AB124685

Anti-ENAH/MENA antibody [EPR5660]

RabMAb
Recombinant
What is this?

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(7 Publications)

Rabbit Recombinant Monoclonal ENAH/MENA antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

MENA, ENAH, Protein enabled homolog

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody [EPR5660] (AB124685)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody [EPR5660] (AB124685)

ab124685 at 1/500 dilution staining ENAH/MENA in formalin-fixed paraffin-embedded Human uterus tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ENAH/MENA antibody [EPR5660] (AB124685)

All lanes:

Western blot - Anti-ENAH/MENA antibody [EPR5660] (ab124685) at 1/1000 dilution

Lane 1:

MDA-MB-435 (Human ductal carcinoma cell line) cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

HUVEC cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 67 kDa

Observed band size: 80 kDa

false

Carrier free

Anti-ENAH/MENA antibody [EPR5660] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5660

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "Antigen rerieval is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "Antigen rerieval is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENAH also known as MENA is a protein involved in cell motility and adhesion. Its molecular mass is approximately 80 kDa. This protein is widely expressed in tissues with high rates of cell movement and remodeling like epithelial tissues. ENAH/MENA belongs to the Ena/VASP protein family which is known for its role in regulating the dynamics of actin filaments essential for cell movement processes such as embryogenesis and wound healing.

Biological function summary

ENAH/MENA influences actin filament elongation and organization participating in the structural integrity of the cell cytoskeleton. It works as part of the actin polymerization complex and cooperates with proteins like profilin and VASP. This cooperation ensures proper cell movement and structure maintenance aiding in processes like axonal guidance and immune cell response.

Pathways

ENAH/MENA impacts the regulation of cell migration pathways including the Rho GTPase signaling pathway. It interacts with key signaling molecules like Rac and Cdc42 which coordinate cytoskeletal reorganization. These pathways are important for directional cell migration which is important in normal developmental processes and tissue repair.

ENAH/MENA has been linked to cancer progression particularly in metastasis and neurological disorders such as amyotrophic lateral sclerosis (ALS). In these contexts its interaction with MET receptor and dysregulated signaling can promote invasive cell behavior and neuronal dysfunction highlighting ENAH/MENA as a potential target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation (By similarity).

See full target information ENAH

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 20:1798-1814 PubMed38705725

2024

The mammalian actin elongation factor ENAH/MENA contributes to autophagosome formation via its actin regulatory function.

Applications

Unspecified application

Species

Unspecified reactive species

Yueheng Li,Yafei Zhang,Menghui Wang,Junhui Su,Xinjue Dong,Yuqi Yang,Hongshan Wang,QingQuan Li

General physiology and biophysics 43:163-173 PubMed38477606

2024

TRIM67 interacts with ENAH to regulate the apoptosis and autophagy of lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Liu,Kun Miao,Ling Gao,Ling He

Thoracic cancer 14:815-826 PubMed36737402

2023

Circ_0003340 regulates the expression of ENAH to affect the development of esophageal cancer through miR-874-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Wang,Ning Zhao,Shengzu Peng,Tao Zhang

Environmental toxicology 38:522-533 PubMed36336961

2022

LncRNA ZFAS1 promotes laryngeal cancer progression through RBFOX2-mediated MENA alternative splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Baocai Lu,Xiao Li,Wenjie Miao,Qi Liu,Ruixue Li,Can Cui,Qingzu Gao,Rong Lian

Bioengineered 13:2194-2206 PubMed35030977

2022

Enabled homolog (ENAH) regulated by RNA binding protein splicing factor 3b subunit 4 (SF3B4) exacerbates the proliferation, invasion and migration of hepatocellular carcinoma cells via Notch signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guoming Deng,Yufeng Luo,Yaoming Zhang,Jinfeng Zhang,Zongyun He

Journal of experimental & clinical cancer research 39:140 PubMed32698859

2020

Human umbilical cord mesenchymal stem cells-derived exosomes deliver microRNA-375 to downregulate ENAH and thus retard esophageal squamous cell carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhanfeng He,Weihao Li,Tianliang Zheng,Donglei Liu,Song Zhao

Clinical cancer research : an official journal of 18:1598-610 PubMed22322669

2012

Characterization of gene amplification-driven SKP2 overexpression in myxofibrosarcoma: potential implications in tumor progression and therapeutics.

Applications

Unspecified application

Species

Unspecified reactive species

Chien-Feng Li,Ju-Ming Wang,Hong-Yo Kang,Chiung-Kuei Huang,Jun-Wen Wang,Fu-Min Fang,Yu-Hui Wang,Wen-Ren Wu,Shau-Hsuan Li,Shih-Chen Yu,Jen-Chieh Lee,Jui Lan,Yow-Ling Shiue,Li-Ching Wu,Hsuan-Ying Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ENAH/MENA antibody [EPR5660]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody [EPR5660] (AB124685)

Western blot - Anti-ENAH/MENA antibody [EPR5660] (AB124685)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise