Weiwei Cui
View all publications
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Product promise

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