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Rabbit Recombinant Monoclonal F4/80 antibody. Suitable for Flow Cyt, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications.

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Images

Flow Cytometry - Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] (AB254293), expandable thumbnail
  • Western blot - Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] (AB254293), expandable thumbnail
  • Western blot - Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] (AB254293), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] (AB254293), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] (AB254293), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Not recommended
Tested
Tested
Tested
Tested

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
1/16000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

Alternative names

EMR1, TM7LN3, ADGRE1, Adhesion G protein-coupled receptor E1, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor

Recommended products

Rabbit Recombinant Monoclonal F4/80 antibody. Suitable for Flow Cyt, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23225-94
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The EMR1/ADGRE1 (F4/80) is a protein known for its role in cellular adhesion and migration. It is also referred to as EMR1 or ADGRE1 and is a member of the class B seven-span transmembrane receptor family. The protein has a molecular mass of about 90 kDa and primarily expresses on the surface of tissue-resident macrophages. Its expression serves as a marker to identify murine macrophages thereby providing a way to study subsets of immune cells within diverse tissues.

Biological function summary

This protein influences the regulation of immune responses and macrophage actions. It does not appear to work alone; it forms part of a larger receptor complex that participates in interaction with other cellular components modulating biological processes such as phagocytosis and the release of cytokines. These roles position the ADGRE1 macrophage in the immune surveillance of tissues influencing homeostasis and response to injury or infection.

Pathways

ADGRE1/EMR1 (F4/80) takes part in signal transduction pathways related to the innate immune response. It interacts with proteins involved in macrophage activation and cellular signaling such as TLR4 and CD14. These pathways are instrumental in mediating responses to pathogens and tissue damage integrating the ADGRE1 marker in processes that initiate and propagate immune signaling cascades.

Associated diseases and disorders

ADGRE1/EMR1 (F4/80) shows involvement in inflammatory conditions and autoimmune disorders. For example it is relevant to research in diseases like inflammatory bowel disease and rheumatoid arthritis where macrophage-mediated inflammation plays a significant role. In these conditions the protein interacts with cytokines like TNF-alpha linking its expression to clinical manifestations and therapeutic targets. This association makes this protein a potential focal point for understanding and potentially mitigating disease pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry - Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] (ab254293), expandable thumbnail

    Flow Cytometry - Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] (ab254293)

    Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized HEK-293T (transfected with myc tagged EMR1/ADGRE1 construct) cells labelling EMR1/ADGRE1 with ab254293 at 1/500 (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 was used as the secondary antibody.

    Cells were surface stained with isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) or ab254293. Then fixed with 2% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647.

  • Western blot - Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] (ab254293), expandable thumbnail

    Western blot - Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] (ab254293)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 70 seconds.

    All lanes: Western blot - Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] (ab254293) at 1/1000 dilution

    Lane 1: Human colon tissue lysate at 40 µg

    Lane 2: Human stomach tissue lysate at 40 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 98 kDa

    Observed band size: 100 kDa

  • Western blot - Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] (ab254293), expandable thumbnail

    Western blot - Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] (ab254293)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Negative control: Human brain (PMID:17823986).

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] (ab254293) at 1/1000 dilution

    Lane 1: Human spleen tissue lysate at 20 µg

    Lane 2: Human brain tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 98 kDa

    Observed band size: 100 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] (ab254293), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] (ab254293)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293 cells labelling EMR1/ADGRE1 with ab254293 at 1/100 dilution, followed by antibody at 1/1000 dilution (Green). Confocal image showing positive staining in HEK-293 cell line transfected with myc-tagged EMR1/ADGRE1 expression vector. 2233S Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is at 1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] (ab254293), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] (ab254293)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling EMR1/ADGRE1 with ab254293 at 1/16000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in eosinophils of human colon (PMID:17823986).The section was incubated with ab254293 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] (ab254293), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 (F4/80) antibody [EPR23225-94] (ab254293)

    Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling EMR1/ADGRE1 with ab254293 at 1/16000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in eosinophils of human gastric cancer (PMID:17823986).The section was incubated with ab254293 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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