AB236671

Anti-EMP3 antibody

Name: Anti-EMP3 antibody (ab236671) | Abcam
Brand: Abcam Limited
SKU: AB236671
Price: 525 USD
Availability: InStock

(1 Review)

(5 Publications )

Rabbit Polyclonal EMP3 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EMP3 aa 1-100.

View Alternative Names

YMP, EMP3, Epithelial membrane protein 3, EMP-3, Hematopoietic neural membrane protein 1, Protein YMP, HNMP-1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-EMP3 antibody (AB236671)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EMP3 antibody (AB236671)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling EMP3 using ab236671 at 1/100 dilution in ICC/IF. Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody.

Supplier Data

Western blot - Anti-EMP3 antibody (AB236671)

All lanes:

Western blot - Anti-EMP3 antibody (ab236671) at 1/1000 dilution

All lanes:

Mouse heart tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 18 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EMP3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P54852

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EMP3 also known as epithelial membrane protein 3 is a protein with a mass of 25 kDa. This protein belongs to the PMP22/EMP family and is expressed in various tissues including the brain lung and skeletal muscle. EMP3 features a four-transmembrane domain structure and it plays a critical role in cellular communication and signaling. It localizes primarily to the plasma membrane which suggests its involvement in processes requiring membrane interaction.

Biological function summary

EMP3 impacts cell proliferation and differentiation. It participates in the regulation of cell adhesion and may interact with other proteins to influence cellular morphology. Although not part of a larger well-defined complex EMP3's function seems to support the organization and stability of cell-cell junctions. This role implicates it in developmental processes and the maintenance of tissue architecture.

Pathways

EMP3 interacts within cell communication networks. It associates with the MAPK/ERK pathway a major signaling route involved in cell growth and survival. Additionally EMP3's role in the Wnt signaling pathway links it to key regulatory mechanisms in development. These pathways position EMP3 alongside proteins like beta-catenin and ERK highlighting its involvement in signal transmission and potential modulation of gene expression.

EMP3 has connections to certain cancers and neurodevelopmental conditions. Its altered expression has been observed in glioblastoma where it may influence tumor growth and invasiveness. In such contexts EMP3 may work in concert with proteins such as TP53 impacting cell cycle regulation and apoptosis. Additionally research hints at a role in neurodevelopmental disorders implicating its interaction with signaling molecules and contributing to understanding these complex diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Probably involved in cell proliferation and cell-cell interactions.

See full target information EMP3

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1531420 PubMed40519291

2025

Metformin inhibits the proliferation of hepatocellular carcinoma cells through inducing ferroptosis analyzed by phosphoproteomics.

Applications

Unspecified application

Species

Unspecified reactive species

Jihan Sun,Xi Yu,Yuying Shan,Manyun Dai,Andrea Gaucherot,Caide Lu,Shuqi Mao

Translational cancer research 11:2487-2500 PubMed36093528

2022

CD13 downregulation mediated by ubenimex inhibits autophagy to overcome 5-FU resistance by disturbing the EMP3/FAK/NF-κB pathway in gastric cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Xiu,Qie Guo,Fanjing Jing,Yunyan Shi,Fanbo Jing

Nature cell biology 24:307-315 PubMed35288656

2022

Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Maria C Passarelli,Alexandra M Pinzaru,Hosseinali Asgharian,Maria V Liberti,Søren Heissel,Henrik Molina,Hani Goodarzi,Sohail F Tavazoie

International journal of oncology 59: PubMed34476496

2021

Epithelial membrane protein 3 regulates lung cancer stem cells via the TGF‑β signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yeon-Jee Kahm,Rae-Kwon Kim,Uhee Jung,In-Gyu Kim

Journal of experimental & clinical cancer research : CR 40:160 PubMed33964937

2021

EMP3 mediates glioblastoma-associated macrophage infiltration to drive T cell exclusion.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Chen,Jing Jin,Xin Huang,Fan Wu,Hongguang Huang,Renya Zhan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EMP3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-EMP3 antibody (AB236671)

Western blot - Anti-EMP3 antibody (AB236671)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise