AB202975

Anti-EMP-1 antibody

Name: Anti-EMP-1 antibody (ab202975) | Abcam
Brand: Abcam Limited
SKU: AB202975

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Polyclonal EMP-1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Mouse Emp1 aa 100 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Tmp, Emp1, Epithelial membrane protein 1, EMP-1, Tumor-associated membrane protein

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMP-1 antibody (AB202975)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMP-1 antibody (AB202975)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung carcinoma tissue labeling EMP-1 with ab202975 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMP-1 antibody (AB202975)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling EMP-1 with ab202975 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Lab

Western blot - Anti-EMP-1 antibody (AB202975)

All lanes:

Western blot - Anti-EMP-1 antibody (ab202975) at 1/300 dilution

All lanes:

Mouse lung lysates

Secondary

All lanes:

Conjugated secondary antibody at 1/10000 dilution

Predicted band size: 18 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Mouse Emp1 aa 100 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P47801

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>(or 1/50 - 1/200 using a fluorescent secondary antibody).</p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EMP-1 also known as epithelial membrane protein-1 is a transmembrane protein with a molecular weight of approximately 22 kDa. It is expressed in a variety of tissues including epithelial cells and is highly present in the skin lungs and certain immune cells. EMP-1 belongs to the peripheral myelin protein 22 (PMP22) family sharing structural homology with other tetraspan membrane proteins. The protein serves as a cellular marker for epithelial and some mesenchymal-derived tissues.

Biological function summary

EMP-1 plays a significant role in cell-cell interactions and maintenance of cellular structures. It acts as part of a larger protein complex that contributes to cellular differentiation and tissue integrity. EMP-1 influences cell proliferation and modulates cell morphology which is essential during development and tissue repair. This protein also has a function in signal transduction where it may impact cellular responses to external stress.

Pathways

EMP-1 integrates into multiple signaling pathways such as the MAPK/ERK pathway and the Wnt signaling pathway. These pathways are important for processes like cell growth apoptosis and differentiation. EMP-1 interacts with proteins such as β-catenin and ERK thereby influencing the transcriptional regulation of genes involved in cell survival and growth. The protein’s presence in these pathways highlights its role in cellular physiological adaptations.

EMP-1 has been associated with cancers such as lung cancer and head and neck squamous cell carcinoma. Its expression correlates with tumor progression and metastasis. EMP-1 interacts with proteins like E-cadherin potentially disrupting cell adhesion therefore facilitating cancer cell invasion. Understanding the role of EMP-1 in these diseases could help in developing therapeutic strategies targeting its molecular interactions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information Emp1

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Experimental hematology & oncology 12:77 PubMed37679762

2023

CUL4B-DDB1-COP1-mediated UTX downregulation promotes colorectal cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Dakui Luo,Min Chen,Qingguo Li,Kangjunjie Wang,Kaihua Wang,Junqiang Li,Guoxiang Fu,Zezhi Shan,Qi Liu,Yufei Yang,Lei Liang,Yanlei Ma,Yi Qin,Jun Qin,Daming Gao,Xinxiang Li

Neoplasma 70:58-70 PubMed36637083

2023

Zerumbone combined with gefitinib alleviates lung cancer cell growth through the AKT/STAT3/SLC7A11 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Guo Wang,Da-Lei Li,Rong Fan,Meng-Jun Yan

The International journal of biological markers 36:33-39 PubMed34569869

2021

Serum epithelial membrane protein 1 serves as a feasible biomarker in extrahepatic cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Li,Lang Yan,Hao Xue

The Journal of neuroscience : the official journal of the Society for Neuroscience 41:8644-8667 PubMed34493542

2021

Critical Role of Astrocyte NAD Glycohydrolase in Myelin Injury and Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Monica R Langley,Chan-Il Choi,Thais R Peclat,Yong Guo,Whitney L Simon,Hyesook Yoon,Laurel Kleppe,Claudia F Lucchinetti,Claudia C S Chini,Eduardo N Chini,Isobel A Scarisbrick

Glia 69:2111-2132 PubMed33887067

2021

The thrombin receptor modulates astroglia-neuron trophic coupling and neural repair after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ha Neui Kim,Erin M Triplet,Maja Radulovic,Samantha Bouchal,Laurel S Kleppe,Whitney L Simon,Hyesook Yoon,Isobel A Scarisbrick

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EMP-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMP-1 antibody (AB202975)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMP-1 antibody (AB202975)

Western blot - Anti-EMP-1 antibody (AB202975)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise