AB94713

Anti-EMX2 antibody

Name: Anti-EMX2 antibody (ab94713) | Abcam
Brand: Abcam Limited
SKU: AB94713
Price: 475 USD
Availability: InStock
Rating: 5 (4 reviews)

(4 Reviews)

(2 Publications)

Rabbit Polyclonal EMX2 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 2 publications.

View Alternative Names

Homeobox protein EMX2, Empty spiracles homolog 2, Empty spiracles-like protein 2, EMX2

2 Images

Lab

Western blot - Anti-EMX2 antibody (AB94713)

All lanes:

Western blot - Anti-EMX2 antibody (ab94713) at 1 µg/mL

Lane 1:

Mouse Hippocampus Tissue Lysate at 10 µg

Lane 2:

Brain (Mouse) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 28 kDa

Observed band size: 260 kDa,76 kDa

true

Exposure time: 16min

Supplier Data

Western blot - Anti-EMX2 antibody (AB94713)

Blocking buffer : 2% BSA

Gel type : MES

Additional bands : 15 kDa (possible non-specific binding), 75 kDa (possible non-specific binding)

All lanes:

Western blot - Anti-EMX2 antibody (ab94713) at 1 µg/mL

Lane 1:

Human Brain lysate at 20 µg

Lane 2:

Mouse Brain lysate at 20 µg

Lane 3:

NIH 3T3 whole cell lysate at 20 µg

Lane 4:

HepG2 whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EMX2 also known as Empty Spiracles Homeobox 2 functions as a homeobox transcription factor. This protein has a mass of approximately 33 kilodaltons. It is mainly expressed in the developing brain where it plays an important role in brain morphogenesis and neuronal development. EMX2 also presents in other tissues such as the kidney and reproductive organs where it contributes to organogenesis.

Biological function summary

The EMX2 protein influences developmental processes by regulating gene expression essential for cellular differentiation and tissue development. It does not belong to a larger complex but interacts with various transcription factors to exert its regulatory functions. EMX2 modulates important aspects of brain development guiding the patterning and regional specification within the cerebral cortex.

Pathways

EMX2 plays an influential role in the Wnt signaling pathway which is key to developmental processes like cell fate determination proliferation and migration. Additionally EMX2 is involved in the regulation of neural lineage specification through its interaction with other proteins such as PAX6. Together these proteins create a regulatory network that controls neural precursor cell proliferation and differentiation.

EMX2 has implications in neurological disorders. Abnormal expression or mutations in the EMX2 gene can contribute to developmental brain disorders such as schizencephaly which involves abnormal clefts or deep grooves in the cerebral hemispheres. The protein also links to cancer pathways notably in gliomas where dysregulation of EMX2 expression can affect tumor progression. Connections between EMX2 and proteins like GLI1 in the Glioma signaling pathway provide insights into its role in oncogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor, which in cooperation with EMX1, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combination with OTX1/2 to specify cell fates in the developing central nervous system. In the inner ear, it controls the distribution of GPR156 at hair cell boundaries, and regulates the organization of stereociliary bundles in opposite orientations across the line of polarity reversal (LPR).

See full target information EMX2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 15:865858 PubMed35875662

2022

Identification of a Novel Small RNA Encoded in the Mouse Urokinase Receptor uPAR Gene () and Its Molecular Target .

Applications

Unspecified application

Species

Unspecified reactive species

Karina D Rysenkova,Konstantin E Troyanovskiy,Polina S Klimovich,Taisiya R Bulyakova,Ekaterina M Shelomentseva,Anna A Shmakova,Daria Yu Tanygina,Olga I Ivashkina,Konstantin V Anokhin,Maxim N Karagyaur,Maria I Zvereva,Kseniya A Rubina,Vsevolod A Tkachuk,Ekaterina V Semina

Nature cell biology 24:981-995 PubMed35697781

2022

Enhanced cortical neural stem cell identity through short SMAD and WNT inhibition in human cerebral organoids facilitates emergence of outer radial glial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Rosebrock,Sneha Arora,Naresh Mutukula,Rotem Volkman,Elzbieta Gralinska,Anastasios Balaskas,Amèlia Aragonés Hernández,René Buschow,Björn Brändl,Franz-Josef Müller,Peter F Arndt,Martin Vingron,Yechiel Elkabetz

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-EMX2 antibody

Western blot - Anti-EMX2 antibody (AB94713)

Western blot - Anti-EMX2 antibody (AB94713)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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