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Rabbit Recombinant Monoclonal Endothelin A Receptor/ET-A antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 9 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (AB178454), expandable thumbnail
  • Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (AB178454), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (AB178454), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (AB178454), expandable thumbnail
  • Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (AB178454), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Tested
Tested
Rat
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3.

Alternative names

ETA, ETRA, EDNRA, Endothelin-1 receptor, Endothelin receptor type A, ET-A, ETA-R, hET-AR

Recommended products

Rabbit Recombinant Monoclonal Endothelin A Receptor/ET-A antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 9 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
UMB-8-37-1
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product has switched from a hybridoma to recombinant production method on 9th September 2020.

The epitope recognized by the UMB-8-37-1 monoclonal contains phosphorylation sites that affect signal in Western blot after treatment with endothelin. Phosphatase treatment may be required prior to Western blot in order to improve signal (PubMed ID 25708416).

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Endothelin A Receptor (ET-A) also known as endothelin receptor type A is a G protein-coupled receptor responsible for mediating physiological responses to the endothelin family of peptides. It has a molecular weight of approximately 48 kDa. ET-A is expressed in various tissues particularly in vascular smooth muscle cells and certain neural tissues including those resembling ganglioid structures. The engagement of the receptor by endothelin leads to a cascade of intracellular events that result in vasoconstriction and increased blood pressure.

Biological function summary

ET-A functions by binding endothelin peptides specifically endothelin 1 initiating a series of reactions essential for vascular homeostasis. It forms a part of receptor signaling complexes that propagate downstream signals influencing cardiovascular tone and cell proliferation. ET-A holds an important role in modulating secretion of hormones and controlling the nervous system by interacting with signaling molecules.

Pathways

ET-A is heavily involved in the endothelin signaling pathway which interacts with the renin-angiotensin system. Through these pathways it regulates blood pressure and vascular tone. Endothelin receptor type A interfaces with proteins such as angiotensin-converting enzyme influencing vasoconstriction activities and impacting cardiovascular health. These interactions highlight ET-A's important regulatory role in maintaining vascular and cardiac function.

Associated diseases and disorders

ET-A aligns with conditions like hypertension and pulmonary arterial hypertension. The receptor's overactivity results in elevated vasoconstrictor tone therefore contributing to the mentioned disorders. It associates with proteins like the angiotensin II receptor in these contexts impacting pathological states where vascular balance is disrupted. Understanding the modulation of ET-A offers potential therapeutic strategies for these cardiovascular disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454), expandable thumbnail
    Lupp A et al., Peptides, 66, 19-25, Fig 3,; doi: 10.1016/j.peptides.2015.02.005. With permission from Elsevier

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

    Immunohistochemical localization of ETA in human normal lymph node (I) and in neoplastic tissues (A–H) using ab178454. For more protocol information please see PMID: 25708416.

    Image generated from hybridoma version of ab178454

  • Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454), expandable thumbnail

    Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The observed molecular weight is consistent with what has been described in the literature (PMID: 25708416).

    All lanes: Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454) at 1/1000 dilution

    Lane 1: Mouse heart lysate at 10 µg

    Lane 2: Mouse lung lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa

    Observed band size: 40-70 kDa

    Exposure time: 26s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Endothelin A Receptor/ET-A with ab178454 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

    Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Endothelin A Receptor/ET-A with ab178454 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).

  • Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454), expandable thumbnail

    Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454) at 1/1000 dilution

    Lane 1: HEK-293 (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 10 µg

    Lane 2: HEK-293 transfected with Endothelin A Receptor/ET-A (WT) expression vector containing a myc-His-tag® , whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa

    Observed band size: 40-80 kDa

    Exposure time: 3min

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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