Rabbit Recombinant Monoclonal Endo G antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.
Images
![Western blot - Anti-Endo G antibody [EP1665Y] - BSA and Azide free (AB247443), expandable thumbnail](https://content.abcam.com/products/images/endo-g-antibody-ep1665y-bsa-and-azide-free-ab247443--western-blot-img100629.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Endo G antibody [EP1665Y] - BSA and Azide free (AB247443)")
![Western blot - Anti-Endo G antibody [EP1665Y] - BSA and Azide free (AB247443), expandable thumbnail](https://content.abcam.com/products/images/endo-g-antibody-ep1665y-bsa-and-azide-free-ab247443--western-blot-img25205.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Endo G antibody [EP1665Y] - BSA and Azide free (AB247443)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Predicted | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Negative results for HepG2/293T (2% PFA 20min fixation). |
Species Human | Dilution info - | Notes Negative results for HepG2/293T (2% PFA 20min fixation). |
Species Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes HepG2 cells, no signal detected. |
Species Human | Dilution info - | Notes HepG2 cells, no signal detected. |
Species Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Negative results for HepG2/mouse liver/rat liver/NIH3T3 (2% PFA 20min fixation). |
Species Human | Dilution info - | Notes Negative results for HepG2/mouse liver/rat liver/NIH3T3 (2% PFA 20min fixation). |
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Function
Endonuclease that preferentially catalyzes the cleavage of double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA (PubMed:25355512). The 5hmC-modified nucleotide does not increase the binding affinity, but instead increases the efficiency of cutting and specifies the site of cleavage for the modified DNAs (By similarity). Shows significantly higher affinity for four-stranded Holliday junction over duplex and single-stranded DNAs (By similarity). Promotes conservative recombination when the DNA is 5hmC-modified (PubMed:25355512). Promotes autophagy through the suppression of mTOR by its phosphorylation-mediated interaction with YWHAG and its endonuclease activity-mediated DNA damage response (PubMed:33473107). GSK3-beta mediated phosphorylation of ENDOG enhances its interaction with YWHAG, leading to the release of TSC2 and PIK3C3 from YWHAG resulting in mTOR pathway suppression and autophagy initiation (PubMed:33473107). Promotes cleavage of mtDNA in response to oxidative and nitrosative stress, in turn inducing compensatory mtDNA replication (PubMed:29719607).
Alternative names
Endo G, ENDOG
Recommended products
Rabbit Recombinant Monoclonal Endo G antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP1665Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247443 is the carrier-free version of Anti-Endo G antibody [EP1665Y] ab76122.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Endonuclease G (Endo G) is an important mitochondrial nuclease known for its role in DNA and RNA metabolism. Endo G is also referred to as endoglycosidase S and has a molecular mass of approximately 28 kDa. This protein is expressed mainly in the mitochondria but can translocate to the nucleus under certain conditions. It functions by cleaving DNA and RNA which facilitates the processing of nucleic acids in the cell. Endo G exhibits endonuclease activity making it a significant factor in nucleic acid regulation.
- Biological function summary
This target plays an essential role in mitochondrial DNA fragmentation during apoptosis. Endo G operates independently of caspases highlighting its role as a non-canonical mediator of apoptosis. It becomes active upon cellular stress usually through the release from the mitochondria where it functions as part of a larger apoptotic complex. This characteristic grants it an important function in programmed cell death mechanisms.
- Pathways
Endo G integrates into apoptosis and mitochondrial biogenesis. This protein participates in the intrinsic pathway of apoptosis collaborating with other proteins like apoptosis-inducing factor (AIF) to execute cell death. In the context of mitochondrial biogenesis Endo G involvement suggests potential interaction with proteins that oversee DNA replication and transcription influencing both apoptosis and cellular energy pathways.
- Associated diseases and disorders
Defects or dysregulation in Endo G activity associate with various cancer types and neurodegenerative conditions. In cancer increased Endo G levels correlate with heightened apoptosis emphasizing its potential role in cancer therapy and as a prognostic marker. In neurodegenerative diseases like Parkinson's Endo G may contribute to neuronal cell death interacting with proteins involved in mitochondrial homeostasis. These interactions present insights into therapeutic targets for related conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Endo G antibody [EP1665Y] - BSA and Azide free (ab247443), expandable thumbnail](https://content.abcam.com/products/images/endo-g-antibody-ep1665y-bsa-and-azide-free-ab247443--western-blot-img100629.jpg "Western blot - Anti-Endo G antibody [EP1665Y] - BSA and Azide free (ab247443)")
Western blot - Anti-Endo G antibody [EP1665Y] - BSA and Azide free (ab247443)
This data was developed using Anti-Endo G antibody [EP1665Y] ab76122, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Endo G HAP1 cell lysate (20 μg)
Lane 3: Mouse liver tissue lysate (20 μg)
Lane 4: HepG2 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Endo G antibody [EP1665Y] ab76122 observed at 29 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, observed at 52 kDa.
Anti-Endo G antibody [EP1665Y] ab76122 was shown to specifically react with Endo G when Endo G knockout samples were used. Wild-type and Endo G knockout samples were subjected to SDS-PAGE. Anti-Endo G antibody [EP1665Y] ab76122 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (loading control to alpha tubulin) were diluted 1/2000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-Endo G antibody [EP1665Y] (Anti-Endo G antibody [EP1665Y] ab76122)
Predicted band size: 33 kDa
![Western blot - Anti-Endo G antibody [EP1665Y] - BSA and Azide free (ab247443), expandable thumbnail](https://content.abcam.com/products/images/endo-g-antibody-ep1665y-bsa-and-azide-free-ab247443--western-blot-img25205.jpg "Western blot - Anti-Endo G antibody [EP1665Y] - BSA and Azide free (ab247443)")
Western blot - Anti-Endo G antibody [EP1665Y] - BSA and Azide free (ab247443)
This data was developed using Anti-Endo G antibody [EP1665Y] ab76122, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Endo G antibody [EP1665Y] (Anti-Endo G antibody [EP1665Y] ab76122) at 1/2000 dilution
All lanes: HepG2 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 35 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com