AB178454

Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]

Name: Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454) | Abcam
Brand: Abcam Limited
SKU: AB178454
Price: 4865 USD
Availability: InStock
Rating: 4 (1 reviews)

Recombinant
What is this?

(1 Review)

(13 Publications)

Rabbit Recombinant Monoclonal Endothelin A Receptor/ET-A antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 13 publications.

View Alternative Names

ETA, ETRA, EDNRA, Endothelin-1 receptor, Endothelin receptor type A, ET-A, ETA-R, hET-AR

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (AB178454)

IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (AB178454)

Immunohistochemical localization of ETA in human normal lymph node (I) and in neoplastic tissues (A–H) using ab178454. For more protocol information please see PMID : 25708416.

Image generated from hybridoma version of ab178454

Lupp A et al., Peptides, 66, 19-25, Fig 3,; doi: 10.1016/j.peptides.2015.02.005. With permission from Elsevier

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (AB178454)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Endothelin A Receptor/ET-A with ab178454 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (AB178454)

Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Endothelin A Receptor/ET-A with ab178454 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).

Supplier Data

Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (AB178454)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The observed molecular weight is consistent with what has been described in the literature (PMID : 25708416).

All lanes:

Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454) at 1/1000 dilution

Lane 1:

Mouse heart lysate at 10 µg

Lane 2:

Mouse lung lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 49 kDa

Observed band size: 40-70 kDa

true

Exposure time: 26s

Supplier Data

Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (AB178454)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 2:

HEK-293 transfected with Endothelin A Receptor/ET-A (WT) expression vector containing a myc-His-tag® , whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 49 kDa

Observed band size: 40-80 kDa

true

Exposure time: 3min

Carrier free

Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]
578 PE

PE Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]
660 APC

APC Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

UMB-8-37-1

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

This product has switched from a hybridoma to recombinant production method on 9^th September 2020.

The epitope recognized by the UMB-8-37-1 monoclonal contains phosphorylation sites that affect signal in Western blot after treatment with endothelin. Phosphatase treatment may be required prior to Western blot in order to improve signal (PubMed ID 25708416).

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Endothelin A Receptor (ET-A) also known as endothelin receptor type A is a G protein-coupled receptor responsible for mediating physiological responses to the endothelin family of peptides. It has a molecular weight of approximately 48 kDa. ET-A is expressed in various tissues particularly in vascular smooth muscle cells and certain neural tissues including those resembling ganglioid structures. The engagement of the receptor by endothelin leads to a cascade of intracellular events that result in vasoconstriction and increased blood pressure.

Biological function summary

ET-A functions by binding endothelin peptides specifically endothelin 1 initiating a series of reactions essential for vascular homeostasis. It forms a part of receptor signaling complexes that propagate downstream signals influencing cardiovascular tone and cell proliferation. ET-A holds an important role in modulating secretion of hormones and controlling the nervous system by interacting with signaling molecules.

Pathways

ET-A is heavily involved in the endothelin signaling pathway which interacts with the renin-angiotensin system. Through these pathways it regulates blood pressure and vascular tone. Endothelin receptor type A interfaces with proteins such as angiotensin-converting enzyme influencing vasoconstriction activities and impacting cardiovascular health. These interactions highlight ET-A's important regulatory role in maintaining vascular and cardiac function.

ET-A aligns with conditions like hypertension and pulmonary arterial hypertension. The receptor's overactivity results in elevated vasoconstrictor tone therefore contributing to the mentioned disorders. It associates with proteins like the angiotensin II receptor in these contexts impacting pathological states where vascular balance is disrupted. Understanding the modulation of ET-A offers potential therapeutic strategies for these cardiovascular disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is : ET1 > ET2 >> ET3.

