AB247959

Anti-ENAH/MENA antibody [EPR5660] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal ENAH/MENA antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MENA, ENAH, Protein enabled homolog

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody [EPR5660] - BSA and Azide free (AB247959)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody [EPR5660] - BSA and Azide free (AB247959)

This data was developed using ab124685, the same antibody clone in a different buffer formulation.

ab124685, at 1/500 dilution, staining ENAH/MENA in formalin-fixed, paraffin-embedded Human uterus tissue, by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ENAH/MENA antibody [EPR5660] - BSA and Azide free (AB247959)

This data was developed using ab124685, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ENAH/MENA antibody [EPR5660] (<a href='/en-us/products/primary-antibodies/enah-mena-antibody-epr5660-ab124685'>ab124685</a>) at 1/1000 dilution

Lane 1:

MDA-MB-435 (Human ductal carcinoma cell line) cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

HUVEC cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 67 kDa

Observed band size: 80 kDa

false

Unconjugated

Anti-ENAH/MENA antibody [EPR5660]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5660

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Antigen retrieval is recommended.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Antigen retrieval is recommended.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab247959 is the carrier-free version of ab124685.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENAH also known as MENA is a protein involved in cell motility and adhesion. Its molecular mass is approximately 80 kDa. This protein is widely expressed in tissues with high rates of cell movement and remodeling like epithelial tissues. ENAH/MENA belongs to the Ena/VASP protein family which is known for its role in regulating the dynamics of actin filaments essential for cell movement processes such as embryogenesis and wound healing.

Biological function summary

ENAH/MENA influences actin filament elongation and organization participating in the structural integrity of the cell cytoskeleton. It works as part of the actin polymerization complex and cooperates with proteins like profilin and VASP. This cooperation ensures proper cell movement and structure maintenance aiding in processes like axonal guidance and immune cell response.

Pathways

ENAH/MENA impacts the regulation of cell migration pathways including the Rho GTPase signaling pathway. It interacts with key signaling molecules like Rac and Cdc42 which coordinate cytoskeletal reorganization. These pathways are important for directional cell migration which is important in normal developmental processes and tissue repair.

ENAH/MENA has been linked to cancer progression particularly in metastasis and neurological disorders such as amyotrophic lateral sclerosis (ALS). In these contexts its interaction with MET receptor and dysregulated signaling can promote invasive cell behavior and neuronal dysfunction highlighting ENAH/MENA as a potential target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation (By similarity).

See full target information ENAH

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Artificial cells, nanomedicine, and biotechnology 48:498-505 PubMed32013579

2020

Long non-coding RNA NKILA weakens TNF-α-induced inflammation of MRC-5 cells by miR-21 up-regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Wang,Jiajie Zhang,Yan Sun,Nan Lv,Jianwei Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ENAH/MENA antibody [EPR5660] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody [EPR5660] - BSA and Azide free (AB247959)

Western blot - Anti-ENAH/MENA antibody [EPR5660] - BSA and Azide free (AB247959)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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