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Mouse Monoclonal Endothelin 1 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 71 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human EDN1 conjugated to Keyhole Limpet Haemocyanin.
Alternative names=Endothelin-1, Preproendothelin-1, PPET1, EDN1
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Native Full Length Protein corresponding to Human EDN1 conjugated to Keyhole Limpet Haemocyanin. Database link: P05305
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Mouse | Tested | Expected | Expected | Expected |
| Rat | Expected | Tested | Expected | Tested |
Images
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Endothelins are endothelium-derived vasoconstrictor peptides (By similarity). Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells (PubMed:19086031).
Recommended products
Mouse Monoclonal Endothelin 1 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 71 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human EDN1 conjugated to Keyhole Limpet Haemocyanin.
Alternative names=Endothelin-1, Preproendothelin-1, PPET1, EDN1
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Native Full Length Protein corresponding to Human EDN1 conjugated to Keyhole Limpet Haemocyanin. Database link: P05305
- Clone number
TR.ET.48.5
- Purification technique
Affinity purification Protein G
- Specificity
Immunohistochemical staining of ET-1 in human corpus cavernosum tissue with this antibody results in staining of endothelial cells.
Radioimmune assays can be used to concentrate ET-1 in solution (e.g. serum/plasma, milk, urine).- Epitope
Studies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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