AB206300

Anti-ENDOGL1/ENGL antibody [EPR15672]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal ENDOGL1/ENGL antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

ENDOGL1, ENDOGL2, ENGL, EXOG, Endonuclease G-like 1, Endo G-like 1

3 Images

Supplier Data

Immunoprecipitation - Anti-ENDOGL1/ENGL antibody [EPR15672] (AB206300)

ENDOGL1/ENGL was immunoprecipitated from 1mg of HUVEC (Human umbilical vein endothelial cell line) whole cell lysate with ab206300 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab206300 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HUVEC whole cell lysate 10ug (Input). Lane 2 : ab206300 IP in HUVEC whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab206300 in HUVEC whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-ENDOGL1/ENGL antibody [EPR15672] (ab206300)

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] (AB206300)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] (ab206300) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Lane 3:

HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] (AB206300)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] (ab206300) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Exposure time: 3min

Carrier free

Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15672

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENDOGL1 also known as Endonuclease G-Like 1 (ENGL) serves as an important enzyme in the cell that cleaves RNA. Its mass is approximately 35 kilodaltons. ENDOGL1 expresses mainly in the mitochondria where it acts closely with other mitochondrial components. This expression is also observed in various tissues throughout the body indicating a broad functional scope. As a ribonuclease it participates in the degradation of RNA which affects the processing and maturation of mitochondrial RNA.

Biological function summary

The role of ENDOGL1 centers around its participation in the maintenance of mitochondrial RNA stability. ENDOGL1 functions within a larger mitochondrial complex contributing to RNA turnover and regulation. By engaging directly with the RNA it plays a significant role in controlling and managing RNA fragments within the mitochondria essential for normal mitochondrial operations. This function ensures the smooth synthesis of the components required for mitochondrial respiration and energy production.

Pathways

ENDOGL1 integrates into mitochondrial RNA decay and processing pathways working alongside key proteins like POLRMT and TFAM involved in mitochondrial transcription and replication. ENDOGL1 influences the integrity and efficiency of these pathways by controlling RNA fragment degradation. Its activity in these pathways supports mitochondrial homeostasis and helps manage the mitochondrial gene expression thereby facilitating various energy production processes such as oxidative phosphorylation.

ENDOGL1 has connections to mitochondrial disorders and certain types of cancers. Disruption in ENDOGL1 function can lead to mitochondrial dysfunctions which contribute to the development of mitochondrial myopathies. Additionally abnormal RNA processing by ENDOGL1 may relate to cancerous transformations as it could affect RNA stability involved in cell growth and division. These pathways position ENDOGL1 as a potential target for therapeutic interventions aiming to address mitochondrial defects and oncology-related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Endo/exonuclease with nicking activity towards supercoiled DNA, a preference for single-stranded DNA and 5'-3' exonuclease activity.

See full target information EXOG

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Translational lung cancer research 14:1351-1370 PubMed40386722

2025

was stabilized through N-methyladenosine methylation and exerted its anticancer effects in lung squamous cell carcinoma by inhibiting SIRT1 activity via CCAR2.

Applications

Unspecified application

Species

Unspecified reactive species

Qihang Yan,Wingshing Wong,Jinsong Lei,Dachuan Liang,Jie Yang,Li Gong,Rossana Berardi,Shuqin Dai,Junye Wang

eLife 8: PubMed31452510

2019

Mutationally-activated PI3'-kinase-α promotes de-differentiation of lung tumors initiated by the BRAF oncoprotein kinase.

Applications

Unspecified application

Species

Unspecified reactive species

J Edward van Veen,Michael Scherzer,Julia Boshuizen,Mollee Chu,Annie Liu,Allison Landman,Shon Green,Christy Trejo,Martin McMahon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ENDOGL1/ENGL antibody [EPR15672]

Immunoprecipitation - Anti-ENDOGL1/ENGL antibody [EPR15672] (AB206300)

Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] (AB206300)

Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] (AB206300)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise