AB244417

Anti-ENAH/MENA antibody

Name: Anti-ENAH/MENA antibody (ab244417) | Abcam
Brand: Abcam Limited
SKU: AB244417
Price: 630 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal ENAH/MENA antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ENAH aa 450 to C-terminus.

View Alternative Names

MENA, ENAH, Protein enabled homolog

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody (AB244417)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody (AB244417)

Paraffin-embedded human testis tissue stained for ENAH/MENA using ab244417 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody (AB244417)

Paraffin-embedded human prostate tissue stained for ENAH/MENA using ab244417 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ENAH/MENA antibody (AB244417)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ENAH/MENA antibody (AB244417)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ENAH/MENA (green) using ab244417 at 4 μg/ml in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody (AB244417)

Paraffin-embedded human cerebral cortex tissue stained for ENAH/MENA using ab244417 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody (AB244417)

Paraffin-embedded human liver tissue stained for ENAH/MENA using ab244417 at 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ENAH aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8N8S7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENAH also known as MENA is a protein involved in cell motility and adhesion. Its molecular mass is approximately 80 kDa. This protein is widely expressed in tissues with high rates of cell movement and remodeling like epithelial tissues. ENAH/MENA belongs to the Ena/VASP protein family which is known for its role in regulating the dynamics of actin filaments essential for cell movement processes such as embryogenesis and wound healing.

Biological function summary

ENAH/MENA influences actin filament elongation and organization participating in the structural integrity of the cell cytoskeleton. It works as part of the actin polymerization complex and cooperates with proteins like profilin and VASP. This cooperation ensures proper cell movement and structure maintenance aiding in processes like axonal guidance and immune cell response.

Pathways

ENAH/MENA impacts the regulation of cell migration pathways including the Rho GTPase signaling pathway. It interacts with key signaling molecules like Rac and Cdc42 which coordinate cytoskeletal reorganization. These pathways are important for directional cell migration which is important in normal developmental processes and tissue repair.

ENAH/MENA has been linked to cancer progression particularly in metastasis and neurological disorders such as amyotrophic lateral sclerosis (ALS). In these contexts its interaction with MET receptor and dysregulated signaling can promote invasive cell behavior and neuronal dysfunction highlighting ENAH/MENA as a potential target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation (By similarity).

See full target information ENAH

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed39682720

2024

The Role of the Cytoskeletal Regulatory Protein, Mammalian Enabling Protein (Mena), in Invasion and Metastasis of HPV16-Related Oral Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhichen Guo,Linyang Xie,Hao Cui,Xin Yang,Hong Qi,Ming Yu,Yuxin Gong,Junbo Tu,Sijia Na

Frontiers in oncology 12:1052375 PubMed36620546

2022

Overexpression of Mena is associated with tumor progression and poor prognosis in oral squamous cell carcinoma EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Sijia Na,Hao Cui,Zhichen Guo,Xiang Liang,Karim Ahmed Sakran,Xiaomei Guo,Xingqiang Li,Linyang Xie,Yifei Zhu,Hong Qi,Junbo Tu

International journal of molecular sciences 21: PubMed32599901

2020

Small Nucleolar RNAs Determine Resistance to Doxorubicin in Human Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Godel,Deborah Morena,Preeta Ananthanarayanan,Ilaria Buondonno,Giulio Ferrero,Claudia M Hattinger,Federica Di Nicolantonio,Massimo Serra,Riccardo Taulli,Francesca Cordero,Chiara Riganti,Joanna Kopecka

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ENAH/MENA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody (AB244417)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody (AB244417)

Immunocytochemistry/ Immunofluorescence - Anti-ENAH/MENA antibody (AB244417)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody (AB244417)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody (AB244417)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise