AB230445

Anti-EMP-1 antibody - N-terminal

Name: Anti-EMP-1 antibody - N-terminal (ab230445) | Abcam
Brand: Abcam Limited
SKU: AB230445
Price: 605 USD
Availability: InStock

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(10 Publications)

Rabbit Polyclonal EMP-1 antibody. N-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human EMP1 aa 1-100 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

B4B, TMP, EMP1, Epithelial membrane protein 1, EMP-1, CL-20, Protein B4B, Tumor-associated membrane protein

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMP-1 antibody - N-terminal (AB230445)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMP-1 antibody - N-terminal (AB230445)

Formalin-fixed, paraffin-embedded human kidney tissue stained for EMP-1 using ab230445 at 1/75 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMP-1 antibody - N-terminal (AB230445)

Formalin-fixed, paraffin-embedded human adrenal tissue stained for EMP-1 using ab230445 at 1/75 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-EMP-1 antibody - N-terminal (AB230445)

All lanes:

Western blot - Anti-EMP-1 antibody - N-terminal (ab230445) at 1/1000 dilution

All lanes:

K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 35 µg

Predicted band size: 18 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human EMP1 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P54849

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/75", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EMP-1 also known as epithelial membrane protein-1 is a transmembrane protein with a molecular weight of approximately 22 kDa. It is expressed in a variety of tissues including epithelial cells and is highly present in the skin lungs and certain immune cells. EMP-1 belongs to the peripheral myelin protein 22 (PMP22) family sharing structural homology with other tetraspan membrane proteins. The protein serves as a cellular marker for epithelial and some mesenchymal-derived tissues.

Biological function summary

EMP-1 plays a significant role in cell-cell interactions and maintenance of cellular structures. It acts as part of a larger protein complex that contributes to cellular differentiation and tissue integrity. EMP-1 influences cell proliferation and modulates cell morphology which is essential during development and tissue repair. This protein also has a function in signal transduction where it may impact cellular responses to external stress.

Pathways

EMP-1 integrates into multiple signaling pathways such as the MAPK/ERK pathway and the Wnt signaling pathway. These pathways are important for processes like cell growth apoptosis and differentiation. EMP-1 interacts with proteins such as β-catenin and ERK thereby influencing the transcriptional regulation of genes involved in cell survival and growth. The protein’s presence in these pathways highlights its role in cellular physiological adaptations.

EMP-1 has been associated with cancers such as lung cancer and head and neck squamous cell carcinoma. Its expression correlates with tumor progression and metastasis. EMP-1 interacts with proteins like E-cadherin potentially disrupting cell adhesion therefore facilitating cancer cell invasion. Understanding the role of EMP-1 in these diseases could help in developing therapeutic strategies targeting its molecular interactions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information EMP1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:143 PubMed40016223

2025

Tumor cell-derived EMP1 is essential for cancer-associated fibroblast infiltration in tumor microenvironment of triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Wang,Dandan Li,Haixiu Ma,Zengyan Li,Juan Wu,Jinwan Qiao,Jun Liu,Jing Zhao,Ronghua Ma,Lin Tian,Lei Zhang,Jianye Yang,Jianing Wang,Shanshan Qin,Zhanhai Su

Nature communications 16:1206 PubMed39885217

2025

EZH2 serves as a viable therapeutic target for myeloma-induced osteolytic bone destruction.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Liu,Zongwei Li,Rui Chen,Zhihong Fang,Zhiqiang Liu,Huan Liu

Experimental hematology & oncology 12:77 PubMed37679762

2023

CUL4B-DDB1-COP1-mediated UTX downregulation promotes colorectal cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Dakui Luo,Min Chen,Qingguo Li,Kangjunjie Wang,Kaihua Wang,Junqiang Li,Guoxiang Fu,Zezhi Shan,Qi Liu,Yufei Yang,Lei Liang,Yanlei Ma,Yi Qin,Jun Qin,Daming Gao,Xinxiang Li

Cell death & disease 14:386 PubMed37386026

2023

SMYD3 drives the proliferation in gastric cancer cells via reducing EMP1 expression in an H4K20me3-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zeng,Gang Ma,Fenglin Cai,Pengliang Wang,Han Liang,Rupeng Zhang,Jingyu Deng,Yong Liu

Cancer medicine 12:9024-9040 PubMed36708070

2023

Identification of EMP1 as a critical gene for cisplatin resistance in ovarian cancer by using integrated bioinformatics analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Qingsong Zeng,Cunjian Yi,Jinzhi Lu,Xiaowen Wang,Keming Chen,Li Hong

Cells 11: PubMed36359855

2022

Chemerin-Induced Down-Regulation of Placenta-Derived Exosomal miR-140-3p and miR-574-3p Promotes Umbilical Vein Endothelial Cells Proliferation, Migration, and Tube Formation in Gestational Diabetes Mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Lixia Zhang,Qi Wu,Shuqi Zhu,Yibo Tang,Yanmin Chen,Danqing Chen,Zhaoxia Liang

Oncology letters 23:41 PubMed34976153

2022

Knockdown of ETV4 promotes autophagy-dependent apoptosis in GBM cells by reducing the transcriptional activation of EMP1.

Applications

Unspecified application

Species

Unspecified reactive species

Junxiang Wang,Chengfa Sun,Jian Li,Hua Jiang,Yun Qiu,Mingjie Gong

Oncology letters 20:2840-2854 PubMed32782602

2020

High expression of predicts a poor prognosis and correlates with immune infiltrates in bladder urothelial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Lin,Tianwen Zhang,Xin Ye,Hongyu Yang

OncoTargets and therapy 13:2047-2055 PubMed32210572

2020

EMP1 Promotes the Proliferation and Invasion of Ovarian Cancer Cells Through Activating the MAPK Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Liu,Yiling Ding,Yanting Nie,Mengyuan Yang

International journal of molecular sciences 20: PubMed31450568

2019

p53, miR-34a and EMP1-Newly Identified Targets of TFF3 Signaling in Y79 Retinoblastoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maike Busch,Stefan Klein,Jan Große-Kreul,Oliver Scheiner,Klaus Metz,Harald Stephan,Nicole Dünker

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EMP-1 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMP-1 antibody - N-terminal (AB230445)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMP-1 antibody - N-terminal (AB230445)

Western blot - Anti-EMP-1 antibody - N-terminal (AB230445)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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