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AB12454

Anti-Engrailed and Invected antibody [4D9]

4

(1 Review)

|

(12 Publications)

Mouse Monoclonal inv antibody. Suitable for IHC - Wmt and reacts with Drosophila melanogaster samples. Cited in 12 publications.

View Alternative Names

CG17835, inv, Homeobox protein invected

3 Images
IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (AB12454)
  • IHC - Wmt

Characteriser16673****

IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (AB12454)

ab12454 used in immunofluorescence at 1/200 on Drosophila embryos.

Briefly, embryos were bleached (5% Bleach) to dechorionate them, followed by fixation in PBS, 3.7% formaldehyde and n-Heptane, dehydration with Methanol, rehydration with PBT (PBS with 0.3% Tritone added) before antibody staining.

This image is courtesy of Dr Torsten Bossing at the University of Plymouth.

IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (AB12454)
  • IHC - Wmt

Collaborator16673****

IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (AB12454)

ab12454 used in immunohistochemistry (Wholemount) at 1/200 dilution on Drosophila embryos.

Briefly, embryos were bleached (5% Bleach) to dechorionate them, followed by fixation in PBS, 3.7% formaldehyde and n-Heptane, dehydration with Methanol, rehydration with PBT (PBS with 0.3% Tritone added) before antibody staining. Visualisation was with DAB.

This image is courtesy of Dr Torsten Bossing at the University of Plymouth.

IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (AB12454)
  • IHC - Wmt

Characteriser16673****

IHC - Wholemount - Anti-Engrailed and Invected antibody [4D9] (AB12454)

ab12454 used in immunohistochemistry at 1/200 on Drosophila embryos.

Briefly, embryos were bleached (5% Bleach) to dechorionate them, followed by fixation in PBS, 3.7% formaldehyde and n-Heptane, dehydration with Methanol, rehydration with PBT (PBS with 0.3% Tritone added) before antibody staining. Visualisation was with DAB.

This image is courtesy of Dr Torsten Bossing at the University of Plymouth.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4D9

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Drosophila melanogaster

Applications

IHC - Wmt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

Epitope has been localized to residues 38-58 of the homeodomain.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCWmt-species-checked": "notRecommended", "IHCWmt-species-dilution-info": "1/200", "IHCWmt-species-notes": "<p>Fix with 4% paraformaldehyde in PBS for 1 hour.</p>" }, "Chicken": { "IHCWmt-species-checked": "notRecommended", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Crayfish": { "IHCWmt-species-checked": "notRecommended", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Drosophila melanogaster": { "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/200", "IHCWmt-species-notes": "<p>Fix with 4% paraformaldehyde in PBS for 1 hour.</p>" }, "Xenopus laevis": { "IHCWmt-species-checked": "notRecommended", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Zebrafish": { "IHCWmt-species-checked": "notRecommended", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Engrailed and Invected proteins often studied using the alternate name Engrailing function as homeodomain transcription factors. They play key roles in regulating gene expression and pattern formation during embryonic development. The Engrailed proteins have a molecular mass around 37 kDa and are expressed in several tissues including the central nervous system of Drosophila melanogaster. These proteins share structural similarities and often operate cooperatively in their functions.
Biological function summary

These transcription factors govern the segmentation and patterning processes critical for proper development. Engrailed and Invected proteins form part of the Engrailed/Hedgehog signaling complex which is important for maintaining the anterior-posterior axis in developing embryos. Through their interactions with other transcription factors and signaling molecules they ensure proper spatial and temporal expression of target genes important for tissue differentiation.

Pathways

A significant role for Engrailed and Invected proteins lies in the Hedgehog and Wingless signaling pathways. Engrailed assists in activating the Hedgehog pathway affecting cell fate decisions and patterning. Additionally Engrailed proteins interact with the Wingless signaling pathway where coordination with proteins like Cubitus interruptus leads to precise genetic regulation vital for maintaining developmental boundaries.

Abnormal expression or mutations in these proteins link to various developmental disorders and diseases. Misregulation of Engrailed and Invected can contribute to neoplastic conditions and disorders of the central nervous system. For instance links with proteins such as Patched and Smoothened in the Hedgehog pathway reveal their involvement in certain basal cell carcinomas when pathways fail to regulate cell growth properly.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Engrailed (en) and invected (inv) are functionally redundant transcription factors in neuronal precursor cell NB5-3 specification. Inv is unable to substitute for en in other regulatory processes such as maintaining gsb expression in the neuroectoderm after stage 10 of embryogenesis. Maintenance of gsb expression in row 5 of the neuroectoderm involves an as yet unidentified short range signaling molecule.
See full target information inv

Additional targets

en
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 37: PubMed29941661

2018

Engrailed homeoprotein blocks degeneration in adult dopaminergic neurons through LINE-1 repression.

Applications

Unspecified application

Species

Unspecified reactive species

François-Xavier Blaudin de Thé,Hocine Rekaik,Eugenie Peze-Heidsieck,Olivia Massiani-Beaudoin,Rajiv L Joshi,Julia Fuchs,Alain Prochiantz

Development genes and evolution 217:353-62 PubMed17394016

2007

Engrailed in cephalopods: a key gene related to the emergence of morphological novelties.

Applications

Unspecified application

Species

Unspecified reactive species

S Baratte,A Andouche,L Bonnaud

Developmental biology 258:44-56 PubMed12781681

2003

Evolution of identified arthropod neurons: the serotonergic system in relation to engrailed-expressing cells in the embryonic ventral nerve cord of the american lobster homarus americanus milne edwards, 1873 (malacostraca, pleocyemata, homarida).

Applications

Unspecified application

Species

Unspecified reactive species

Steffen Harzsch

The Journal of biological chemistry 277:46280-8 PubMed12215432

2002

Identification of a Drosophila gene encoding xylosylprotein beta4-galactosyltransferase that is essential for the synthesis of glycosaminoglycans and for morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yoko Nakamura,Nicola Haines,Jihua Chen,Tetsuya Okajima,Keiko Furukawa,Takeshi Urano,Pamela Stanley,Kenneth D Irvine,Koichi Furukawa

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 49:1177-82 PubMed11511686

2001

Simultaneous detection of RNA and protein by in situ hybridization and immunological staining.

Applications

Unspecified application

Species

Unspecified reactive species

H Nagaso,T Murata,N Day,K K Yokoyama

Proceedings of the National Academy of Sciences of the United States of America 97:10844-9 PubMed10995461

2000

Function of bicoid and hunchback homologs in the basal cyclorrhaphan fly Megaselia (Phoridae).

Applications

Unspecified application

Species

Unspecified reactive species

M Stauber,H Taubert,U Schmidt-Ott

Nature cell biology 1:144-51 PubMed10559900

1999

A new Drosophila APC homologue associated with adhesive zones of epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

X Yu,L Waltzer,M Bienz

Development (Cambridge, England) 126:2921-34 PubMed10357936

1999

Axial mesendoderm refines rostrocaudal pattern in the chick nervous system.

Applications

Unspecified application

Species

Unspecified reactive species

A M Rowan,C D Stern,K G Storey

Development (Cambridge, England) 126:577-86 PubMed9876186

1999

Wingless signaling in the Drosophila embryo: zygotic requirements and the role of the frizzled genes.

Applications

Unspecified application

Species

Unspecified reactive species

H A Müller,R Samanta,E Wieschaus

The Journal of cell biology 140:183-95 PubMed9425166

1998

Signaling and adhesion activities of mammalian beta-catenin and plakoglobin in Drosophila.

Applications

Unspecified application

Species

Unspecified reactive species

P White,H Aberle,J P Vincent
View all publications
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