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AB121293

Anti-ENDOD1 antibody

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(2 Publications)

Rabbit Polyclonal ENDOD1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ENDOD1 aa 150-350.

View Alternative Names

KIAA0830, ENDOD1, Endonuclease domain-containing 1 protein

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENDOD1 antibody (AB121293)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENDOD1 antibody (AB121293)

ab121293 at 1/1100 dilution staining ENDOD1 in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENDOD1 antibody (AB121293)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENDOD1 antibody (AB121293)

ab121293 at 1/1100 dilution staining ENDOD1 in Paraffin-embedded Human Prostate tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-ENDOD1 antibody (AB121293)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ENDOD1 antibody (AB121293)

Immunocytochemistry/immunofluorescence analysis of human RT4 cell line labelling ENDOD1 cells with ab12193 at 2 μg/mL.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENDOD1 antibody (AB121293)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENDOD1 antibody (AB121293)

ab121293 at 1/1100 dilution staining ENDOD1 in Paraffin-embedded Human pancreas tissue by Immunohistochemistry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENDOD1 antibody (AB121293)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENDOD1 antibody (AB121293)

ab121293 at 1/1100 dilution staining ENDOD1 in Paraffin-embedded Human liver tissue by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ENDOD1 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

O94919

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENDOD1 also known as Endonuclease Domain Containing 1 is a protein with a molecular mass of approximately 63 kDa. This protein exhibits endonuclease activity which means it can cleave phosphodiester bonds within a nucleic acid chain. Researchers have observed its expression in various tissues with notably higher levels in the liver and pancreas. Through its endonuclease function ENDOD1 plays a role in nucleic acid metabolism participating in the processing and breakdown of RNA molecules.
Biological function summary

ENDOD1 is involved in maintaining cellular homeostasis by regulating RNA processing and turnover. It interacts within complex cellular environments contributing to the control of RNA stability. The protein does not operate in isolation but may associate with other nucleases and RNA-binding proteins to effectively mediate its functions. Its activity is important for the control of gene expression via RNA turnover and processing affecting cellular function and health.

Pathways

ENDOD1's role in RNA metabolism ensures its participation in the mRNA surveillance pathway. This pathway monitors the quality of mRNA and eliminates defective transcripts providing an essential quality control mechanism within the cell. ENDOD1 potentially interacts with proteins such as UPF1 a known participant in the nonsense-mediated decay pathway ensuring the degradation of faulty mRNA. Its involvement in these pathways highlights its importance for cellular quality control mechanisms.

ENDOD1 has connections to disorders involving defective RNA metabolism including certain hepatic and digestive diseases. Abnormal expression or mutations in ENDOD1 may disrupt normal RNA processing contributing to disease states. Additionally ENDOD1 has been implicated in liver disease where its interaction with proteins involved in RNA degradation such as XRN1 might play a role in disease pathogenesis. Understanding ENDOD1's function helps in elucidating its role in the development of these diseases aiding in potential therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a DNase and a RNase. Plays a role in the modulation of innate immune signaling through the cGAS-STING pathway by interacting with RNF26.
See full target information ENDOD1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 9: PubMed32614325

2020

Interaction mapping of endoplasmic reticulum ubiquitin ligases identifies modulators of innate immune signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Emma J Fenech,Federica Lari,Philip D Charles,Roman Fischer,Marie Laétitia-Thézénas,Katrin Bagola,Adrienne W Paton,James C Paton,Mads Gyrd-Hansen,Benedikt M Kessler,John C Christianson

BMC cancer 17:360 PubMed28532481

2017

Identification of endonuclease domain-containing 1 as a novel tumor suppressor in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jianguang Qiu,Shubin Peng,Jie Si-Tu,Cheng Hu,Wentao Huang,Yunhua Mao,Wenhan Qiu,Ke Li,Dejuan Wang
View all publications
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