AB85834

Anti-EML4 antibody

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(2 Publications)

Rabbit Polyclonal EML4 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human EML4 aa 900 to C-terminus.

View Alternative Names

C2orf2, EMAPL4, EML4, Echinoderm microtubule-associated protein-like 4, EMAP-4, Restrictedly overexpressed proliferation-associated protein, Ropp 120

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EML4 antibody (AB85834)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EML4 antibody (AB85834)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling EML4 with ab85834 at 1/1000 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-EML4 antibody (AB85834)

Immunoprecipitation/ Western Blot of ab85834
Lane 1 : ab85834 at 3μg/mg whole cell lysate.
Lane 2 : Control IgG.

ab85834 at 0.4μg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-EML4 antibody (ab85834)

Predicted band size: 109 kDa

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Lab

Western blot - Anti-EML4 antibody (AB85834)

Lanes 1 - 3 : Merged signal (red and green). Green - ab85834 observed at 125 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab85834 was shown to react with EML4 in wild-type HeLa cells in Western blot with loss of signal observed in EML4 CRISPR-Cas9 edited cell line. Wild-type HeLa and EML4 CRISPR-Cas9 edited cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab85834 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-EML4 antibody (ab85834) at 1/2000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

EML4 CRISPR-Cas9 edited HeLa cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Predicted band size: 109 kDa

Observed band size: 125 kDa

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Western blot - Anti-EML4 antibody (AB85834)

All lanes:

Western blot - Anti-EML4 antibody (ab85834) at 0.1 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Predicted band size: 109 kDa

Observed band size: 120 kDa,31 kDa,60 kDa,72 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human EML4 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9HC35

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EML4 also known as Echinoderm Microtubule-Associated Protein-Like 4 is a microtubule-associated protein with a mass of approximately 120 kDa. This protein stabilizes microtubules and influences their organization during cell division. EML4 is widely expressed in various tissues particularly in the brain where it plays important roles in neuronal function. It interacts with microtubule structures to ensure proper chromosome segregation and mitotic spindle formation.

Biological function summary

EML4 facilitates microtubule stability and dynamics which are critical for intracellular transport and cell division. It is part of the EML family and can form complexes with other microtubule-associated proteins. EML4 helps in the regulation of structural arrangements that are important in both mitosis and meiosis processes. Its function in axonal transport becomes essential for proper neuronal development and maintenance.

Pathways

EML4 is involved in the MAPK/ERK and Ras signaling pathways. Within these pathways EML4 interacts with key proteins like KRAS and BRAF contributing to signal transduction that controls cell proliferation and differentiation. The protein's regulatory role in microtubule dynamics positions it as a link between cellular signaling and cytoskeletal organization.

EML4 is most commonly associated with non-small cell lung cancer (NSCLC). A gene fusion between EML4 and ALK (anaplastic lymphoma kinase) leads to the production of an oncogenic protein that drives tumorigenesis. This EML4-ALK fusion protein alters normal cell signaling pathways promoting cancer cell survival and growth. Research also connects EML4 to neurodegenerative diseases due to its important role in microtubule stabilization within neurons.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential for the formation and stability of microtubules (MTs) (PubMed : 16890222, PubMed : 31409757). Required for the organization of the mitotic spindle and for the proper attachment of kinetochores to MTs (PubMed : 25789526). Promotes the recruitment of NUDC to the mitotic spindle for mitotic progression (PubMed : 25789526).

See full target information EML4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 54:102345 PubMed40054123

2025

TTC7A-ALK, a novel ALK fusion variant identified in a patient with metastatic lung adenocarcinoma, exhibits excellent response to crizotinib.

Applications

Unspecified application

Species

Unspecified reactive species

Meijin Huang,Xiangqing Zhu,Wenmang Xu,Jun Zhu,Xin Xun,Bin Su,Hong Chen

Redox biology 58:102544 PubMed36427397

2022

CPT1A promotes anoikis resistance in esophageal squamous cell carcinoma via redox homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Tian,Yunxin Lu,Jinfei Lin,Miao Chen,Huijuan Qiu,Wancui Zhu,Haohui Sun,Jinsheng Huang,Han Yang,Wuguo Deng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EML4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EML4 antibody (AB85834)

Immunoprecipitation - Anti-EML4 antibody (AB85834)

Western blot - Anti-EML4 antibody (AB85834)

Western blot - Anti-EML4 antibody (AB85834)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise