AB227167

Anti-EN2 antibody

Name: Anti-EN2 antibody (ab227167) | Abcam
Brand: Abcam Limited
SKU: AB227167
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal EN2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EN2.

View Alternative Names

Homeobox protein engrailed-2, Homeobox protein en-2, Hu-En-2, EN2

2 Images

Supplier Data

Immunoprecipitation - Anti-EN2 antibody (AB227167)

EN2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2 μg of ab227167. Western blot was performed from the immunoprecipitate using ab227167 at 1/1000 dilution. Anti-Rabbit IgG was used as secondary antibody.

Lane 1 : HeLa whole cell lysate 40 μg (Input).

Lane 2 : 2 μg preimmune rabbit IgG instead of ab227167 in HeLa whole cell lysate.

Lane 3 : ab227167 IP in HeLa whole cell lysate.

12% SDS-PAGE.

All lanes:

Immunoprecipitation - Anti-EN2 antibody (ab227167)

Predicted band size: 34 kDa

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Supplier Data

Western blot - Anti-EN2 antibody (AB227167)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-EN2 antibody (ab227167) at 1/10000 dilution

All lanes:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 34 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Recombinant Full Length Protein corresponding to Human EN2.

P19622

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Engrailed-2 often called EN2 functions mechanically as a homeobox-containing transcription factor. Its molecular weight is approximately 30 kDa. EN2 controls gene expression by binding to specific DNA sequences influencing the development of various tissues. This protein predominantly expresses in the central nervous system especially in the mid-hindbrain regions during embryonic development. It also maintains expression in some regions of the adult brain.

Biological function summary

Engrailed-2 plays a significant role in neuronal development and differentiation. It is not a part of a larger complex but acts as a standalone transcription factor to influence the fate of neural progenitor cells. EN2 ensures proper brain patterning and connectivity by regulating genes involved in axon guidance and cell cycle progression. Its expression culminates in the precise formation of neural circuits highlighting its indispensability in neurodevelopment.

Pathways

Engrailed-2 integrates into the Wnt signaling and Sonic Hedgehog pathways which regulate cell differentiation and tissue patterning. In the Wnt pathway EN2 interacts with other homeobox genes to control expression necessary for midbrain development. Through these pathways it shares functional connections with proteins such as Wnt1 and Shh both essential for various stages of embryogenesis and maintaining neuronal homeostasis.

Engrailed-2 associates most strongly with autism spectrum disorders (ASD) and certain types of cancers. Studies link altered EN2 expression and dysfunction to neurodevelopmental anomalies seen in ASD likely due to its critical role in neural patterning. In cancer aberrant EN2 expression levels are noted in prostate cancer with EN2 potentially acting as a diagnostic marker. These dysfunctions implicate EN2 alongside other related proteins like Pax2 in cellular transformation and abnormal growth processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information EN2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer & metabolism 13:24 PubMed40410860

2025

Improved VPS4B O-GlcNAc modification triggers lipid droplets transferring from adipocytes to nasopharyngeal carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Haimeng Yin,Ying Shan,Qin Zhu,Ling Yuan,Feng Ju,Yu Shi,Yumo Han,Rui Wu,Tian Xia,Kaiwen Zhang,Yiwen You,Bo You

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-EN2 antibody

Immunoprecipitation - Anti-EN2 antibody (AB227167)

Western blot - Anti-EN2 antibody (AB227167)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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