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AB242440

Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free

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(2 Publications)

Rabbit Recombinant Monoclonal Endothelin A Receptor/ET-A antibody. Carrier free. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 2 publications.

View Alternative Names

ETA, ETRA, EDNRA, Endothelin-1 receptor, Endothelin receptor type A, ET-A, ETA-R, hET-AR

2 Images
Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free (AB242440)
  • WB

Supplier Data

Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free (AB242440)

This data was developed using the same antibody clone in a different buffer formulation (ab178454).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (<a href='/en-us/products/primary-antibodies/endothelin-a-receptor-et-a-antibody-umb-8-37-1-ab178454'>ab178454</a>) at 1/1000 dilution

Lane 1:

Mouse heart lysate at 10 µg

Lane 2:

Mouse lung lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 49 kDa

Observed band size: 40-70 kDa

true

Exposure time: 26s

Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free (AB242440)
  • WB

Supplier Data

Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] - BSA and Azide free (AB242440)

This data was developed using the same antibody clone in a different buffer formulation (ab178454).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (<a href='/en-us/products/primary-antibodies/endothelin-a-receptor-et-a-antibody-umb-8-37-1-ab178454'>ab178454</a>) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 2:

HEK-293 transfected with Endothelin A Receptor/ET-A (WT) expression vector containing a myc-His-tag® , whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 49 kDa

Observed band size: 40-80 kDa

true

Exposure time: 3min

  • Unconjugated

    Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]

  • 660 APC

    APC Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]

  • 578 PE

    PE Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

UMB-8-37-1

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB76259

Anti-Endothelin A Receptor/ET-A antibody

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Primary Antibodies

AB117521

Anti-Endothelin A Receptor/ET-A antibody

Immunocytochemistry - Anti-Endothelin A Receptor/ET-A antibody (AB117521)

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Primary Antibodies

AB85163

Anti-Endothelin A Receptor/ET-A antibody

Western blot - Anti-Endothelin A Receptor/ET-A antibody (AB85163)

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Complementary Products

Primary Antibodies

AB117757

Anti-Endothelin 1 antibody

Western blot - Anti-Endothelin 1 antibody (AB117757)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB117529

Anti-Endothelin B Receptor/ET-B antibody

Immunohistochemistry (Frozen sections) - Anti-Endothelin B Receptor/ET-B antibody (AB117529)

  • 4
  • 4
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

ab242440 is the carrier-free version of ab178454.

This product has switched from a hybridoma to recombinant production method on 9th September 2020.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Endothelin A Receptor (ET-A) also known as endothelin receptor type A is a G protein-coupled receptor responsible for mediating physiological responses to the endothelin family of peptides. It has a molecular weight of approximately 48 kDa. ET-A is expressed in various tissues particularly in vascular smooth muscle cells and certain neural tissues including those resembling ganglioid structures. The engagement of the receptor by endothelin leads to a cascade of intracellular events that result in vasoconstriction and increased blood pressure.
Biological function summary

ET-A functions by binding endothelin peptides specifically endothelin 1 initiating a series of reactions essential for vascular homeostasis. It forms a part of receptor signaling complexes that propagate downstream signals influencing cardiovascular tone and cell proliferation. ET-A holds an important role in modulating secretion of hormones and controlling the nervous system by interacting with signaling molecules.

Pathways

ET-A is heavily involved in the endothelin signaling pathway which interacts with the renin-angiotensin system. Through these pathways it regulates blood pressure and vascular tone. Endothelin receptor type A interfaces with proteins such as angiotensin-converting enzyme influencing vasoconstriction activities and impacting cardiovascular health. These interactions highlight ET-A's important regulatory role in maintaining vascular and cardiac function.

ET-A aligns with conditions like hypertension and pulmonary arterial hypertension. The receptor's overactivity results in elevated vasoconstrictor tone therefore contributing to the mentioned disorders. It associates with proteins like the angiotensin II receptor in these contexts impacting pathological states where vascular balance is disrupted. Understanding the modulation of ET-A offers potential therapeutic strategies for these cardiovascular disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is : ET1 > ET2 >> ET3.
See full target information EDNRA
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:87 PubMed38216744

2024

Protein interaction networks in the vasculature prioritize genes and pathways underlying coronary artery disease.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuyu Martin Zhu,Yu-Han H Hsu,Frederik H Lassen,Bryan T MacDonald,Stephanie Stead,Edyta Malolepsza,April Kim,Taibo Li,Taiji Mizoguchi,Monica Schenone,Gaelen Guzman,Benjamin Tanenbaum,Nadine Fornelos,Steven A Carr,Rajat M Gupta,Patrick T Ellinor,Kasper Lage

Matrix biology : journal of the International Society for Matrix Biology 98:32-48 PubMed34015468

2021

Loss of sphingosine kinase 2 enhances Wilm's tumor suppressor gene 1 and nephrin expression in podocytes and protects from streptozotocin-induced podocytopathy and albuminuria in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Faik Imeri,Bisera Stepanovska Tanturovska,Stephanie Schwalm,Sarbari Saha,Jinyang Zeng-Brouwers,Herrmann Pavenstädt,Josef Pfeilschifter,Liliana Schaefer,Andrea Huwiler
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com