AB85163

Anti-Endothelin A Receptor/ET-A antibody

Name: Anti-Endothelin A Receptor/ET-A antibody (ab85163) | Abcam
Brand: Abcam Limited
SKU: AB85163
Price: 435 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(25 Publications)

Rabbit Polyclonal Endothelin A Receptor/ET-A antibody. Suitable for WB and reacts with Rat samples. Cited in 25 publications.

View Alternative Names

Endothelin-1 receptor, Endothelin receptor type A, ET-A, ET-AR

2 Images

AbReview19390****

Western blot - Anti-Endothelin A Receptor/ET-A antibody (AB85163)

Blocking step : 5% Milk for 1 hour at 23°C

All lanes:

Western blot - Anti-Endothelin A Receptor/ET-A antibody (ab85163) at 1/250 dilution

All lanes:

Whole tissue lysate of rat lung at 40 µg

Secondary

All lanes:

An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/1000 dilution

Predicted band size: 49 kDa

Observed band size: 48 kDa

true

Exposure time: 30s

This image is courtesy of an Abreview submitted by Nico Jentsch

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Western blot - Anti-Endothelin A Receptor/ET-A antibody (AB85163)

The band observed at 46 kDa could potentially be a cleaved form of Rat Endothelin-1 receptor precursor, as the target protein contains a 20 amino acid signal peptide, which when removed gives an expected molecular weight of 46 kDa.

All lanes:

Western blot - Anti-Endothelin A Receptor/ET-A antibody (ab85163) at 1 µg/mL

Lane 1:

Brain (Rat) Tissue Lysate at 10 µg

Lane 2:

Cerebellum Rat Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 49 kDa

Observed band size: 46 kDa

true

Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Endothelin A Receptor (ET-A) also known as endothelin receptor type A is a G protein-coupled receptor responsible for mediating physiological responses to the endothelin family of peptides. It has a molecular weight of approximately 48 kDa. ET-A is expressed in various tissues particularly in vascular smooth muscle cells and certain neural tissues including those resembling ganglioid structures. The engagement of the receptor by endothelin leads to a cascade of intracellular events that result in vasoconstriction and increased blood pressure.

Biological function summary

ET-A functions by binding endothelin peptides specifically endothelin 1 initiating a series of reactions essential for vascular homeostasis. It forms a part of receptor signaling complexes that propagate downstream signals influencing cardiovascular tone and cell proliferation. ET-A holds an important role in modulating secretion of hormones and controlling the nervous system by interacting with signaling molecules.

Pathways

ET-A is heavily involved in the endothelin signaling pathway which interacts with the renin-angiotensin system. Through these pathways it regulates blood pressure and vascular tone. Endothelin receptor type A interfaces with proteins such as angiotensin-converting enzyme influencing vasoconstriction activities and impacting cardiovascular health. These interactions highlight ET-A's important regulatory role in maintaining vascular and cardiac function.

ET-A aligns with conditions like hypertension and pulmonary arterial hypertension. The receptor's overactivity results in elevated vasoconstrictor tone therefore contributing to the mentioned disorders. It associates with proteins like the angiotensin II receptor in these contexts impacting pathological states where vascular balance is disrupted. Understanding the modulation of ET-A offers potential therapeutic strategies for these cardiovascular disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is : ET1 > ET2 >> ET3.

See full target information Ednra

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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 62: PubMed36453252

2022

The EDN1/EDNRA/β‑arrestin axis promotes colorectal cancer progression by regulating STAT3 phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yeo-Jin Lee,Eunsun Jung,Jinhyeon Choi,Jin-Seong Hwang,Eun-Jeong Jeong,Yuna Roh,Hyun Seung Ban,Sunhong Kim,Seon-Kyu Kim,Seon-Young Kim,Jeong-Ki Min,Tae-Su Han,Jang-Seong Kim

Canadian journal of physiology and pharmacology 100:1087-1096 PubMed36384316

2022

Endothelin A receptors contribute to senescence of brain microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yasir Abdul,Eda Karakaya,Raghavendar Chandran,Sarah Jamil,Adviye Ergul

Journal of thrombosis and thrombolysis 54:411-419 PubMed36006542

2022

MicroRNA-342-3p loaded by human umbilical cord mesenchymal stem cells-derived exosomes attenuates deep vein thrombosis by downregulating EDNRA.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyu Pan,Qian Chen,Hongjian Ding,Huaqing Li

Canadian journal of physiology and pharmacology 100:679-688 PubMed35442801

2022

infection upregulates the endothelin (ET) system in brain microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Eda Karakaya,Yasir Abdul,Nityananda Chowdhury,Bridgette Wellslager,Sarah Jamil,Onder Albayram,Özlem Yilmaz,Adviye Ergul

Journal of inflammation research 15:1209-1226 PubMed35228812

2022

The Involvement of Endothelin Pathway in Chronic Psychological Stress-Induced Bladder Hyperalgesia Through Capsaicin-Sensitive C-Fiber Afferents.

Applications

Unspecified application

Species

Unspecified reactive species

Chuying Qin,Yinhuai Wang,Sai Li,Yuanyuan Tang,Yunliang Gao

Journal of hypertension 40:675-684 PubMed34862331

2021

Rho kinase inhibition ameliorates vascular remodeling and blood pressure elevations in a rat model of apatinib-induced hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Caie Li,Liping Ma,Qiongying Wang,Xuejiao Shao,Lu Guo,Jianshu Chen,Wenjuan Wang,Jing Yu

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 25:159-166 PubMed33602886

2021

NOX4/Src regulates ANP secretion through activating ERK1/2 and Akt/GATA4 signaling in beating rat hypoxic atria.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Zhe Wu,Xiang Li,Lan Hong,Zhuo-Na Han,Ying Liu,Cheng-Xi Wei,Xun Cui

American journal of physiology. Regulatory, integrative and comparative physiology 319:R448-R454 PubMed32813539

2020

Blood pressure and albuminuria in a female mouse model of systemic lupus erythematosus: impact of long-term high salt consumption.

Applications

Unspecified application

Species

Unspecified reactive species

Elena L Dent,Hanna J Broome,Jennifer M Sasser,Michael J Ryan

Lipids in health and disease 19:180 PubMed32758232

2020

Quercetin attenuates reduced uterine perfusion pressure -induced hypertension in pregnant rats through regulation of endothelin-1 and endothelin-1 type A receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Sun,Shuping Zhang,Haitao Song

World journal of gastroenterology 26:2599-2617 PubMed32523314

2020

Sleeve gastrectomy ameliorates endothelial function and prevents lung cancer by normalizing endothelin-1 axis in obese and diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Rexiati Ruze,Ya-Cheng Xiong,Jian-Wen Li,Ming-Wei Zhong,Qian Xu,Zhi-Bo Yan,Jian-Kang Zhu,Yu-Gang Cheng,San-Yuan Hu,Guang-Yong Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Endothelin A Receptor/ET-A antibody

Western blot - Anti-Endothelin A Receptor/ET-A antibody (AB85163)

Western blot - Anti-Endothelin A Receptor/ET-A antibody (AB85163)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (25)

Product promise