Anti-Endothelial Cell antibody [RECA-1] - BSA and Azide free
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(5 Publications)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelial Cell antibody [RECA-1] - BSA and Azide free (AB264524)](https://content.abcam.com/products/images/endothelial-cell-antibody-reca-1-bsa-and-azide-free-ab264524--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img47198.jpg/_jcr_content/renditions/webp-768.webp)
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Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelial Cell antibody [RECA-1] - BSA and Azide free (AB264524)
IHC image of Endothelial Cell staining in Rat brain FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9774, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine and sodium azide (ab9774).
Related conjugates and formulations (2)
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Anti-Endothelial Cell antibody [RECA-1]
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Endothelial Cell antibody [RECA-1]
Complementary Products
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelial Cell antibody [RECA-1] (AB9774)](https://content.abcam.com/products/images/endothelial-cell-antibody-reca-1-ab9774--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img47198.jpg)
Reactivity data
Product details
ab264524 is the carrier-free version of ab9774.
RECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognises a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens.
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This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
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Aliquoting information
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Endothelial cells maintain vascular tone and blood fluidity and they mediate angiogenesis which is the development of new blood vessels from pre-existing ones. They achieve these functions through the expression of adhesion molecules and growth factors. Endothelial cells contribute to the formation of a selective permeability barrier and respond to mechanical and chemical signals. They do not function alone; they are part of a larger vascular complex that includes smooth muscle cells and pericytes.
Pathways
Endothelial cells play key roles in the nitric oxide (NO) signaling and angiogenesis pathways. They produce nitric oxide a critical molecule for vasodilation and blood pressure regulation through the action of enzymes like endothelial nitric oxide synthase (eNOS). In the angiogenesis pathway endothelial cells interact with proteins like vascular endothelial growth factor (VEGF) which signals the proliferation and migration processes necessary for new blood vessel formation.
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Target data
Publications (5)
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Basic research in cardiology 119:699-715 PubMed38963562
2024
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Frontiers in neurology 15:1386654 PubMed38817550
2024
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Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 21:e00306 PubMed38237380
2024
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PeerJ 11:e16358 PubMed38025691
2023
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Cell reports 38:110534 PubMed35320709
2022
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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