AB3453

Anti-Endostatin/COL18A1 antibody

Name: Anti-Endostatin/COL18A1 antibody (ab3453) | Abcam
Brand: Abcam Limited
SKU: AB3453

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(7 Publications)

Rabbit Polyclonal Endostatin/COL18A1 antibody. Suitable for IHC-P, ICC/IF and reacts with Mouse, Human, Primates samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Mouse Col18a1.

View Alternative Names

Collagen alpha-1(XVIII) chain, COL18A1

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-Endostatin/COL18A1 antibody (AB3453)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Endostatin/COL18A1 antibody (AB3453)

ab3453 labelling Endostatin/COL18A1 (green) in A431 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorscence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4 °C. A DyLight 488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei (blue). Images were taken at a magnification of 60x.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endostatin/COL18A1 antibody (AB3453)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endostatin/COL18A1 antibody (AB3453)

ab3453 labelling Endostatin/COL18A1 in the secretion of Human colon tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incuabted with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endostatin/COL18A1 antibody (AB3453)

ab3453 labelling Endostatin/COL18A1 in the secretion of Human kidney tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incuabted with the primary antibody (1 : 100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Endostatin/COL18A1 antibody (AB3453)

ab3453 labelling Endostatin/COL18A1 (green) in 293T cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorscence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4 °C. A DyLight 488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei (blue). Images were taken at a magnification of 60x.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Endostatin/COL18A1 antibody (AB3453)

ab3453 labelling Endostatin/COL18A1 (green) in NIH-3T3 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorscence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4 °C. A DyLight 488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei (blue). Images were taken at a magnification of 60x.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endostatin/COL18A1 antibody (AB3453)

ab3453 labelling Endostatin/COL18A1 in the secretion of Mouse kidney tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incuabted with the primary antibody (1 : 100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Cat, Mouse, Human, Primates

Applications

IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human COL18A1. Database link P39060

Specificity

Detects recombinant human Endostatin/COL18A1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "" }, "Cat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Primates": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "" } } }

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Product details

ab3453 has been recombinant only tested in Western blot.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Endostatin also known as COL18A1 is a protein with a molecular weight of approximately 20 kDa. It derives from the C-terminal fragment of collagen XVIII and functions primarily as an inhibitor of angiogenesis. Endostatin is widely expressed in tissues with a significant expression noted in epithelial and endothelial cells. Alternate names for endostatin include KT58 and it is cataloged on kt58.net. Endostatin interacts with various cell surface receptors and extracellular matrix components to exert its mechanical functions.

Biological function summary

Endostatin modulates cellular processes like migration proliferation and apoptosis. It is involved in the inhibition of endothelial cell proliferation which is an essential function in controlling angiogenesis. Endostatin does not typically form complexes but it has associations with heparan sulfate proteoglycans which facilitates its interaction with cells. Through these interactions endostatin plays a significant role in maintaining vascular homeostasis and tissue remodeling.

Pathways

Endostatin actively participates in the angiogenesis pathway and the regulation of the extracellular matrix pathway. Endostatin's inhibitory effects on angiogenesis involve interactions with pathways associated with key proteins like VEGF and FGF-2 blocking their pro-angiogenic signaling. Endostatin also influences other proteins like integrins which are important for cell adhesion and migration within angiogenesis and matrix remodeling processes.

Endostatin has significant relevance to cancer and ocular disorders. Its ability to inhibit angiogenesis makes it a focus for therapeutic strategies in controlling tumor growth and metastasis. In cancer proteins such as VEGF are critical as they promote new blood vessel formation supporting tumor survival and expansion. In ocular disorders like macular degeneration where abnormal blood vessel growth leads to vision impairment endostatin shows potential in disrupting the detrimental angiogenic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.. Non-collagenous domain 1. May regulate extracellular matrix-dependent motility and morphogenesis of endothelial and non-endothelial cells; the function requires homotrimerization and implicates MAPK signaling.. Endostatin. Potently inhibits endothelial cell proliferation and angiogenesis (PubMed : 9459295). May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling (By similarity). Inhibits VEGFA-induced endothelial cell proliferation and migration. Seems to inhibit VEGFA-mediated signaling by blocking the interaction of VEGFA to its receptor KDR/VEGFR2. Modulates endothelial cell migration in an integrin-dependent manner implicating integrin ITGA5 : ITGB1 and to a lesser extent ITGAV : ITGB3 and ITGAV : ITGB5 (By similarity). May negatively regulate the activity of homotrimeric non-collagenous domain 1 (PubMed : 11257123).

