AB117757

Anti-Endothelin 1 antibody

Name: Anti-Endothelin 1 antibody (ab117757) | Abcam
Brand: Abcam Limited
SKU: AB117757
Price: 430 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(37 Publications)

Anti-Endothelin 1 antibody (ab117757) is a rabbit polyclonal antibody detecting Endothelin 1 in Western Blot, IHC-P. Suitable for Human, Mouse.

- Over 20 publications

View Alternative Names

Endothelin-1, Preproendothelin-1, PPET1, EDN1

3 Images

Supplier Data

Western blot - Anti-Endothelin 1 antibody (AB117757)

All lanes:

Western blot - Anti-Endothelin 1 antibody (ab117757) at 1/3000 dilution

Lane 1:

HUVEC cell lysate at 30 µg

Lane 2:

HAEC cell lysate at 30 µg

Lane 3:

SK-BR-3 cell lysate at 30 µg

Lane 4:

HEK-293 cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Recombinant Secondary Antibody, HRP at 1/4000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

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Supplier Data

Immunocytochemistry - Anti-Endothelin 1 antibody (AB117757)

Immunocytochemistry analysis of 70% confluent log phase A549 cells labeling Endothelin 1 with ab117757. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab117757 at 1/250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor^® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with SlowFade^® Gold Antifade Mountant with DAPI. F-actin (Panel c : red) was stained with Rhodamine Phalloidin at 1/300. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin 1 antibody (AB117757)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin 1 antibody (AB117757)

ab117757 at 1/2000 dilution staining Endothelin 1 in Mouse Dorsal root ganglia by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC

applications

Immunogen

Synthetic Peptide within Mouse Edn1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P22387

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Endothelin 1 antibody (ab117757) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse samples.

What is the molecular weight of Endothelin 1?
Anti-Endothelin 1 (ab117757) specifically detects a band for Endothelin 1 (UniProt: P05305) at a molecular weight of 24kDa.

Trusted by the scientific community
Anti-Endothelin 1 (ab117757) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.02% Sodium azide Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Endothelin 1 (ET-1) is a potent vasoconstrictor peptide with a molecular mass of approximately 2.5 kDa. The gene encoding ET-1 is expressed predominantly in endothelial cells where it exerts its vasoconstrictive effect. It is synthesized as a prepropeptide that undergoes cleavage to form the mature ET-1 peptide. The primary expression occurs in the vascular endothelial cells but it is also found in the kidney lung and brain indicating its widespread physiological role.

Biological function summary

ET-1 regulates vascular tonus and blood pressure. It is part of the endothelin family that includes endothelin-2 (ET-2) and endothelin-3 (ET-3). ET-1 binds to endothelin receptors such as ETA and ETB which are G protein-coupled receptors facilitating cellular signaling processes. These receptors are abundant in smooth muscle cells and the central nervous system. The interaction of ET-1 with these receptors affects not only vascular smooth muscle contraction but also cell proliferation and production of extracellular matrix proteins.

Pathways

ET-1 plays an important role in the renin-angiotensin-aldosterone system and the nitric oxide signaling pathway. ET-1 interacts closely with angiotensin II a peptide hormone that increases blood pressure by stimulating the production of endothelin in endothelial cells. The release of nitric oxide by endothelium serves as a counter-regulatory mechanism to ET-1-mediated vasoconstriction. The balance between ET-1 and nitric oxide is vital for maintaining vascular homeostasis.

ET-1 is significantly implicated in hypertension and pulmonary arterial hypertension (PAH). The overproduction of ET-1 contributes to sustained high blood pressure and arterial stiffness. In PAH ET-1 levels are increased leading to the constriction and proliferation of pulmonary vascular cells. ET-1's interaction with proteins like transforming growth factor-beta (TGF-Β) further enhances its role in vascular remodeling associated with these conditions. Understanding ET-1's interaction in these pathways has made it a therapeutic target for treating these cardiovascular diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Endothelins are endothelium-derived vasoconstrictor peptides (By similarity). Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells (PubMed : 19086031). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437). Promotes mesenteric arterial wall remodeling via activation of ROCK signaling and subsequent colocalization of NFATC3 with F-actin filaments (By similarity). NFATC3 then translocates to the nucleus where it subsequently promotes the transcription of the smooth muscle hypertrophy and differentiation marker ACTA2 (By similarity).

