Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648)

Rabbit Recombinant Multiclonal F4/80 antibody. Suitable for WB, IP, Flow Cyt, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (AB323648), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Multiclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IP	Flow Cyt	IHC-P
Human	Tested	Not recommended	Not recommended	Tested
Mouse	Tested	Tested	Tested	Tested
Rat	Tested	Not recommended	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

See full target information Adgre1

Function

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

Targets

ADGRE1

Alternative names

Emr1, Gpf480, Adgre1, Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor

Rabbit Recombinant Multiclonal F4/80 antibody. Suitable for WB, IP, Flow Cyt, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Multiclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Clone number: RM1302
Purification technique: Affinity purification Protein A
Specificity: Unsuitable for human FC, human IP and rat IP.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant multiclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

Western blot - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: brain and skeletal muscle.
The identity of the lower MW band at approximately 25 kDa (in lane 1-4) is unknown.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648) at 1/1000 dilution
Lane 1: Mouse spleen tissue lysate at 20 µg
Lane 2: Mouse liver tissue lysate at 20 µg
Lane 3: Mouse brain tissue lysate at 20 µg
Lane 4: Mouse skeletal muscle tissue lysate at 20 µg
Lane 5: Rat spleen tissue lysate at 20 µg
Lane 6: Rat liver tissue lysate at 20 µg
Lane 7: Rat brain tissue lysate at 20 µg
Lane 8: Rat skeletal muscle tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 90-160 kDa, 36 kDa
Exposure time: 180s
Immunoprecipitation - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648)
EMR1/ADGRE1 (F4/80) was immunoprecipitated from 0.35 mg Mouse lymph node tissue lysate with ab323648 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab323648 at 1/1000 dilution.
Blocking and dilution buffer and concentration: 5% NFDM/TBST
All lanes: Immunoprecipitation - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648) at 1/1000 dilution
Lane 1: Mouse lymph node tissue lysate at 10 µg
Lane 2: ab323648 at 1/30 IP in Mouse lymph node tissue lysate at 10 µg
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab323648 in Mouse lymph node tissue lysate at 10 µg
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 90-160 kDa
Exposure time: 154s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling EMR1/ADGRE1 (F4/80) with ab323648 at 1/2000 (0.237 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: positive staining on immune cells of mouse skeletal muscle.

The section was incubated with ab323648 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648)
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling EMR1/ADGRE1 (F4/80) with ab323648 at 1/2000 (0.237 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: positive staining on immune cells of rat skeletal muscle.

The section was incubated with ab323648 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Flow Cytometry - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648)
Flow cytometric analysis of mouse PBMC cells labelling EMR1/ADGRE1 (F4/80) with ab323648 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
mouse PBMC are co-stained with CD11b conjugated BV510.

Gated on viable cell.
Flow Cytometry - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648)
Flow cytometric analysis of mouse PBMC cells labelling EMR1/ADGRE1 (F4/80) with ab323648 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
mouse PBMC are co-stained with CD3 conjugated Alexa Fluor®647.

Gated on viable cell.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling EMR1/ADGRE1 (F4/80) with ab323648 at 1/500 (0.946 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on Kupffer cells of human liver.

The section was incubated with ab323648 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648)
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling EMR1/ADGRE1 (F4/80) with ab323648 at 1/500 (0.946 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on immune cells of human lung cancer.

The section was incubated with ab323648 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling EMR1/ADGRE1 (F4/80) with ab323648 at 1/2000 (0.237 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse spleen.

The section was incubated with ab323648 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling EMR1/ADGRE1 (F4/80) with ab323648 at 1/2000 (0.237 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat spleen.

The section was incubated with ab323648 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling EMR1/ADGRE1 (F4/80) with ab323648 at 1/500 (0.946 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: no staining on human skeletal muscle.

The section was incubated with ab323648 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: cerebellum and skeletal muscle.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate at 20 µg
Lane 2: Human liver tissue lysate at 20 µg
Lane 3: Human skeletal muscle tissue lysate at 20 µg
Lane 4: Human spleen tissue lysate at 20 µg
Lane 5: Human lymph node tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 120 kDa, 36 kDa
Exposure time: 180s
Western blot - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
F4/80 (ADGRE1) is a glycoprotein of approximately 120~160 kDa and detected as a 70-90 kDa band after treated with Protein Deglycosylation MIX II. (or after Protein Deglycosylation Mix II treatment) (PMID: 8550607)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648) at 1/1000 dilution
Lane 1: Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 15 µg
Lane 2: RAW 264.7 lysate treated with Protein Deglycosylation Mix II at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 70-160 kDa, 36 kDa
Exposure time: 15s
Western blot - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: NIH/3T3 (PMID: 8550607).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-EMR1/ADGRE1 (F4/80) antibody [RM1302] (ab323648) at 1/1000 dilution
Lane 1: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 3: J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 90-160 kDa, 36 kDa
Exposure time: 15s