AB251463

Anti-Endostatin/COL18A1 antibody [EPR19995] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Endostatin/COL18A1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Collagen alpha-1(XVIII) chain, COL18A1

3 Images

Supplier Data

Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] - BSA and Azide free (AB251463)

This data was developed using ab207162, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Negative control : U937 (PMID : 15451191).

All lanes:

Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] (<a href='/en-us/products/primary-antibodies/endostatin-col18a1-antibody-epr19995-ab207162'>ab207162</a>) at 1/1000 dilution

Lane 1:

Human placenta lysate at 10 µg

Lane 2:

U937 (Human histiocytic lymphoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

Predicted band size: 178 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] - BSA and Azide free (AB251463)

This data was developed using ab207162, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Endostatin and NC1 fragments from proteolytic cleavage are secreted into the culture supernatant as described in the literature (PMID : 15950618).

All lanes:

Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] (<a href='/en-us/products/primary-antibodies/endostatin-col18a1-antibody-epr19995-ab207162'>ab207162</a>) at 1/1000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma cell line) supernatant at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 178 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] - BSA and Azide free (AB251463)

This data was developed using ab207162, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1 and 3 : 1 minute; Lanes 2, 4-6 : 3 minutes.

The MW observed is consistent with the literature (PMID : 10716919; 15451191; 9687493; 11581192; 15545970; 21555590).

All lanes:

Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] (<a href='/en-us/products/primary-antibodies/endostatin-col18a1-antibody-epr19995-ab207162'>ab207162</a>) at 1/1000 dilution

Lane 1:

Human seminoma lysate at 10 µg

Lane 2:

Human testis lysate at 10 µg

Lane 3:

Human fetal liver lysate at 10 µg

Lane 4:

Human fetal heart lysate at 10 µg

Lane 5:

Human fetal kidney lysate at 10 µg

Lane 6:

Human fetal spleen lysate at 10 µg

Secondary

Lanes 1 - 3:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

Lanes 4 - 6:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/50000 dilution

Predicted band size: 178 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19995

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

ab251463 is the carrier-free version of ab207162.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Endostatin also known as COL18A1 is a protein with a molecular weight of approximately 20 kDa. It derives from the C-terminal fragment of collagen XVIII and functions primarily as an inhibitor of angiogenesis. Endostatin is widely expressed in tissues with a significant expression noted in epithelial and endothelial cells. Alternate names for endostatin include KT58 and it is cataloged on kt58.net. Endostatin interacts with various cell surface receptors and extracellular matrix components to exert its mechanical functions.

Biological function summary

Endostatin modulates cellular processes like migration proliferation and apoptosis. It is involved in the inhibition of endothelial cell proliferation which is an essential function in controlling angiogenesis. Endostatin does not typically form complexes but it has associations with heparan sulfate proteoglycans which facilitates its interaction with cells. Through these interactions endostatin plays a significant role in maintaining vascular homeostasis and tissue remodeling.

Pathways

Endostatin actively participates in the angiogenesis pathway and the regulation of the extracellular matrix pathway. Endostatin's inhibitory effects on angiogenesis involve interactions with pathways associated with key proteins like VEGF and FGF-2 blocking their pro-angiogenic signaling. Endostatin also influences other proteins like integrins which are important for cell adhesion and migration within angiogenesis and matrix remodeling processes.

Endostatin has significant relevance to cancer and ocular disorders. Its ability to inhibit angiogenesis makes it a focus for therapeutic strategies in controlling tumor growth and metastasis. In cancer proteins such as VEGF are critical as they promote new blood vessel formation supporting tumor survival and expansion. In ocular disorders like macular degeneration where abnormal blood vessel growth leads to vision impairment endostatin shows potential in disrupting the detrimental angiogenic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.. Non-collagenous domain 1. May regulate extracellular matrix-dependent motility and morphogenesis of endothelial and non-endothelial cells; the function requires homotrimerization and implicates MAPK signaling.. Endostatin. Potently inhibits endothelial cell proliferation and angiogenesis (PubMed : 9459295). May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling (By similarity). Inhibits VEGFA-induced endothelial cell proliferation and migration. Seems to inhibit VEGFA-mediated signaling by blocking the interaction of VEGFA to its receptor KDR/VEGFR2. Modulates endothelial cell migration in an integrin-dependent manner implicating integrin ITGA5 : ITGB1 and to a lesser extent ITGAV : ITGB3 and ITGAV : ITGB5 (By similarity). May negatively regulate the activity of homotrimeric non-collagenous domain 1 (PubMed : 11257123).

See full target information COL18A1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:26521 PubMed39489850

2024

GABPA inhibits tumorigenesis in clear cell renal cell carcinoma by regulating ferroptosis through ACSL4.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqian Wu,Zonglong Wu,Mengfei Yao,Li Liu,Yimeng Song,Lulin Ma,Cheng Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Endostatin/COL18A1 antibody [EPR19995] - BSA and Azide free

Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] - BSA and Azide free (AB251463)

Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] - BSA and Azide free (AB251463)

Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] - BSA and Azide free (AB251463)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise