Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] - BSA and Azide free (AB206120)

Clone EPR10863(B) (ab206120) has been successfully conjugated by Abcam. This image was generated using Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (Alexa Fluor® 647). Please refer to ab205872 for protocol details.

ab205872 staining ENO1 + ENO2 + ENO3 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205872 at a 1/250 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] - BSA and Azide free (AB206120)

Clone EPR10863(B) (ab206120) has been successfully conjugated by Abcam. This image was generated using Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (Alexa Fluor® 488). Please refer to ab205871 for protocol details.

ab205871 staining ENO1 + ENO2 + ENO3 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205871 at a 1/250 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] - BSA and Azide free (AB206120)

Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labelling ENO1 + ENO2 + ENO3 with unpurified ab155102 at a dilution of 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155102).

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] - BSA and Azide free (AB206120)

Intracellular Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling ENO1 with purified ab155102 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155102).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] - BSA and Azide free (AB206120)

Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling ENO1 + ENO2 + ENO3 with purified ab155102 at 1/60. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1 : primary antibody (1/60) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).

Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155102).

• IP

Lab

Immunoprecipitation - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] - BSA and Azide free (AB206120)

ab155102 (purified) at 1/20 immunoprecipitating ENO1 in HeLa whole cell lysate.

Lane 1 (input) : HeLa whole cell lysate (10µg)

Lane 2 (+) : ab155102 + HeLa whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab155102 in HeLa whole cell lysate.

For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155102).

All lanes:

Immunoprecipitation - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (<a href='/en-us/products/primary-antibodies/eno1-eno2-eno3-antibody-epr10863b-ab155102'>ab155102</a>)

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

• WB

Lab

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] - BSA and Azide free (AB206120)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155102).

Mouse anti-6x HisTag was used as a loading control.

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (<a href='/en-us/products/primary-antibodies/eno1-eno2-eno3-antibody-epr10863b-ab155102'>ab155102</a>) at 1/5000 dilution

Lane 1:

Human ENO3 protein (His Tag) at 0.2 µg

Lane 2:

Human ENO2 Protein (His Tag) at 0.2 µg

Lane 3:

Human ENO1 Protein (His Tag) at 0.2 µg

Predicted band size: 47 kDa

false