AB117521

Anti-Endothelin A Receptor/ET-A antibody

Name: Anti-Endothelin A Receptor/ET-A antibody (ab117521) | Abcam
Brand: Abcam Limited
SKU: AB117521
Rating: 5 (2 reviews)

(2 Reviews)

(20 Publications)

Rabbit Polyclonal Endothelin A Receptor/ET-A antibody. Suitable for ICC, WB, IHC-P and reacts with Human, Mouse samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Mouse Ednra aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

ETA, ETRA, EDNRA, Endothelin-1 receptor, Endothelin receptor type A, ET-A, ETA-R, hET-AR

4 Images

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Immunocytochemistry - Anti-Endothelin A Receptor/ET-A antibody (AB117521)

ab117521, at 1/5000 dilution, staining Endothelin A Receptor/ET-A in HEK293 transfected with Endothelin A receptor cells by Immunocytochemistry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody (AB117521)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody (AB117521)

ab117251, at 1/2000 dilution, staining Endothelin A Receptor/ET-A in the atrium of mouse heart by Immunohistochemistry.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody (AB117521)

Immunohistochemistry analysis of mouse dorsal root ganglia labeling Endothelin A Receptor/ET-A with ab117521.

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Western blot - Anti-Endothelin A Receptor/ET-A antibody (AB117521)

All lanes:

Western blot - Anti-Endothelin A Receptor/ET-A antibody (ab117521) at 1/5000 dilution

All lanes:

Mouse heart lysate

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC

applications

Immunogen

Synthetic Peptide within Mouse Ednra aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q61614

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

We have received mixed feedback about mouse cross reactivity, some lot works and some doesn't so we no longer guarantee this species.

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 99% Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Endothelin A Receptor (ET-A) also known as endothelin receptor type A is a G protein-coupled receptor responsible for mediating physiological responses to the endothelin family of peptides. It has a molecular weight of approximately 48 kDa. ET-A is expressed in various tissues particularly in vascular smooth muscle cells and certain neural tissues including those resembling ganglioid structures. The engagement of the receptor by endothelin leads to a cascade of intracellular events that result in vasoconstriction and increased blood pressure.

Biological function summary

ET-A functions by binding endothelin peptides specifically endothelin 1 initiating a series of reactions essential for vascular homeostasis. It forms a part of receptor signaling complexes that propagate downstream signals influencing cardiovascular tone and cell proliferation. ET-A holds an important role in modulating secretion of hormones and controlling the nervous system by interacting with signaling molecules.

Pathways

ET-A is heavily involved in the endothelin signaling pathway which interacts with the renin-angiotensin system. Through these pathways it regulates blood pressure and vascular tone. Endothelin receptor type A interfaces with proteins such as angiotensin-converting enzyme influencing vasoconstriction activities and impacting cardiovascular health. These interactions highlight ET-A's important regulatory role in maintaining vascular and cardiac function.

ET-A aligns with conditions like hypertension and pulmonary arterial hypertension. The receptor's overactivity results in elevated vasoconstrictor tone therefore contributing to the mentioned disorders. It associates with proteins like the angiotensin II receptor in these contexts impacting pathological states where vascular balance is disrupted. Understanding the modulation of ET-A offers potential therapeutic strategies for these cardiovascular disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is : ET1 > ET2 >> ET3.

See full target information EDNRA

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 29:e70547 PubMed40245183

2025

Crosstalk Between Gastric Cancer and Adjacent Mucosa Reveals EDN1-EDNRA-Mediated Regulation of Cancer Stemness and Immunomodulation Networks.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobin Zhu,Yating Zhang,Hanlin Liao,Jing Hu,Xiao Xiao

