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AB246962

Anti-Endothelin 2/ET-2 antibody

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Rabbit Polyclonal Endothelin 2/ET-2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EDN2 aa 50 to C-terminus.

View Alternative Names

Endothelin-2, ET-2, Preproendothelin-2, PPET2, EDN2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Endothelin 2/ET-2 antibody (AB246962)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Endothelin 2/ET-2 antibody (AB246962)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for Endothelin 2/ET-2 (green) using ab246962 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin 2/ET-2 antibody (AB246962)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin 2/ET-2 antibody (AB246962)

Paraffin-embedded human bone marrow tissue stained for Endothelin 2/ET-2 using ab246962 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-Endothelin 2/ET-2 antibody (AB246962)
  • WB

Unknown

Western blot - Anti-Endothelin 2/ET-2 antibody (AB246962)

All lanes:

Western blot - Anti-Endothelin 2/ET-2 antibody (ab246962) at 0.4 mg/mL

Lane 1:

Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

HEK-293T overexpressing Endothelin 2/ET-2 with a C-terminal myc-DDK tag, whole cell lysate.

Predicted band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human EDN2 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P20800

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Endothelin 2 (ET-2) also known as EDN2 is a small peptide with a molecular mass of approximately 2.5 kDa. It belongs to the endothelin family of peptides which includes three isopeptides: ET-1 ET-2 and ET-3. ET-2 expresses in various tissues with notable abundance in the kidney intestines and ovary. The peptide functions by binding to endothelin receptors mainly ETA and ETB on the surface of cells facilitating a range of physiological responses.
Biological function summary

Endothelin 2 modulates vasoconstriction and plays a role in blood pressure regulation. ET-2 functions in tandem with endothelin receptors forming part of a complex signaling network. It influences processes like smooth muscle contraction and cellular proliferation. Additionally ET-2 involves itself in reproductive function particularly within female reproductive tissues affecting ovarian physiology.

Pathways

Endothelin 2 integrates into the endothelin signaling pathway which is pivotal for vascular tone regulation. It interacts with other critical components such as ET-1 within this pathway. ET-2 also relates to the MAPK/ERK pathway which governs cell growth and differentiation. Through these pathways ET-2 coordinates tissue responses to various physiological stimuli often in conjunction with proteins like endothelin receptors and MAPKs.

Endothelin 2 shows a link to conditions such as hypertension and polycystic ovary syndrome (PCOS). Aberrant ET-2 expression can contribute to vascular dysfunction in hypertension. In PCOS ET-2 may regulate ovarian stroma and granulosa cell function with potential connections to proteins like follicle-stimulating hormone (FSH) highlighting its importance in reproductive pathophysiology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Endothelins are endothelium-derived vasoconstrictor peptides.
See full target information EDN2
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Product promise

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