See full target information COL18A1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1188079 PubMed37283766

2023

Basement membrane product, endostatin, as a link between inflammation, coagulation and vascular permeability in COVID-19 and non-COVID-19 acute respiratory distress syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Jandl,Johannes Lorenz Berg,Anna Birnhuber,Elisabeth Fliesser,Izabela Borek,Benjamin Seeliger,Sascha David,Julius J Schmidt,Gregor Gorkiewicz,Martin Zacharias,Tobias Welte,Horst Olschewski,Akos Heinemann,Malgorzata Wygrecka,Grazyna Kwapiszewska

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 25:533-543 PubMed34697264

2021

Triptolide improves myocardial fibrosis in rats through inhibition of nuclear factor kappa B and NLR family pyrin domain containing 3 inflammasome pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianyao Shen,Hailiang Ma,Chaoquan Wang

International journal of molecular medicine 46:2035-2046 PubMed33125088

2020

Resveratrol contributes to the inhibition of liver fibrosis by inducing autophagy via the microRNA‑20a‑mediated activation of the PTEN/PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Zhu,Qiuju Mou,Yinghui Wang,Zixin Zhu,Mingliang Cheng

Blood advances 3:3092-3098 PubMed31648331

2019

Megakaryocytes package contents into separate α-granules that are differentially distributed in platelets.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabeth M Battinelli,Jonathan N Thon,Ross Okazaki,Christian G Peters,Prakrith Vijey,Adrian R Wilkie,Leila J Noetzli,Robert Flaumenhaft,Joseph E Italiano

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 36:295-305 PubMed31598726

2019

Urinary chemokine C-X-C motif ligand 16 and endostatin as predictors of tubulointerstitial fibrosis in patients with advanced diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Ho Lee,Ki Pyo Kim,Sun-Hwa Park,Dong-Jin Kim,Yang-Gyun Kim,Ju-Young Moon,Su-Woong Jung,Jin Sug Kim,Kyung-Hwan Jeong,So-Young Lee,Dong-Ho Yang,Sung-Jig Lim,Jeong-Taek Woo,Sang Youl Rhee,Suk Chon,Hoon-Young Choi,Hyeong-Cheon Park,Young-Il Jo,Joo-Hark Yi,Sang-Woong Han,Sang-Ho Lee

Journal of tissue engineering 10:2041731418820034 PubMed30728936

2019

Selectively activated PRP exerts differential effects on tendon stem/progenitor cells and tendon healing.

Applications

Unspecified application

Species

Unspecified reactive species

Jianying Zhang,Daibang Nie,Kelly Williamson,Jorge L Rocha,MaCalus V Hogan,James H-C Wang

Jundishapur journal of microbiology 9:e34091 PubMed27800134

2016

Effect of Culture Condition Variables on Human Endostatin Gene Expression in Using Response Surface Methodology.

Applications

Unspecified application

Species

Unspecified reactive species

Abbas Mohajeri,Yones Pilehvar-Soltanahmadi,Jalal Abdolalizadeh,Pouran Karimi,Nosratollah Zarghami

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Endostatin/COL18A1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Endostatin/COL18A1 antibody (AB3453)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endostatin/COL18A1 antibody (AB3453)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endostatin/COL18A1 antibody (AB3453)

Immunocytochemistry/ Immunofluorescence - Anti-Endostatin/COL18A1 antibody (AB3453)

Immunocytochemistry/ Immunofluorescence - Anti-Endostatin/COL18A1 antibody (AB3453)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endostatin/COL18A1 antibody (AB3453)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise