AB247443

Anti-Endo G antibody [EP1665Y] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal Endo G antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.

View Alternative Names

Endo G, ENDOG

2 Images

Unknown

Western blot - Anti-Endo G antibody [EP1665Y] - BSA and Azide free (AB247443)

This data was developed using ab76122, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Endo G antibody [EP1665Y] (<a href='/en-us/products/primary-antibodies/endo-g-antibody-ep1665y-ab76122'>ab76122</a>) at 1/2000 dilution

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/1000 dilution

Predicted band size: 33 kDa

Observed band size: 35 kDa

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Lab

Western blot - Anti-Endo G antibody [EP1665Y] - BSA and Azide free (AB247443)

This data was developed using ab76122, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 cell lysate (20 μg)

Lane 2 : Endo G HAP1 cell lysate (20 μg)

Lane 3 : Mouse liver tissue lysate (20 μg)

Lane 4 : HepG2 cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab76122 observed at 29 kDa. Red - loading control, ab7291, observed at 52 kDa.

ab76122 was shown to specifically react with Endo G when Endo G knockout samples were used. Wild-type and Endo G knockout samples were subjected to SDS-PAGE. ab76122 and ab7291 (loading control to alpha tubulin) were diluted 1/2000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Endo G antibody [EP1665Y] (<a href='/en-us/products/primary-antibodies/endo-g-antibody-ep1665y-ab76122'>ab76122</a>)

Predicted band size: 33 kDa

false

Unconjugated

Anti-Endo G antibody [EP1665Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1665Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Negative results for HepG2/293T (2% PFA 20min fixation).", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "HepG2 cells, no signal detected.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Negative results for HepG2/mouse liver/rat liver/NIH3T3 (2% PFA 20min fixation).", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Negative results for HepG2/293T (2% PFA 20min fixation).", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "HepG2 cells, no signal detected.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Negative results for HepG2/mouse liver/rat liver/NIH3T3 (2% PFA 20min fixation).", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab247443 is the carrier-free version of ab76122.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Endonuclease G (Endo G) is an important mitochondrial nuclease known for its role in DNA and RNA metabolism. Endo G is also referred to as endoglycosidase S and has a molecular mass of approximately 28 kDa. This protein is expressed mainly in the mitochondria but can translocate to the nucleus under certain conditions. It functions by cleaving DNA and RNA which facilitates the processing of nucleic acids in the cell. Endo G exhibits endonuclease activity making it a significant factor in nucleic acid regulation.

Biological function summary

This target plays an essential role in mitochondrial DNA fragmentation during apoptosis. Endo G operates independently of caspases highlighting its role as a non-canonical mediator of apoptosis. It becomes active upon cellular stress usually through the release from the mitochondria where it functions as part of a larger apoptotic complex. This characteristic grants it an important function in programmed cell death mechanisms.

Pathways

Endo G integrates into apoptosis and mitochondrial biogenesis. This protein participates in the intrinsic pathway of apoptosis collaborating with other proteins like apoptosis-inducing factor (AIF) to execute cell death. In the context of mitochondrial biogenesis Endo G involvement suggests potential interaction with proteins that oversee DNA replication and transcription influencing both apoptosis and cellular energy pathways.

Defects or dysregulation in Endo G activity associate with various cancer types and neurodegenerative conditions. In cancer increased Endo G levels correlate with heightened apoptosis emphasizing its potential role in cancer therapy and as a prognostic marker. In neurodegenerative diseases like Parkinson's Endo G may contribute to neuronal cell death interacting with proteins involved in mitochondrial homeostasis. These interactions present insights into therapeutic targets for related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Endonuclease that preferentially catalyzes the cleavage of double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA (PubMed : 25355512). The 5hmC-modified nucleotide does not increase the binding affinity, but instead increases the efficiency of cutting and specifies the site of cleavage for the modified DNAs (By similarity). Shows significantly higher affinity for four-stranded Holliday junction over duplex and single-stranded DNAs (By similarity). Promotes conservative recombination when the DNA is 5hmC-modified (PubMed : 25355512). Promotes autophagy through the suppression of mTOR by its phosphorylation-mediated interaction with YWHAG and its endonuclease activity-mediated DNA damage response (PubMed : 33473107). GSK3-beta mediated phosphorylation of ENDOG enhances its interaction with YWHAG, leading to the release of TSC2 and PIK3C3 from YWHAG resulting in mTOR pathway suppression and autophagy initiation (PubMed : 33473107). Promotes cleavage of mtDNA in response to oxidative and nitrosative stress, in turn inducing compensatory mtDNA replication (PubMed : 29719607).

See full target information Endonuclease G, mitochondrial

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Endo G antibody [EP1665Y] - BSA and Azide free

Western blot - Anti-Endo G antibody [EP1665Y] - BSA and Azide free (AB247443)

Western blot - Anti-Endo G antibody [EP1665Y] - BSA and Azide free (AB247443)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise