Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (AB251450)

This data was developed using ab206300, the same antibody clone in a different buffer formulation.

ENDOGL1/ENGL was immunoprecipitated from 1mg of HUVEC (Human umbilical vein endothelial cell line) whole cell lysate with ab206300 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab206300 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HUVEC whole cell lysate 10ug (Input). Lane 2 : ab206300 IP in HUVEC whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab206300 in HUVEC whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-ENDOGL1/ENGL antibody [EPR15672] (<a href='/en-us/products/primary-antibodies/endogl1-engl-antibody-epr15672-ab206300'>ab206300</a>)

Predicted band size: 41 kDa

false

• WB

Supplier Data

Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (AB251450)

This data was developed using ab206300, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] (<a href='/en-us/products/primary-antibodies/endogl1-engl-antibody-epr15672-ab206300'>ab206300</a>) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Lane 3:

HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free (AB251450)

This data was developed using ab206300, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ENDOGL1/ENGL antibody [EPR15672] (<a href='/en-us/products/primary-antibodies/endogl1-engl-antibody-epr15672-ab206300'>ab206300</a>) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Exposure time: 3min