AB97662

Anti-Endothelin 1 antibody

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(1 Publication)

Rabbit Polyclonal Endothelin 1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EDN1 aa 100 to C-terminus.

View Alternative Names

Endothelin-1, Preproendothelin-1, PPET1, EDN1

1 Images

Supplier Data

Western blot - Anti-Endothelin 1 antibody (AB97662)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Endothelin 1 antibody (ab97662) at 1/5000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

EDN1 transfected 293T whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 24 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human EDN1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P05305

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Endothelin 1 (ET-1) is a potent vasoconstrictor peptide with a molecular mass of approximately 2.5 kDa. The gene encoding ET-1 is expressed predominantly in endothelial cells where it exerts its vasoconstrictive effect. It is synthesized as a prepropeptide that undergoes cleavage to form the mature ET-1 peptide. The primary expression occurs in the vascular endothelial cells but it is also found in the kidney lung and brain indicating its widespread physiological role.

Biological function summary

ET-1 regulates vascular tonus and blood pressure. It is part of the endothelin family that includes endothelin-2 (ET-2) and endothelin-3 (ET-3). ET-1 binds to endothelin receptors such as ETA and ETB which are G protein-coupled receptors facilitating cellular signaling processes. These receptors are abundant in smooth muscle cells and the central nervous system. The interaction of ET-1 with these receptors affects not only vascular smooth muscle contraction but also cell proliferation and production of extracellular matrix proteins.

Pathways

ET-1 plays an important role in the renin-angiotensin-aldosterone system and the nitric oxide signaling pathway. ET-1 interacts closely with angiotensin II a peptide hormone that increases blood pressure by stimulating the production of endothelin in endothelial cells. The release of nitric oxide by endothelium serves as a counter-regulatory mechanism to ET-1-mediated vasoconstriction. The balance between ET-1 and nitric oxide is vital for maintaining vascular homeostasis.

ET-1 is significantly implicated in hypertension and pulmonary arterial hypertension (PAH). The overproduction of ET-1 contributes to sustained high blood pressure and arterial stiffness. In PAH ET-1 levels are increased leading to the constriction and proliferation of pulmonary vascular cells. ET-1's interaction with proteins like transforming growth factor-beta (TGF-β) further enhances its role in vascular remodeling associated with these conditions. Understanding ET-1's interaction in these pathways has made it a therapeutic target for treating these cardiovascular diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Endothelins are endothelium-derived vasoconstrictor peptides (By similarity). Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells (PubMed : 19086031). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437). Promotes mesenteric arterial wall remodeling via activation of ROCK signaling and subsequent colocalization of NFATC3 with F-actin filaments (By similarity). NFATC3 then translocates to the nucleus where it subsequently promotes the transcription of the smooth muscle hypertrophy and differentiation marker ACTA2 (By similarity).

See full target information EDN1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2021:9230435 PubMed34777698

2021

miR-4454 Promotes Hepatic Carcinoma Progression by Targeting Vps4A and Rab27A.

Applications

Unspecified application

Species

Unspecified reactive species

Haoming Lin,Rui Zhang,Wenrui Wu,Liming Lei

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Endothelin 1 antibody

Western blot - Anti-Endothelin 1 antibody (AB97662)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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