AB114141

Anti-Endostatin/COL18A1 antibody [KT58]

Name: Anti-Endostatin/COL18A1 antibody [KT58] (ab114141) | Abcam
Brand: Abcam Limited
SKU: AB114141
Price: 535 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Endostatin/COL18A1 antibody. Suitable for sELISA and reacts with Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human COL18A1.

View Alternative Names

Collagen alpha-1(XVIII) chain, COL18A1

1 Images

Sandwich ELISA - Anti-Endostatin/COL18A1 antibody [KT58] (AB114141)

sELISA

Unknown

Sandwich ELISA - Anti-Endostatin/COL18A1 antibody [KT58] (AB114141)

ab114141, capturing Human Endostatin/COL18A1 in a Sandwich ELISA.
ab114141 was used at 5 μg/ml to coat the ELISA plates.
Detection utilised ab106491, which is an IgG2a monoclonal antibody conjugated to HRP.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

KT58

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

sELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human COL18A1.

P39060

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Recombinant full length protein - Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "5 µg/mL", "sELISA-species-notes": "<p>For coating plates.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Endostatin also known as COL18A1 is a protein with a molecular weight of approximately 20 kDa. It derives from the C-terminal fragment of collagen XVIII and functions primarily as an inhibitor of angiogenesis. Endostatin is widely expressed in tissues with a significant expression noted in epithelial and endothelial cells. Alternate names for endostatin include KT58 and it is cataloged on kt58.net. Endostatin interacts with various cell surface receptors and extracellular matrix components to exert its mechanical functions.

Biological function summary

Endostatin modulates cellular processes like migration proliferation and apoptosis. It is involved in the inhibition of endothelial cell proliferation which is an essential function in controlling angiogenesis. Endostatin does not typically form complexes but it has associations with heparan sulfate proteoglycans which facilitates its interaction with cells. Through these interactions endostatin plays a significant role in maintaining vascular homeostasis and tissue remodeling.

Pathways

Endostatin actively participates in the angiogenesis pathway and the regulation of the extracellular matrix pathway. Endostatin's inhibitory effects on angiogenesis involve interactions with pathways associated with key proteins like VEGF and FGF-2 blocking their pro-angiogenic signaling. Endostatin also influences other proteins like integrins which are important for cell adhesion and migration within angiogenesis and matrix remodeling processes.

Endostatin has significant relevance to cancer and ocular disorders. Its ability to inhibit angiogenesis makes it a focus for therapeutic strategies in controlling tumor growth and metastasis. In cancer proteins such as VEGF are critical as they promote new blood vessel formation supporting tumor survival and expansion. In ocular disorders like macular degeneration where abnormal blood vessel growth leads to vision impairment endostatin shows potential in disrupting the detrimental angiogenic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.. Non-collagenous domain 1. May regulate extracellular matrix-dependent motility and morphogenesis of endothelial and non-endothelial cells; the function requires homotrimerization and implicates MAPK signaling.. Endostatin. Potently inhibits endothelial cell proliferation and angiogenesis (PubMed : 9459295). May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling (By similarity). Inhibits VEGFA-induced endothelial cell proliferation and migration. Seems to inhibit VEGFA-mediated signaling by blocking the interaction of VEGFA to its receptor KDR/VEGFR2. Modulates endothelial cell migration in an integrin-dependent manner implicating integrin ITGA5 : ITGB1 and to a lesser extent ITGAV : ITGB3 and ITGAV : ITGB5 (By similarity). May negatively regulate the activity of homotrimeric non-collagenous domain 1 (PubMed : 11257123).

See full target information COL18A1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed39679919

2024

Identification of an early subset of cerebellar nuclei neurons in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Rahimi-Balaei,Shayan Amiri,Thomas Lamonerie,Sih-Rong Wu,Huda Y Zoghbi,G Giacomo Consalez,Daniel Goldowitz,Hassan Marzban

View all publications

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Product promise

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Anti-Endostatin/COL18A1 antibody [KT58]

Sandwich ELISA - Anti-Endostatin/COL18A1 antibody [KT58] (AB114141)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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