AB244423

Anti-ENAH/MENA antibody

Name: Anti-ENAH/MENA antibody (ab244423) | Abcam
Brand: Abcam Limited
SKU: AB244423
Price: 635 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal ENAH/MENA antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ENAH aa 200-350.

View Alternative Names

MENA, ENAH, Protein enabled homolog

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-ENAH/MENA antibody (AB244423)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ENAH/MENA antibody (AB244423)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ENAH/MENA (green) using ab244423 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody (AB244423)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody (AB244423)

Paraffin-embedded human testis tissue stained for ENAH/MENA using ab244423 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody (AB244423)

Paraffin-embedded human liver tissue stained for ENAH/MENA using ab244423 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody (AB244423)

Paraffin-embedded human cerebral cortex tissue stained for ENAH/MENA using ab244423 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody (AB244423)

Paraffin-embedded human prostate tissue stained for ENAH/MENA using ab244423 at 1/200 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-ENAH/MENA antibody (AB244423)

All lanes:

Western blot - Anti-ENAH/MENA antibody (ab244423) at 0.4 µg/mL

Lane 1:

U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate

Lane 2:

MCF7 (human breast adenocarcinoma cell line) cell lysate

Predicted band size: 67 kDa

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Western blot - Anti-ENAH/MENA antibody (AB244423)

All lanes:

Western blot - Anti-ENAH/MENA antibody (ab244423) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 67 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ENAH aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

Q8N8S7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENAH also known as MENA is a protein involved in cell motility and adhesion. Its molecular mass is approximately 80 kDa. This protein is widely expressed in tissues with high rates of cell movement and remodeling like epithelial tissues. ENAH/MENA belongs to the Ena/VASP protein family which is known for its role in regulating the dynamics of actin filaments essential for cell movement processes such as embryogenesis and wound healing.

Biological function summary

ENAH/MENA influences actin filament elongation and organization participating in the structural integrity of the cell cytoskeleton. It works as part of the actin polymerization complex and cooperates with proteins like profilin and VASP. This cooperation ensures proper cell movement and structure maintenance aiding in processes like axonal guidance and immune cell response.

Pathways

ENAH/MENA impacts the regulation of cell migration pathways including the Rho GTPase signaling pathway. It interacts with key signaling molecules like Rac and Cdc42 which coordinate cytoskeletal reorganization. These pathways are important for directional cell migration which is important in normal developmental processes and tissue repair.

ENAH/MENA has been linked to cancer progression particularly in metastasis and neurological disorders such as amyotrophic lateral sclerosis (ALS). In these contexts its interaction with MET receptor and dysregulated signaling can promote invasive cell behavior and neuronal dysfunction highlighting ENAH/MENA as a potential target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation (By similarity).

See full target information ENAH

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 19:21 PubMed38459605

2024

Mammalian enabled protein enhances tamoxifen sensitivity of the hormone receptor-positive breast cancer patients by suppressing the AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lifang He,Chuanghong She,Sen Jiang,Zhaochang Qi,Zihao Deng,Likeng Ji,Yukun Cui,Jundong Wu

Journal of Cancer 14:1011-1023 PubMed37151390

2023

CRTC2 promotes paclitaxel resistance by inducing autophagy in ovarian cancer in part via the PI3K-AKT signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoyang Ou,Chen Peng,Yilang Wang,Shiyu Lu,Xinli Yu,Qian He,Aiqin He,Li Zhang

Journal of cellular biochemistry 121:3730-3738 PubMed31736156

2019

Circular RNA hsa_circ_0030018 acts as a sponge of miR-599 to aggravate esophageal carcinoma progression by regulating ENAH expression.

Applications

Unspecified application

Species

Unspecified reactive species

Chengxiang Wang,Derong Tang,Hongying Wang,Guodong Hu,Shuo Hu,Lei Li,Bo Min,Yaqin Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ENAH/MENA antibody

Immunocytochemistry/ Immunofluorescence - Anti-ENAH/MENA antibody (AB244423)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody (AB244423)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody (AB244423)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody (AB244423)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody (AB244423)

Western blot - Anti-ENAH/MENA antibody (AB244423)

Western blot - Anti-ENAH/MENA antibody (AB244423)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise