Rabbit Polyclonal Endofin antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZFYVE16 aa 1-300.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ZFYVE16 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z3T8
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Target data
Function
May be involved in regulating membrane trafficking in the endosomal pathway. Overexpression induces endosome aggregation. Required to target TOM1 to endosomes.
Alternative names
Zinc finger FYVE domain-containing protein 16, Endofin, Endosome-associated FYVE domain protein, ZFYVE16, KIAA0305
Recommended products
Rabbit Polyclonal Endofin antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZFYVE16 aa 1-300.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ZFYVE16 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z3T8
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Endofin also known as ZFYVE16 plays a role in cellular signaling processes. This protein contains a FYVE domain which allows it to bind phosphatidylinositol 3-phosphate a lipid that is important for cellular trafficking. Endofin has a mass of approximately 85 kDa. Researchers have found Endofin expressed in various tissues including the brain heart and kidney indicating its widespread functional importance in different biological systems.
- Biological function summary
Endosomes rely on Endofin for maturation and protein sorting. Endofin acts as a scaffold protein within endosomal complexes helping direct the trafficking of proteins within the cell. It interacts with SMAD proteins which play role in TGF-beta signaling and helps mediate signal transduction from the cell surface to the nucleus. Through its interactions Endofin influences important cellular processes like cell growth differentiation and apoptosis.
- Pathways
Endosomal dynamics integrate Endofin into the TGF-beta signaling pathway by its association with SMAD proteins. Endofin modulates the pathway by facilitating the phosphorylation and nuclear translocation of these SMAD proteins. Endofin also relates to the BMP signaling pathway and affects the regulation of cellular responses to developmental signals. Through its pathway integration Endofin regulates both physiological processes and responses to extracellular signals.
- Associated diseases and disorders
Dysfunctions in Endofin can lead to cancer and neurodegenerative diseases. Its involvement in the TGF-beta signaling pathway connects Endofin to tumor progression since defects in this pathway often result in uncontrolled cell proliferation. Furthermore Endofin interacts with Huntington’s disease protein hinting at a potential link to molecular mechanisms underlying neurodegenerative diseases. Understanding Endofin and its pathway connections may aid in developing therapeutic interventions for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-Endofin antibody (ab234801)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Endofin (green) using ab234801 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488 conjugated Goat Anti-Rabbit IgG (H+L).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endofin antibody (ab234801)
Paraffin-embedded human heart tissue stained for Endofin using ab234801 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endofin antibody (ab234801)
Paraffin-embedded human kidney tissue stained for Endofin using ab234801 at 1/100 dilution in immunohistochemical analysis.
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