See full target information EDN1

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Publications (37)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:5626 PubMed39955423

2025

Identification of ECE2 signaling in promoting non-small lung cancer progression through ET1/YAP1/MAGEA3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Huaiqing Xiao,Jiawei Ni,Qun Yu

JCI insight 9: PubMed39316438

2024

GCN2 kinase activation mediates pulmonary vascular remodeling and pulmonary arterial hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Maggie M Zhu,Jingbo Dai,Zhiyu Dai,Yi Peng,You-Yang Zhao

Clinical science (London, England : 1979) 138:1131-1150 PubMed39282930

2024

Identifying mitigating strategies for endothelial cell dysfunction and hypertension in response to VEGF receptor inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas D Camarda,Qing Lu,Dawn M Meola,Joshua J Man,Zeyuan Song,Richard J Travers,Katherine E Lopez,Sarah N Powers,Malvina Papanastasiou,Katherine C DeRuff,James Mullahoo,Shawn B Egri,Desiree Davison,Paola Sebastiani,Scott T Eblen,Rachel Buchsbaum,Gordon S Huggins,Cheryl A London,Jacob D Jaffe,Jenica N Upshaw,Vicky K Yang,Iris Z Jaffe

Nature microbiology 9:2538-2552 PubMed39261580

2024

Blockade of endothelin receptors mitigates SARS-CoV-2-induced osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Man Ting Au,Junguo Ni,Kaiming Tang,Wei Wang,Lanlan Zhang,Hantang Wang,Fangyi Zhao,Zhan Li,Peng Luo,Lawrence Chun-Man Lau,Ping-Keung Chan,Cuiting Luo,Bo Zhou,Lin Zhu,Charlie Yuli Zhang,Tianshu Jiang,Marianne Lauwers,Jasper Fuk-Woo Chan,Shuofeng Yuan,Chunyi Wen

Open life sciences 18:20220735 PubMed37941784

2023

PTX3 promotes IVIG resistance-induced endothelial injury in Kawasaki disease by regulating the NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Sun,Lihua Liu,Ruihua Yang

The Science of the total environment 903:166106 PubMed37567316

2023

Effects of aircraft noise cessation on blood pressure, cardio- and cerebrovascular endothelial function, oxidative stress, and inflammation in an experimental animal model.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Teresa Bayo Jimenez,Adrian Gericke,Katie Frenis,Sanela Rajlic,Miroslava Kvandova,Swenja Kröller-Schön,Matthias Oelze,Marin Kuntic,Ivana Kuntic,Dominika Mihalikova,Qi Tang,Subao Jiang,Yue Ruan,Georg Daniel Duerr,Sebastian Steven,Michael J Schmeisser,Omar Hahad,Huige Li,Andreas Daiber,Thomas Münzel

Cellular and molecular gastroenterology and hepatology 16:513-540 PubMed37336290

2023

Endothelin Receptor-A Inhibition Decreases Ductular Reaction, Liver Fibrosis, and Angiogenesis in a Model of Cholangitis.

Applications

Unspecified application

Species

Unspecified reactive species

Travis Owen,Guido Carpino,Lixian Chen,Debjyoti Kundu,Payton Wills,Burcin Ekser,Paolo Onori,Eugenio Gaudio,Gianfranco Alpini,Heather Francis,Lindsey Kennedy

Ultrasound in medicine & biology 49:1091-1101 PubMed36739244

2023

Low-Intensity Ultrasound Reduces Brain Infarct Size by Upregulating Phosphorylated Endothelial Nitric Oxide in Mouse Model of Middle Cerebral Artery Occlusion.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine M Davis,Azzdine Y Ammi,Wenbin Zhu,Carmen Methner,Zhiping Cao,David Giraud,Nabil J Alkayed,Randy L Woltjer,Sanjiv Kaul

Physiological reports 10:e15505 PubMed36324300

2022

Identification of exercise-regulated genes in mice exposed to cigarette smoke.

Applications

Unspecified application

Species

Unspecified reactive species

Lars Aakerøy,Chew W Cheng,Pavla Sustova,Nathan R Scrimgeour,Sissel Gyrid Freim Wahl,Sigurd Steinshamn,T Scott Bowen,Eivind Brønstad

Nature communications 13:6078 PubMed36241617

2022

Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Weigert,Xiang Zheng,Alina Nenzel,Kati Turkowski,Stefan Günther,Elisabeth Strack,Evelyn Sirait-Fischer,Eiman Elwakeel,Ivan M Kur,Vandana S Nikam,Chanil Valasarajan,Hauke Winter,Alexander Wissgott,Robert Voswinkel,Friedrich Grimminger,Bernhard Brüne,Werner Seeger,Soni Savai Pullamsetti,Rajkumar Savai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Endothelin 1 antibody

Western blot - Anti-Endothelin 1 antibody (AB117757)

Immunocytochemistry - Anti-Endothelin 1 antibody (AB117757)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin 1 antibody (AB117757)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (37)

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