Nature microbiology 9:2538-2552 PubMed39261580

2024

Blockade of endothelin receptors mitigates SARS-CoV-2-induced osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Man Ting Au,Junguo Ni,Kaiming Tang,Wei Wang,Lanlan Zhang,Hantang Wang,Fangyi Zhao,Zhan Li,Peng Luo,Lawrence Chun-Man Lau,Ping-Keung Chan,Cuiting Luo,Bo Zhou,Lin Zhu,Charlie Yuli Zhang,Tianshu Jiang,Marianne Lauwers,Jasper Fuk-Woo Chan,Shuofeng Yuan,Chunyi Wen

International journal of oncology 62: PubMed36453252

2022

The EDN1/EDNRA/β‑arrestin axis promotes colorectal cancer progression by regulating STAT3 phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yeo-Jin Lee,Eunsun Jung,Jinhyeon Choi,Jin-Seong Hwang,Eun-Jeong Jeong,Yuna Roh,Hyun Seung Ban,Sunhong Kim,Seon-Kyu Kim,Seon-Young Kim,Jeong-Ki Min,Tae-Su Han,Jang-Seong Kim

Nature communications 13:6078 PubMed36241617

2022

Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Weigert,Xiang Zheng,Alina Nenzel,Kati Turkowski,Stefan Günther,Elisabeth Strack,Evelyn Sirait-Fischer,Eiman Elwakeel,Ivan M Kur,Vandana S Nikam,Chanil Valasarajan,Hauke Winter,Alexander Wissgott,Robert Voswinkel,Friedrich Grimminger,Bernhard Brüne,Werner Seeger,Soni Savai Pullamsetti,Rajkumar Savai

Diabetes, metabolic syndrome and obesity : targets and therapy 14:963-981 PubMed33688230

2021

Hepatic Knockdown of Endothelin Type A Receptor (ETAR) Ameliorates Hepatic Insulin Resistance and Hyperglycemia Through Suppressing p66Shc-Mediated Mitochondrial Fragmentation in High-Fat Diet-Fed Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Li Feng,Songhua Wang,Feng Chen,Cheng Zhang,Qiao Wang,Yuting Zhao,Zifeng Zhang

Communications biology 3:677 PubMed33188287

2020

Targeting endothelin 1 receptor-miR-200b/c-ZEB1 circuitry blunts metastatic progression in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Rosanna Sestito,Roberta Cianfrocca,Piera Tocci,Laura Rosanò,Andrea Sacconi,Giovanni Blandino,Anna Bagnato

PLoS pathogens 16:e1008947 PubMed33075079

2020

Endothelin receptors promote schistosomiasis-induced hepatic fibrosis via splenic B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Kong,Jinan He,Shusen Guo,Qiqin Song,Dandan Xiang,Ran Tao,Haijing Yu,Guang Chen,Zhiyong Huang,Qin Ning,Jiaquan Huang

Osteoarthritis and cartilage 28:1559-1571 PubMed32858189

2020

Endothelin-1 induces chondrocyte senescence and cartilage damage via endothelin receptor type B in a post-traumatic osteoarthritis mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

M Au,Z Liu,L Rong,Y Zheng,C Wen

Nature communications 11:2138 PubMed32358570

2020

Endothelin-1 signaling maintains glial progenitor proliferation in the postnatal subventricular zone.

Applications

Unspecified application

Species

Unspecified reactive species

Katrina L Adams,Giulia Riparini,Payal Banerjee,Marjolein Breur,Marianna Bugiani,Vittorio Gallo

BMC cardiovascular disorders 20:176 PubMed32295540

2020

Aggravated endothelial endocrine dysfunction and intimal thickening of renal artery in high-fat diet-induced obese pigs following renal denervation.

Applications

Unspecified application

Species

Unspecified reactive species

Enyong Su,Linwei Zhao,Xiaohang Yang,Binbin Zhu,Yahui Liu,Wen Zhao,Xianpei Wang,Datun Qi,Lijie Zhu,Chuanyu Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Endothelin A Receptor/ET-A antibody

Immunocytochemistry - Anti-Endothelin A Receptor/ET-A antibody (AB117521)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody (AB117521)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody (AB117521)

Western blot - Anti-Endothelin A Receptor/ET-A antibody (AB117521)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

Product promise