AB239934

Anti-EN1 / Engrailed 1 antibody [EPR3282] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal EN1 / Engrailed 1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Homeobox protein engrailed-1, Homeobox protein en-1, Hu-En-1, EN1

2 Images

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Western blot - Anti-EN1 / Engrailed 1 antibody [EPR3282] - BSA and Azide free (AB239934)

This data was developed using ab108598, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EN1 / Engrailed 1 antibody [EPR3282] (<a href='/en-us/products/primary-antibodies/en1-engrailed-1-antibody-epr3282-ab108598'>ab108598</a>) at 1/1000 dilution

Lane 1:

U87-MG lysate at 10 µg

Lane 2:

SH-SY5Y lysate at 10 µg

Lane 3:

293T lysate at 10 µg

Lane 4:

Human fetal brain lysate at 10 µg

Predicted band size: 40 kDa

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Supplier Data

Western blot - Anti-EN1 / Engrailed 1 antibody [EPR3282] - BSA and Azide free (AB239934)

This data was developed using ab108598, the same antibody clone in a different buffer formulation. Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-EN1 / Engrailed 1 antibody [EPR3282] (<a href='/en-us/products/primary-antibodies/en1-engrailed-1-antibody-epr3282-ab108598'>ab108598</a>) at 1/10000 dilution

Lane 1:

Human brain lysate at 15 µg

Lane 2:

Mouse brain lysate at 15 µg

Lane 3:

Rat brain lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG

Predicted band size: 40 kDa

Observed band size: 50 kDa

false

Exposure time: 7.75s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3282

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

We have data to suggest no cross-reactivity with EN2/Engrailed 2, please contact us if you require further details/experimental data

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab239934 is the carrier-free version of ab108598.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target EN1 also known as Engrailed 1 is a homeobox-containing transcription factor involved in developmental processes. EN1 protein weighs approximately 40 kDa and is expressed primarily in the developing brain especially in the midbrain and hindbrain regions during embryonic development. It also appears in the skeletal and nervous systems where it regulates specific gene expression patterns. The protein sometimes referred to as EN1 protein plays a significant role in tissue specification and morphogenesis during early developmental stages.

Biological function summary

The Engrailed 1 protein influences pattern formation by controlling the transcription of key developmental genes. Part of several transcriptional complexes it binds to DNA sequences aiding in the specification of positional identities during embryogenesis. EN1 also has significant functions in the maintenance of cell fates and is essential for the differentiation of numerous cell types. Through these processes EN1 ensures proper segmental development and organogenesis.

Pathways

Engrailed 1 integrates into multiple signaling pathways that control cell development including the Wnt and Hedgehog pathways. Within the Wnt pathway EN1 acts alongside proteins like β-catenin to manage cellular responses vital for neuronal and skeletal formation. It also interacts with other transcription factors such as Pax2 in the regulation of pathways linked to neural development.

EN1 has connections to conditions like Parkinson's disease and certain congenital disorders like cerebellar hypoplasia. The dysregulation or mutation of EN1 influences the onset and progression of these diseases. It relates to the serotoninergic and dopaminergic systems reflecting its importance in maintaining normal neurotransmitter functions. In Parkinson's the interaction of EN1 with proteins such as α-synuclein demonstrates its potential impact on neurodegeneration and neuron survival.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for proper formation of the apical ectodermal ridge and correct dorsal-ventral patterning in the limb.

See full target information EN1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

ACS nano 16:12276-12289 PubMed35921522

2022

Genetically Engineered MRI-Trackable Extracellular Vesicles as SARS-CoV-2 Mimetics for Mapping ACE2 Binding .

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Galisova,Jiri Zahradnik,Hyla Allouche-Arnon,Mattia I Morandi,Paula Abou Karam,Michal Fisler,Ori Avinoam,Neta Regev-Rudzki,Gideon Schreiber,Amnon Bar-Shir

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EN1 / Engrailed 1 antibody [EPR3282] - BSA and Azide free

Western blot - Anti-EN1 / Engrailed 1 antibody [EPR3282] - BSA and Azide free (AB239934)

Western blot - Anti-EN1 / Engrailed 1 antibody [EPR3282] - BSA and Azide free (AB239934)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise