Rabbit Recombinant Monoclonal EMR2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-EMR2 antibody [EPR29988-1] - BSA and Azide free (Capture) (AB321847), expandable thumbnail](https://content.abcam.com/products/images/emr2-antibody-epr29988-1-bsa-and-azide-free-capture-ab321847--sandwich-elisa-img435729.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-EMR2 antibody [EPR29988-1] - BSA and Azide free (Capture) (AB321847)")
![Sandwich ELISA - Anti-EMR2 antibody [EPR29988-1] - BSA and Azide free (Capture) (AB321847), expandable thumbnail](https://content.abcam.com/products/images/emr2-antibody-epr29988-1-bsa-and-azide-free-capture-ab321847--sandwich-elisa-img435728.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-EMR2 antibody [EPR29988-1] - BSA and Azide free (Capture) (AB321847)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Cell surface receptor that binds to the chondroitin sulfate moiety of glycosaminoglycan chains and promotes cell attachment. Promotes granulocyte chemotaxis, degranulation and adhesion. In macrophages, promotes the release of inflammatory cytokines, including IL8 and TNF. Signals probably through G-proteins. Is a regulator of mast cell degranulation (PubMed:26841242).
Alternative names
Adhesion G protein-coupled receptor E2, EGF-like module receptor 2, EGF-like module-containing mucin-like hormone receptor-like 2
Recommended products
Rabbit Recombinant Monoclonal EMR2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Alternative names
Adhesion G protein-coupled receptor E2, EGF-like module receptor 2, EGF-like module-containing mucin-like hormone receptor-like 2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR29988-1
- Purification technique
Affinity purification Protein A
- Specificity
This antibody does not cross-react with human EMR3 and CD97.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab321847 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human EMR2. The recommended pair for sandwich ELISA is:
Capture: ab321847, Human EMR2 Capture Antibody (unconjugated)
Detector: Anti-EMR2 antibody [EPR29988-32] - BSA and Azide free (Capture/Detector) ab321845, Human EMR2 Detector Antibody (unconjugated)
The reference range value is 39.06-5000 pg/mL.
The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EMR2 also known as epidermal growth factor-like module receptor 2 or CD312 is a member of the class B G protein-coupled receptor family. It is a large receptor with an approximate mass of 220 kDa. EMR2 is mainly expressed on leukocytes especially myeloid cells like monocytes and macrophages. EMR2 consists of an extensive extracellular domain that is involved in ligand binding influencing cell adhesion and signaling processes.
- Biological function summary
EMR2 plays a role in the immune system by facilitating cell-cell interactions and guiding leukocyte trafficking. It forms part of a receptor complex that includes other G protein-coupled receptors. The receptor is essential in the regulation of inflammatory responses helping in the recognition and response to pathogens or tissue damage. EMR2's function contributes to the activation and migration of immune cells to sites of infection or injury.
- Pathways
EMR2 interacts with intracellular signaling cascades such as the MAPK and PI3K pathways that manage cell proliferation survival and immune responses. Through these pathways EMR2 connects to proteins such as Akt and ERK facilitating downstream signaling mechanisms important for immune cell activation. The integration of EMR2 in these pathways positions it as an important player in cellular responses to external stimuli.
- Associated diseases and disorders
Aberrations in EMR2 function or expression relate to chronic inflammatory conditions and autoimmune disorders. Conditions such as rheumatoid arthritis and inflammatory bowel disease show altered EMR2 signaling indicating its role in the pathology of these diseases. In the context of these disorders other proteins like TNF-alpha and IL-6 often interact with EMR2 linking it to pro-inflammatory pathways and the exacerbation of disease symptoms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Sandwich ELISA - Anti-EMR2 antibody [EPR29988-1] - BSA and Azide free (Capture) (ab321847), expandable thumbnail](https://content.abcam.com/products/images/emr2-antibody-epr29988-1-bsa-and-azide-free-capture-ab321847--sandwich-elisa-img435729.jpg "Sandwich ELISA - Anti-EMR2 antibody [EPR29988-1] - BSA and Azide free (Capture) (ab321847)")
Sandwich ELISA - Anti-EMR2 antibody [EPR29988-1] - BSA and Azide free (Capture) (ab321847)
Interpolated concentration of native EMR2 was measured at different sample concentrations. Undiluted sample is as follows: U-937 cell extract (1 mg/mL). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.
![Sandwich ELISA - Anti-EMR2 antibody [EPR29988-1] - BSA and Azide free (Capture) (ab321847), expandable thumbnail](https://content.abcam.com/products/images/emr2-antibody-epr29988-1-bsa-and-azide-free-capture-ab321847--sandwich-elisa-img435728.jpg "Sandwich ELISA - Anti-EMR2 antibody [EPR29988-1] - BSA and Azide free (Capture) (ab321847)")
Sandwich ELISA - Anti-EMR2 antibody [EPR29988-1] - BSA and Azide free (Capture) (ab321847)
Sandwich ELISA with the capture antibody ab321847 dilution at 2 ug/mL and detector antibody Anti-EMR2 antibody [EPR29988-32] - BSA and Azide free (Capture/Detector) ab321845 dilution at 0.5 ug/mL.
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