See full target information EDNRA

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

BMC cardiovascular disorders 24:11 PubMed38166688

2024

Activation of ETAR and ETBR in myocardial tissue characterizes heart failure induced by experimental autoimmune myocarditis.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Yang,Yujing Wu,Fangfei Li,Jiangfeng Tang,Zhenzhong Zheng,Qingshan Tian

Theranostics 12:5034-5050 PubMed35836807

2022

SGLT2 inhibitor dapagliflozin reduces endothelial dysfunction and microvascular damage during cardiac ischemia/reperfusion injury through normalizing the XO-SERCA2-CaMKII-coffilin pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Li Ma,Rongjun Zou,Wanting Shi,Na Zhou,Shaoxian Chen,Hao Zhou,Xinxin Chen,Yueheng Wu

Cardiovascular diabetology 21:106 PubMed35705980

2022

Empagliflozin attenuates cardiac microvascular ischemia/reperfusion injury through improving mitochondrial homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Rongjun Zou,Wanting Shi,Junxiong Qiu,Na Zhou,Na Du,Hao Zhou,Xinxin Chen,Li Ma

Redox biology 52:102288 PubMed35325804

2022

Empagliflozin attenuates cardiac microvascular ischemia/reperfusion through activating the AMPKα1/ULK1/FUNDC1/mitophagy pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Cai,Zhongzhou Guo,Xing Chang,Ziying Li,Feng Wu,Jing He,Tiantian Cao,Kangrong Wang,Nengxian Shi,Hao Zhou,Sam Toan,David Muid,Ying Tan

Toxicology and applied pharmacology 414:115420 PubMed33503445

2021

Sorafenib not only impairs endothelium-dependent relaxation but also promotes vasoconstriction through the upregulation of vasoconstrictive endothelin type B receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Yu,Kun Li,Andong Zhao,Mengqian Wei,Zhenhao Huang,Yunting Zhang,Ying Chen,Ting Lian,Chuan Wang,Li Xu,Yaping Zhang,Cangbao Xu,Fuqiang Liu

Biomedicines 8: PubMed33255872

2020

Monitoring Endothelin-A Receptor Expression during the Progression of Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Miriam Stölting,Christiane Geyer,Anne Helfen,Anke Hahnenkamp,Marco V Usai,Eva Wardelmann,Michael T Kuhlmann,Moritz Wildgruber,Carsten Höltke

Endocrine-related cancer 27:375-389 PubMed32302973

2020

Molecular profiling for acromegaly treatment: a validation study.

Applications

Unspecified application

Species

Unspecified reactive species

Manel Puig-Domingo,Joan Gil,Miguel Sampedro-Nuñez,Mireia Jordà,Susan M Webb,Guillermo Serra,Laura Pons,Isabel Salinas,Alberto Blanco,Montserrat Marques-Pamies,Elena Valassi,Antonio Picó,Araceli García-Martínez,Cristina Carrato,Raquel Buj,Carlos Del Pozo,Gabriel Obiols,Carles Villabona,Rosa Cámara,Carmen Fajardo-Montañana,Clara V Alvarez,Ignacio Bernabéu,Mónica Marazuela

Molecular pharmaceutics 17:109-117 PubMed31816245

2019

Targeting Endothelin Receptors in a Murine Model of Myocardial Infarction Using a Small Molecular Fluorescent Probe.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie A Kimm,Helena Haas,Miriam Stölting,Michael Kuhlmann,Christiane Geyer,Sarah Glasl,Michael Schäfers,Vasilis Ntziachristos,Moritz Wildgruber,Carsten Höltke

Endocrine-related cancer 25:367-380 PubMed29444910

2018

The neuroendocrine phenotype, genomic profile and therapeutic sensitivity of GEPNET cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Tobias Hofving,Yvonne Arvidsson,Bilal Almobarak,Linda Inge,Roswitha Pfragner,Marta Persson,Göran Stenman,Erik Kristiansson,Viktor Johanson,Ola Nilsson

Oncotarget 8:89958-89969 PubMed29163802

2017

Evaluation of somatostatin, CXCR4 chemokine and endothelin A receptor expression in a large set of paragangliomas.

Applications

IHC-P

Species

Human

Daniel Kaemmerer,Jörg Sänger,Ruza Arsenic,Jan G D'Haese,Jens Neumann,Annette Schmitt-Graeff,Ralph Markus Wirtz,Stefan Schulz,Amelie Lupp

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (AB178454)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (AB178454)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (AB178454)

Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (AB178454)

Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (AB178454)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise