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AB77273

Anti-Enkephalin/ENK antibody

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(4 Publications)

Goat Polyclonal Enkephalin antibody. Suitable for WB, IHC-FoFr and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Proenkephalin-A, PENK

1 Images
Western blot - Anti-Enkephalin/ENK antibody (AB77273)
  • WB

Unknown

Western blot - Anti-Enkephalin/ENK antibody (AB77273)

All lanes:

Western blot - Anti-Enkephalin/ENK antibody (ab77273) at 0.3 µg/mL

All lanes:

Human Adrenal Gland lysate in RIPA buffer at 35 µg

Predicted band size: 31 kDa

Observed band size: 37 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-FoFr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "predicted", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Enkephalin also known as ENK is a pentapeptide involved in regulating pain and emotion. It exists in two main forms: Leu-enkephalin and Met-enkephalin with molecular masses of 555.62 g/mol and 573.66 g/mol respectively. ENK peptides are primarily expressed in the central nervous system particularly in regions such as the thalamus hypothalamus and basal ganglia. They bind to opioid receptors especially the delta and mu receptors to modulate neurotransmission.
Biological function summary

Enkephalins play an important role in modulating pain perception and stress response. They do not operate alone but rather as components of a dynamic system involving other endogenous opioids and neuromodulators. Enkephalins participate in analgesic effects by binding to opioid receptors inhibiting neurotransmitter release and reducing neuronal excitability. Their interaction with other neurotransmitters including serotonin and norepinephrine suggests a broader role in emotional regulation and mood balance.

Pathways

Enkephalins engage in the opioid signaling pathway which influences pain and emotional states. This pathway interacts closely with the dopamine pathway where enkephalins modulate dopamine neurotransmission in areas related to reward and motivation. Other proteins such as the opioid receptors and prodynorphin play essential roles alongside enkephalins in these pathways contributing to complex interactions affecting mood and behavior.

Enkephalins relate to addiction and chronic pain conditions. They influence the rewarding effects of addictive substances interacting with dopamine receptor systems that can lead to dependency. Furthermore their involvement in pain pathways makes them relevant to the understanding and treatment of chronic pain disorders. Proteins like dynorphin another opioid peptide also link to these conditions often interacting with enkephalins to modulate pain and emotional response.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Met-enkephalin. Neuropeptide that competes with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.. Leu-enkephalin. Neuropeptide that competes with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.. Met-enkephalin-Arg-Phe. Met-enkephalin-Arg-Phe neuropeptide acts as a strong ligand of Mu-type opioid receptor OPRM1. Met-enkephalin-Arg-Phe-binding to OPRM1 in the nucleus accumbens of the brain increases activation of OPRM1, leading to long-term synaptic depression of glutamate release.. PENK(114-133). Increases glutamate release in the striatum and decreases GABA concentration in the striatum.. PENK(237-258). Increases glutamate release in the striatum.
See full target information PENK
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular neuroscience : MN 74:98 PubMed39414746

2024

Effects of Electroacupuncture at Varied Frequencies on Analgesia and Mechanisms in Sciatic Nerve Cuffing-Induced Neuropathic Pain Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kexin Fang,Wen Cheng,Bin Yu

Cancer management and research 11:6681-6690 PubMed31410059

2019

High expression of proenkephalin is associated with favorable outcomes in patients with gastrointestinal stromal tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Defeng Tang,Tianlong Lin,Yangyang Wang,Hui Cao

Molecular psychiatry 25:977-992 PubMed31142818

2019

Hippocampal µ-opioid receptors on GABAergic neurons mediate stress-induced impairment of memory retrieval.

Applications

Unspecified application

Species

Unspecified reactive species

Mei-Mei Shi,Ka-Min Fan,Yan-Ning Qiao,Jin-Hui Xu,Li-Juan Qiu,Xiao Li,Ying Liu,Zhao-Qiang Qian,Chun-Ling Wei,Jing Han,Juan Fan,Ying-Fang Tian,Wei Ren,Zhi-Qiang Liu

eNeuro 3: PubMed27517088

2016

Tolerance to Ethanol or Nicotine Results in Increased Ethanol Self-Administration and Long-Term Depression in the Dorsolateral Striatum.

Applications

Unspecified application

Species

Unspecified reactive species

Chandrika Abburi,Shannon L Wolfman,Ryan A E Metz,Rinya Kamber,Daniel S McGehee,John McDaid
View all publications
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