AB267424

Anti-Endostatin antibody [EPR22479-286] - BSA and Azide free (Detector)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Endostatin/COL18A1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

View Alternative Names

Collagen alpha-1(XVIII) chain, Col18a1

1 Images

Sandwich ELISA - Anti-Endostatin antibody [EPR22479-286] - BSA and Azide free (Detector) (AB267424)

sELISA

Supplier Data

Sandwich ELISA - Anti-Endostatin antibody [EPR22479-286] - BSA and Azide free (Detector) (AB267424)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab263894).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22479-286

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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Product details

ab267424 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Endostatin. The recommended pair for sandwich ELISA is:
Capture: ab267423, Mouse Endostatin Capture Antibody (unconjugated)
Detector: ab267424, Mouse Endostatin Detector Antibody (unconjugated)
The reference range value is 18.75 - 1200 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Endostatin also known as Collagen XVIII alpha 1 (COL18A1) is a powerful anti-angiogenic protein with a mass around 20 kDa. It specifically inhibits the process of blood vessel formation in both in vitro and in vivo environments. This protein derives as a fragment from the carboxyl end of collagen XVIII which is a component of basement membranes. Expression of endostatin occurs primarily in the endothelial cells lining blood vessels and is also seen in tissues undergoing remodeling or repair.

Biological function summary

Endostatin functions as an important inhibitor of angiogenesis the process in which new blood vessels form from pre-existing ones. It acts independently and is not part of a larger protein complex. In the cellular microenvironment endostatin binds to various integrins inhibiting endothelial cell proliferation and inducing apoptosis. It also disrupts signal transduction pathways critical for endothelial cell survival and migration.

Pathways

Endostatin holds an important position in the angiogenesis and Wnt signaling pathways. It interacts closely with proteins like VEGF (Vascular Endothelial Growth Factor) and FGF2 (Fibroblast Growth Factor 2) where it counteracts their pro-angiogenic effects. Endostatin's role in these pathways indicates its significant impact on controlling angiogenic balance in various physiological and pathological conditions.

Endostatin exhibits notable relevance to cancer and diabetic retinopathy. In cancer the protein suppresses tumor-induced angiogenesis restricting tumor growth and metastasis. Likewise in diabetic retinopathy endostatin's inhibition of abnormal blood vessel formation in the retina offers potential therapeutic benefits. In these contexts it interacts with other key proteins like MMPs (matrix metalloproteinases) which regulate collagen degradation and release of endostatin fragments from parent collagen XVIII.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.. Non-collagenous domain 1. May regulate extracellular matrix-dependent motility and morphogenesis of endothelial and non-endothelial cells; the function requires homotrimerization and implicates MAPK signaling.. Endostatin. Potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling (PubMed : 9008168). Inhibits VEGFA isoform VEGF165-induced endothelial cell proliferation and migration. Seems to inhibit VEGFA-mediated signaling by blocking the interaction of VEGFA to its receptor KDR/VEGFR2 (PubMed : 12029087). Modulates endothelial cell migration in an integrin-dependent manner implicating integrin ITGA5 : ITGB1 and to a lesser extent ITGAV : ITGB3 and ITGAV : ITGB5 (PubMed : 11158588). May negatively regulate the activity of homotrimeric non-collagenous domain 1 (By similarity).

See full target information Col18a1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Endostatin antibody [EPR22479-286] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-Endostatin antibody [EPR22479-286] - BSA and Azide free (Detector) (AB267424)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

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Product promise