Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (AB155102)

Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labelling ENO1 + ENO2 + ENO3 with unpurified ab155102 at a dilution of 1/100.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (AB155102)

Intracellular Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling ENO1 with purified ab155102 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (AB155102)

Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling ENO1 + ENO2 + ENO3 with purified ab155102 at 1/60. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1 : primary antibody (1/60) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).

Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).

• IP

Lab

Immunoprecipitation - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (AB155102)

ab155102 (purified) at 1/20 immunoprecipitating ENO1 in HeLa whole cell lysate.

Lane 1 (input) : HeLa whole cell lysate (10μg)

Lane 2 (+) : ab155102 + HeLa whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab155102 in HeLa whole cell lysate.

For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (ab155102)

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

• WB

Unknown

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (AB155102)

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (ab155102) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 47 kDa

false

• WB

Lab

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (AB155102)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (ab155102) at 1/5000 dilution

Lane 1:

MCF-7 whole cell lysate at 10 µg

Lane 2:

Jurkat whole cell lysate at 10 µg

Lane 3:

HeLa whole cell lysate at 10 µg

Lane 4:

A431 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

• WB

Lab

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (AB155102)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (ab155102) at 1/5000 dilution

Lane 1:

C2C12 whole cell lysate at 10 µg

Lane 2:

NIH/3T3 whole cell lysate at 10 µg

Lane 3:

Mouse heart tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

• WB

Lab

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (AB155102)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (ab155102) at 1/5000 dilution

All lanes:

Rat brain tissue lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/1000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

• WB

Lab

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (AB155102)

Mouse anti-6x HisTag was used as a loading control.

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (ab155102) at 1/5000 dilution

Lane 1:

Human ENO3 protein (His Tag) at 0.2 µg

Lane 2:

Human ENO2 Protein (His Tag) at 0.2 µg

Lane 3:

Human ENO1 Protein (His Tag) at 0.2 µg

Predicted band size: 47 kDa

false